KEGG   ENZYME: 2.1.1.267
Entry
EC 2.1.1.267                Enzyme                                 
Name
flavonoid 3',5'-methyltransferase;
AOMT;
CrOMT2
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:flavonoid 3'-O-methyltransferase
Reaction(IUBMB)
(1) S-adenosyl-L-methionine + a 3'-hydroxyflavonoid = S-adenosyl-L-homocysteine + a 3'-methoxyflavonoid;
(2) S-adenosyl-L-methionine + a 5'-hydroxy-3'-methoxyflavonoid = S-adenosyl-L-homocysteine + a 3',5'-dimethoxyflavonoid
Reaction(KEGG)
(other) R06815 R06816
Substrate
S-adenosyl-L-methionine [CPD:C00019];
3'-hydroxyflavonoid [CPD:C02790];
5'-hydroxy-3'-methoxyflavonoid
Product
S-adenosyl-L-homocysteine [CPD:C00021];
3'-methoxyflavonoid;
3',5'-dimethoxyflavonoid
Comment
Isolated from Vitis vinifera (grape) [2]. Most active with delphinidin 3-glucoside but also acts on cyanidin 3-glucoside, cyanidin, myricetin, quercetin and quercetin 3-glucoside. The enzyme from Catharanthus roseus was most active with myricetin [1].
History
EC 2.1.1.267 created 2013, modified 2014
Pathway
ec00944  Flavone and flavonol biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13272  flavonoid O-methyltransferase
Genes
HSYR120138721 120197908
PDUL117616544 117619928
MSYL126595165 126611729
CSAV115712603 115712971
CILL122309323
CAVE132192083
QLO115982101 115982433
VVI100233134(FAOMT) 100245452 100250567 100250579
EGT105958570 105958584
SSPL121793763 121798458
APAN127244939
CSIN114275659 114293531 114305583 114312686 114317032
RVL131311302 131311303 131311304
AEW130771302 130771738
ATRI130809233 130809234
MING122079574
TSS122647755 122652785 122652787 122663698
 » show all
Reference
1  [PMID:12482447]
  Authors
Cacace S, Schroder G, Wehinger E, Strack D, Schmidt J, Schroder J
  Title
A flavonol O-methyltransferase from Catharanthus roseus performing two sequential methylations.
  Journal
Phytochemistry 62:127-37 (2003)
DOI:10.1016/S0031-9422(02)00483-1
  Sequence
Reference
2  [PMID:19525322]
  Authors
Hugueney P, Provenzano S, Verries C, Ferrandino A, Meudec E, Batelli G, Merdinoglu D, Cheynier V, Schubert A, Ageorges A
  Title
A novel cation-dependent O-methyltransferase involved in anthocyanin methylation in grapevine.
  Journal
Plant Physiol 150:2057-70 (2009)
DOI:10.1104/pp.109.140376
  Sequence
[vvi:100233134]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.267
IUBMB Enzyme Nomenclature: 2.1.1.267
ExPASy - ENZYME nomenclature database: 2.1.1.267
BRENDA, the Enzyme Database: 2.1.1.267

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