KEGG ENZYME: 2.1.1.296

ENZYME: 2.1.1.296

Entry

EC 2.1.1.296                Enzyme

Name

methyltransferase cap2;
CMTR2 (gene name);
MTR2;
cap2-MTase;
mRNA (nucleoside-2'-O)-methyltransferase (ambiguous)

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-ribonucleotide-[mRNA] 2'-O-methyltransferase

Reaction(IUBMB)

S-adenosyl-L-methionine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-(2'-O-methyl-ribonucleotide)-[mRNA] [RN:R10812]

Reaction(KEGG)

R10812

Substrate

S-adenosyl-L-methionine [CPD:C00019];
5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-(ribonucleotide)-[mRNA]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-(2'-O-methyl-ribonucleotide)-[mRNA]

Comment

The enzyme, found in higher eukaryotes including insects and vertebrates, and their viruses, methylates the ribose of the ribonucleotide at the second transcribed position of mRNAs and snRNAs. This methylation event is known as cap2. The human enzyme can also methylate mRNA molecules where the upstream ribonucleotide is not methylated (see EC 2.1.1.57, methyltransferase cap1), but with lower efficiency [2].

History

EC 2.1.1.296 created 2014, modified 2021

Orthology

K14590

cap2 methyltransferase

K18776

cap2 methyltransferase

Genes

HSA:	55783(CMTR2)

PTR:	745202(CMTR2)

PPS:	100985401(CMTR2)

GGO:	101137210(CMTR2)

PON:	100172540(CMTR2)

NLE:	100579817(CMTR2)

HMH:	116471477(CMTR2)

MCC:	711529(CMTR2)

MCF:	102120594(CMTR2)

MTHB:

126943718

MNI:	105491297(CMTR2)

CSAB:

103233165(CMTR2)

CATY:

105576570(CMTR2)

PANU:

101000385(CMTR2)

TGE:	112614594(CMTR2)

MLEU:

105537212(CMTR2)

RRO:	104677811(CMTR2)

RBB:	108527451(CMTR2)

TFN:	117088023(CMTR2)

PTEH:

111541436(CMTR2)

CANG:

105526139(CMTR2)

CJC:	100412690(CMTR2)

SBQ:	101045320(CMTR2)

CIMI:

108311671(CMTR2)

CSYR:

103274659(CMTR2)

MMUR:

105875419(CMTR2)

LCAT:

123625459(CMTR2)

PCOQ:

105807986(CMTR2)

OGA:	100946517(CMTR2)

MMU:	234728(Cmtr2)

MCAL:

110300394(Cmtr2)

MPAH:

110337588(Cmtr2)

RNO:	292016(Cmtr2)

MCOC:

116070169(Cmtr2)

ANU:	117723532(Cmtr2)

MUN:	110542580 110546039(Cmtr2)

CGE:	100754099(Cmtr2)

MAUA:

101844558(Cmtr2)

PROB:

127234446(Cmtr2)

PLEU:

114708075(Cmtr2)

MORG:

121447103(Cmtr2)

MFOT:

126502986

AAMP:

119801949(Cmtr2)

NGI:	103735630(Cmtr2)

HGL:	101696697(Cmtr2)

CPOC:

100718778(Cmtr2)

CCAN:

109678265 109703294

DORD:

105983171(Cmtr2)

DSP:	122126207(Cmtr2)

PLOP:

125358208(Cmtr2)

NCAR:

124966848

MMMA:

107150923(Cmtr2)

OCU:

100346528

OPI:	101519355(CMTR2)

TUP:	102482679(CMTR2)

GVR:	103592477(CMTR2)

CFA:	489718(CMTR2)

CLUD:

112646611(CMTR2)

VVP:	112929495(CMTR2)

VLG:	121500707(CMTR2)

NPO:	129497846(CMTR2)

AML:	100482851(CMTR2)

UMR:	103673582(CMTR2)

UAH:	113252464(CMTR2)

UAR:	123800537(CMTR2)

ELK:

111153016

LLV:

125089289

MPUF:

101671552(CMTR2)

MNP:	132005245(CMTR2)

MLK:	131817518(CMTR2)

NVS:	122911264(CMTR2)

ORO:	101385736(CMTR2)

EJU:	114202848(CMTR2)

ZCA:	113936499(CMTR2)

MLX:	118023594(CMTR2)

NSU:	110592151(CMTR2)

LWW:	102739153(CMTR2)

FCA:	101081592(CMTR2)

PYU:	121009766(CMTR2)

