Entry
Name
cysteine-type anaerobic sulfatase-maturating enzyme;
anSME;
Cys-type anaerobic sulfatase-maturating enzyme;
anaerobic sulfatase maturase
Class
Oxidoreductases;
Acting on a sulfur group of donors;
With other, known, physiological acceptors
BRITE hierarchy
Sysname
[sulfatase]-L-cysteine:S-adenosyl-L-methionine oxidoreductase (3-oxo-L-alanine-forming)
Reaction(IUBMB)
S-adenosyl-L-methionine + a [sulfatase]-L-cysteine + H2O = a [sulfatase]-3-oxo-L-alanine + 5'-deoxyadenosine + L-methionine + hydrogen sulfide
Substrate
S-adenosyl-L-methionine [CPD:
C00019 ];
[sulfatase]-L-cysteine [CPD:
C21740 ];
H2O [CPD:
C00001 ]
Product
[sulfatase]-3-oxo-L-alanine [CPD:
C21741 ];
5'-deoxyadenosine [CPD:
C05198 ];
L-methionine [CPD:
C00073 ];
hydrogen sulfide [CPD:
C00283 ]
Comment
A radical S-adenosylmethionine (AdoMet) enzyme that contains three [4Fe-4S] clusters. The enzyme, found in some bacteria, activates a type I sulfatase enzyme (EC
3.1.6.1 ) by converting a conserved L-cysteine residue in the active site to a unique 3-oxo-L-alanine residue that is essential for the sulfatase activity. Some enzymes can also act on L-serine, see EC
1.1.98.7 , serine-type anaerobic sulfatase-maturating enzyme and EC
1.8.3.7 , formylglycine-generating enzyme.
History
EC 1.8.98.7 created 2020
Reference
Authors
Berteau O, Guillot A, Benjdia A, Rabot S
Title
A new type of bacterial sulfatase reveals a novel maturation pathway in prokaryotes.
Journal
Sequence
Reference
Authors
Benjdia A, Subramanian S, Leprince J, Vaudry H, Johnson MK, Berteau O
Title
Anaerobic sulfatase-maturating enzymes, first dual substrate radical S-adenosylmethionine enzymes.
Journal
Sequence
Reference
Authors
Benjdia A, Leprince J, Sandstrom C, Vaudry H, Berteau O
Title
Mechanistic investigations of anaerobic sulfatase-maturating enzyme: direct Cbeta H-atom abstraction catalyzed by a radical AdoMet enzyme.
Journal
Reference
Authors
Benjdia A, Subramanian S, Leprince J, Vaudry H, Johnson MK, Berteau O
Title
Anaerobic sulfatase-maturating enzyme--a mechanistic link with glycyl radical-activating enzymes?
Journal
Reference
Authors
Grove TL, Ahlum JH, Qin RM, Lanz ND, Radle MI, Krebs C, Booker SJ
Title
Further characterization of Cys-type and Ser-type anaerobic sulfatase maturating enzymes suggests a commonality in the mechanism of catalysis.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.8.98.7
ExPASy - ENZYME nomenclature database: 1.8.98.7