KEGG ENZYME: 1.7.1.16

ENZYME: 1.7.1.16

Entry

EC 1.7.1.16                 Enzyme

Name

nitrobenzene nitroreductase;
cnbA (gene name)

Class

Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With NAD+ or NADP+ as acceptor

Sysname

N-phenylhydroxylamine:NADP+ oxidoreductase

Reaction(IUBMB)

N-phenylhydroxylamine + 2 NADP+ + H2O = nitrobenzene + 2 NADPH + 2 H+ (overall reaction) [RN:R11744];
(1a) N-phenylhydroxylamine + NADP+ = nitrosobenzene + NADPH + H+ [RN:R05400];
(1b) nitrosobenzene + NADP+ + H2O = nitrobenzene + NADPH + H+ [RN:R05401]

Reaction(KEGG)

R05400 R05401 R11744

Substrate

N-phenylhydroxylamine [CPD:C02720];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
nitrosobenzene [CPD:C06876]

Product

nitrobenzene [CPD:C06813];
NADPH [CPD:C00005];
H+ [CPD:C00080];
nitrosobenzene [CPD:C06876]

Comment

Contains FMN. The enzyme, characterized from Pseudomonas species, catalyses two successive reductions of nitrobenzene, via a nitrosobenzene intermediate. It is also active on 1-chloro-4-nitrobenzene.

History

EC 1.7.1.16 created 2017

Pathway

ec00627

Aminobenzoate degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K15057

nitrobenzene nitroreductase

Genes

RPJ:	N234_22330

REH:	H16_B1416(h16_B1416)

REU:	Reut_C5984

COX:	E0W60_03595

CCAM:

M5D45_20080

CUK:	KB879_34770

VAP:	Vapar_5417

VPD:	VAPA_2c08880

ECAA:

J3R84_25755

RHR:	CKA34_21630

RGR:	FZ934_23955

» show all

Reference

1 [PMID:7601851]

Authors

Somerville CC, Nishino SF, Spain JC

Title

Purification and characterization of nitrobenzene nitroreductase from Pseudomonas pseudoalcaligenes JS45.

Journal

J Bacteriol 177:3837-42 (1995)
DOI:10.1128/JB.177.13.3837-3842.1995

Reference

2 [PMID:16517619]

Authors

Wu JF, Jiang CY, Wang BJ, Ma YF, Liu ZP, Liu SJ

Title

Novel partial reductive pathway for 4-chloronitrobenzene and nitrobenzene degradation in Comamonas sp. strain CNB-1.

Journal

Appl Environ Microbiol 72:1759-65 (2006)
DOI:10.1128/AEM.72.3.1759-1765.2006

Other DBs

ExplorEnz - The Enzyme Database:

1.7.1.16

IUBMB Enzyme Nomenclature:

1.7.1.16

ExPASy - ENZYME nomenclature database:

1.7.1.16

BRENDA, the Enzyme Database:

1.7.1.16

DBGET integrated database retrieval system