PCOO:

112857494(CMTR2)

PBG:	122494436(CMTR2)

PVIV:

125153193(CMTR2)

LRUF:

124511467

PTG:	102969106(CMTR2)

PPAD:

109270657(CMTR2)

PUC:

125916410

AJU:

106986674

HHV:	120225421(CMTR2)

BTA:	781914(CMTR2)

BOM:	102269376(CMTR2)

BIU:	109572848(CMTR2)

BBUB:

102413700(CMTR2)

BBIS:

104996618(CMTR2)

CHX:	102182998(CMTR2)

OAS:	101106100(CMTR2)

BTAX:

128063869(CMTR2)

ODA:	120873254(CMTR2)

CCAD:

122421188(CMTR2)

MBEZ:

129546925(CMTR2)

SSC:	100625187(CMTR2)

CFR:	102518757(CMTR2)

CBAI:

105073551(CMTR2)

CDK:	105104273(CMTR2)

VPC:	102540362(CMTR2)

BACU:

103017175(CMTR2)

BMUS:

118885523(CMTR2)

LVE:	103086480(CMTR2)

OOR:	101277993(CMTR2)

DLE:	111179544(CMTR2)

PCAD:

102992875(CMTR2)

PSIU:

116743995(CMTR2)

NASI:

112393371(CMTR2)

ECB:	100068485(CMTR2)

EPZ:	103551750(CMTR2)

EAI:	106830912(CMTR2)

MYB:	102252269(CMTR2)

MYD:	102758049(CMTR2)

MMYO:

118674099(CMTR2)

MLF:	102420549(CMTR2)

PKL:	118720767(CMTR2)

EFUS:

103284647(CMTR2)

MNA:	107524398(CMTR2)

DRO:	112300833(CMTR2)

SHON:

118999240(CMTR2)

AJM:	119050196 119050197

PDIC:

114488103(CMTR2)

PHAS:

123806060(CMTR2)

MMF:	118639556(CMTR2)

PPAM:

129075060(CMTR2)

HAI:	109388964(CMTR2)

RFQ:	117035468(CMTR2)

PALE:

102893817(CMTR2)

PGIG:

120593965(CMTR2)

PVP:	105305835(CMTR2)

RAY:	107520520(CMTR2)

MJV:	108389797(CMTR2)

TOD:	119249043(CMTR2)

SETR:

126027357(CMTR2)

LAV:	100671400(CMTR2)

TMU:

101340596

ETF:	101648565(CMTR2)

DNM:	101414121(CMTR2)

MDO:	100024780(CMTR2)

GAS:	123234563(CMTR2)

SHR:	100914522(CMTR2)

AFZ:

127547643

PCW:	110192470(CMTR2)

OAA:	100074514(CMTR2)

GGA:	427537(CMTR2)

PCOC:

116243878(CMTR2)

MGP:	100550810(CMTR2)

CJO:	107319188(CMTR2)

TPAI:

128081789(CMTR2)

LMUT:

125699200(CMTR2)

NMEL:

110404401(CMTR2)

APLA:

113844804(CMTR2)

ACYG:

106033270(CMTR2)

CATA:

118246581(CMTR2)

AFUL:

116494149(CMTR2)

TGU:	100230404(CMTR2)

LSR:	110476247(CMTR2)

SCAN:

103816673(CMTR2)

PMOA:

120508135(CMTR2)

OTC:	121333899(CMTR2)

PRUF:

121349014(CMTR2)

GFR:	102044295(CMTR2)

FAB:	101817347(CMTR2)

OMA:	130257626(CMTR2)

PHI:	102112750(CMTR2)

PMAJ:

107210044(CMTR2)

CCAE:

111934531(CMTR2)

CCW:	104687521(CMTR2)

CBRC:

103620280(CMTR2)

ETL:	114063438(CMTR2)

ZAB:	102070844(CMTR2)

ACHL:

103807775(CMTR2)

SVG:	106855794(CMTR2)

MMEA:

130579572(CMTR2)

HRT:	120758011(CMTR2)

FPG:	101913175(CMTR2)

FCH:	102045864(CMTR2)

CLV:	102092901(CMTR2)

EGZ:	104130241(CMTR2)

NNI:	104017447(CMTR2)

PCRI:

104027856(CMTR2)

PLET:

104617478(CMTR2)

EHS:	104502653(CMTR2)

PCAO:

104053665(CMTR2)

ACUN:

113484389(CMTR2)

TALA:

104367875(CMTR2)

