KEGG   ENZYME: 1.6.1.4
Entry
EC 1.6.1.4                  Enzyme                                 
Name
NAD(P)+ transhydrogenase (ferredoxin);
NADH-dependent reduced ferredoxin:NADP+ oxidoreductase;
Nfn;
nfnAB (gene names)
Class
Oxidoreductases;
Acting on NADH or NADPH;
With NAD+ or NADP+ as acceptor
Sysname
NADH:NADP+, ferredoxin oxidoreductase
Reaction(IUBMB)
NADH + H+ + 2 NADP+ + 2 reduced ferredoxin [iron-sulfur] cluster = NAD+ + 2 NADPH + 2 oxidized ferredoxin [iron-sulfur] cluster
Substrate
NADH [CPD:C00004];
H+ [CPD:C00080];
NADP+ [CPD:C00006];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138]
Product
NAD+ [CPD:C00003];
NADPH [CPD:C00005];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139]
Comment
The iron-sulfur flavoprotein complex, originally isolated from the bacterium Clostridium kluyveri, couples the exergonic reduction of NADP+ with reduced ferredoxin and the endergonic reduction of NADP+ with NADH.
History
EC 1.6.1.4 created 2015
Reference
1  [PMID:20675474]
  Authors
Wang S, Huang H, Moll J, Thauer RK
  Title
NADP+ reduction with reduced ferredoxin and NADP+ reduction with NADH are coupled via an electron-bifurcating enzyme complex in Clostridium kluyveri.
  Journal
J Bacteriol 192:5115-23 (2010)
DOI:10.1128/JB.00612-10
Reference
2  [PMID:26139605]
  Authors
Demmer JK, Huang H, Wang S, Demmer U, Thauer RK, Ermler U
  Title
Insights into Flavin-based Electron Bifurcation via the NADH-dependent Reduced Ferredoxin:NADP Oxidoreductase Structure.
  Journal
J Biol Chem 290:21985-95 (2015)
DOI:10.1074/jbc.M115.656520
Reference
3  [PMID:28394885]
  Authors
Lubner CE, Jennings DP, Mulder DW, Schut GJ, Zadvornyy OA, Hoben JP, Tokmina-Lukaszewska M, Berry L, Nguyen DM, Lipscomb GL, Bothner B, Jones AK, Miller AF, King PW, Adams MWW, Peters JW
  Title
Mechanistic insights into energy conservation by flavin-based electron bifurcation.
  Journal
Nat Chem Biol 13:655-659 (2017)
DOI:10.1038/nchembio.2348
Other DBs
ExplorEnz - The Enzyme Database: 1.6.1.4
IUBMB Enzyme Nomenclature: 1.6.1.4
ExPASy - ENZYME nomenclature database: 1.6.1.4
BRENDA, the Enzyme Database: 1.6.1.4

  All links  
Protein sequence (4)   
   PDBSTR (4)   
DNA sequence (2)   
   GenBank (1)   
   EMBL (1)   
3D Structure (2)   
   PDB (2)   
All databases (8)   

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