KEGG ENZYME: 2.1.1.102

ENZYME: 2.1.1.102

Entry

EC 2.1.1.102                Enzyme

Name

demethylmacrocin O-methyltransferase;
demethylmacrocin methyltransferase;
DMOMT

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:demethylmacrocin 2'''-O-methyltransferase

Reaction(IUBMB)

S-adenosyl-L-methionine + demethylmacrocin = S-adenosyl-L-homocysteine + macrocin [RN:R02859]

Reaction(KEGG)

R02859

Substrate

S-adenosyl-L-methionine [CPD:C00019];
demethylmacrocin [CPD:C02400]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
macrocin [CPD:C00744]

Comment

Requires Mg2+. The enzyme, isolated from the bacterium Streptomyces fradiae, is involved in the biosynthesis of the macrolide antibiotic tylosin. The 2-hydroxy group of a 6-deoxy-D-allose moiety in demethylmacrocin acts as the methyl acceptor. Also acts on demethyllactenocin, giving lactenocin. Not identical with EC 2.1.1.101 macrocin O-methyltransferase.

History

EC 2.1.1.102 created 1992

Pathway

ec00522

Biosynthesis of 12-, 14- and 16-membered macrolides

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K15995

demethylmacrocin O-methyltransferase

Reference

1 [PMID:3170602]

Authors

Kreuzman AJ, Turner JR, Yeh WK

Title

Two distinctive O-methyltransferases catalyzing penultimate and terminal reactions of macrolide antibiotic (tylosin) biosynthesis. Substrate specificity, enzyme inhibition, and kinetic mechanism.

Journal

J Biol Chem 263:15626-33 (1988)

Sequence

[ag:AAD12164]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.102

IUBMB Enzyme Nomenclature:

2.1.1.102

ExPASy - ENZYME nomenclature database:

2.1.1.102

BRENDA, the Enzyme Database:

2.1.1.102

CAS:	120313-64-6

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