KEGG ENZYME: 2.1.1.209

ENZYME: 2.1.1.209

Entry

EC 2.1.1.209                Enzyme

Name

23S rRNA (guanine2535-N1)-methyltransferase;
AviRa

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:23S rRNA (guanine2535-N1)-methyltransferase

Reaction(IUBMB)

S-adenosyl-L-methionine + guanine2535 in 23S rRNA = S-adenosyl-L-homocysteine + N1-methylguanine2535 in 23S rRNA

Substrate

S-adenosyl-L-methionine [CPD:C00019];
guanine2535 in 23S rRNA

Product

S-adenosyl-L-homocysteine [CPD:C00021];
N1-methylguanine2535 in 23S rRNA

Comment

Streptomyces viridochromogenes produces the antibiotic avilamycin A which binds to the 50S ribosomal subunit to inhibit protein synthesis. To protect itself from the antibiotic, Streptomyces viridochromogenes utilizes two methyltransferases, 23S rRNA (guanine2535-N1)-methyltransferase and EC 2.1.1.208 [23S rRNA (uridine2479-2-O)-methyltransferase], whose actions confer avilamycin resistance to the RNA.

History

EC 2.1.1.209 created 2011

Orthology

K21515

23S rRNA (guanine2535-N1)-methyltransferase

Genes

ROY:	G3A56_02055

BLE:	BleG1_3359

SHUA:

PQ477_04435

GYM:	GYMC10_5407

PIB:	BBD41_19095

PLW:	D5F53_03085

PALB:

EJC50_10290

PRZ:	GZH47_00155

PLYC:

GXP70_06190

PCEL:

HUB94_03425

PSPN:

L1F29_32555

TCO:	Theco_1547

TVU:	AB849_009220

THL:

TEH_05890

TEY:	GLW17_09200

TOO:	C7K38_06580

CIA:	BEN51_12445

CINT:

HZF06_08255

CTHD:

CDO33_15870

CPY:

Cphy_2160

ANR:	Ana3638_22340

ACHT:

bsdcttw_31380

SMOB:

J7W19_17790

SKE:	Sked_37520

NDA:

Ndas_4431

NAL:	B005_2412(aviRa)

NGV:	CDO52_26725

NEX:	NE857_03610

NEC:	KGD82_25360

NAKE:

KGD83_02860

NCG:	KGD84_29345

STRR:

EKD16_23275(aviRa)

SNAH:

OUQ99_29335

AMAZ:

LUW76_02335

SRO:

Sros_4106

NCX:	Nocox_42140(aviRa)

NFS:	OIE67_00865

MHAI:

OHB01_15605

PSEA:

WY02_14740

PSEE:

FRP1_27745

PSEH:

XF36_26020

PSEQ:

AD006_06925

PECQ:

AD017_14740

PHH:	AFB00_12795

PAUT:

Pdca_64250

KBU:	Q4V64_26070

AHM:	TL08_09860

ACTI:

UA75_10110

ACAD:

UA74_10135

AHG:	AHOG_09540(aviRa)

ACTA:

C1701_04110

MCAB:

HXZ27_04110(evdX3)

DROS:

Drose_26660

KBS:	EPA93_05725

PMET:

G4Y79_03735

» show all

Reference

1 [PMID:12828631]

Authors

Treede I, Jakobsen L, Kirpekar F, Vester B, Weitnauer G, Bechthold A, Douthwaite S

Title

The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the guanosine 2535 base and the uridine 2479 ribose.

Journal

Mol Microbiol 49:309-18 (2003)
DOI:10.1046/j.1365-2958.2003.03558.x

Sequence

[ag:AAG32067]

Reference

2 [PMID:11181344]

Authors

Weitnauer G, Gaisser S, Trefzer A, Stockert S, Westrich L, Quiros LM, Mendez C, Salas JA, Bechthold A

Title

An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tu57.

Journal

Antimicrob Agents Chemother 45:690-5 (2001)
DOI:10.1128/AAC.45.3.690-695.2001

Sequence

[ag:AAG32067]

Reference

3 [PMID:12742024]

Authors

Mosbacher TG, Bechthold A, Schulz GE

Title

Crystal structure of the avilamycin resistance-conferring methyltransferase AviRa from Streptomyces viridochromogenes.

Journal

J Mol Biol 329:147-57 (2003)
DOI:10.1016/S0022-2836(03)00407-8

Sequence

[ag:AAG32067]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.209

IUBMB Enzyme Nomenclature:

2.1.1.209

ExPASy - ENZYME nomenclature database:

2.1.1.209

BRENDA, the Enzyme Database:

2.1.1.209

All links

Gene (71)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (56)   
   KEGG MGENES (8)   
   RefGene (6)   
Protein sequence (10)   
   UniProt (9)   
   SWISS-PROT (1)   
DNA sequence (8)   
   GenBank (4)   
   EMBL (4)   
Protein domain (2)   
   InterPro (2)   
All databases (91)   

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