PADL:

103922258(CMTR2)

AFOR:

103908409(CMTR2)

ACHC:

115346255(CMTR2)

HALD:

104313090(CMTR2)

HLE:	104841965(CMTR2)

AGEN:

126040313

GCL:

127021112

CCRI:

104159260(CMTR2)

CSTI:

104555531(CMTR2)

CMAC:

104477667(CMTR2)

MUI:	104533171(CMTR2)

BREG:

104637850(CMTR2)

FGA:	104069075(CMTR2)

GSTE:

104250226(CMTR2)

LDI:	104348619(CMTR2)

MNB:	103781296(CMTR2)

OHA:	104328588(CMTR2)

NNT:	104411372(CMTR2)

SHAB:

115619759(CMTR2)

DPUB:

104298772(CMTR2)

PGUU:

104469924(CMTR2)

ACAR:

104527375(CMTR2)

CPEA:

104389051(CMTR2)

AVIT:

104277465(CMTR2)

CVF:	104284886(CMTR2)

RTD:	128908582(CMTR2)

CUCA:

104056812(CMTR2)

TEO:	104373695(CMTR2)

BRHI:

104489931(CMTR2)

AAM:	106489389(CMTR2)

AROW:

112975169(CMTR2)

NPD:	112943265(CMTR2)

TGT:	104578228(CMTR2)

DNE:	112987455(CMTR2)

SCAM:

104147164(CMTR2)

ASN:	102379025(CMTR2)

AMJ:	102567886(CMTR2)

CPOO:

109310445(CMTR2)

GGN:	109289677(CMTR2)

PSS:	102462904(CMTR2)

CMY:	102941834(CMTR2)

CCAY:

125620744(CMTR2)

DCC:	119841117(CMTR2)

CPIC:

101933286(CMTR2)

TST:	117886600(CMTR2)

CABI:

116833710(CMTR2)

MRV:	120384572(CMTR2)

ACS:	100566866(cmtr2)

ASAO:

132772232(CMTR2)

PVT:	110091268(CMTR2)

SUND:

121926880(CMTR2)

PBI:	103049103(CMTR2)

PMUR:

107298304(CMTR2)

CTIG:

120305829(CMTR2)

TSR:	106541444(CMTR2)

PGUT:

117660483(CMTR2)

APRI:

131199754(CMTR2)

PTEX:

113440442(CMTR2)

NSS:	113420122(CMTR2)

VKO:	123029482(CMTR2)

PMUA:

114595624(CMTR2)

PRAF:

128412546(CMTR2)

ZVI:	118085705(CMTR2)

HCG:	128350285(CMTR2)

GJA:	107109618(CMTR2)

STOW:

125431447(CMTR2)

EMC:	129325411(CMTR2)

XLA:	108714154(cmtr2.L)

XTR:	100496665(cmtr2)

NPR:	108802960(CMTR2)

RTEM:

120917773(CMTR2)

BBUF:

120980744(CMTR2)

BGAR:

122920491(CMTR2)

MUO:	115456828(CMTR2)

GSH:	117348983(CMTR2)

DRE:	406742(cmtr2)

SRX:

107749817

SANH:

107691918

SGH:

107588175

CCAR:

109093042

CAUA:

113052104 113105817

CGIB:

127961143 128017115

PTET:

122349761(cmtr2)

LROH:

127168849(cmtr2)

OMC:	131545057(cmtr2)

PPRM:

120469948(cmtr2)

RKG:	130102594(cmtr2)

MAMB:

125264551(cmtr2)

CIDE:

127516092

MASI:

127414691 127448471

TROS:

130554438(cmtr2)

TDW:	130430189(cmtr2)

MANU:

129440589(cmtr2)

IPU:

108270675

IFU:	128614174(cmtr2)

PHYP:

113523947(cmtr2)

SMEO:

124391596(cmtr2)

TFD:	113646175(cmtr2)

TVC:	132850430(cmtr2)

AMEX:

103026585(cmtr2)

CMAO:

118818940(cmtr2)

EEE:	113586074(cmtr2)

CHAR:

105907229(cmtr2)

TRU:	101076967(cmtr2)

TFS:	130521622(cmtr2)

TNG:	GSTEN00017682G001

LCO:	104927813(cmtr2)

NCC:	104965821(cmtr2)

TBEN:

117471893(cmtr2)

CGOB:

115006392(cmtr2)

PGEO:

117461134(cmtr2)

GACU:

117533248(cmtr2)

EMAC:

134863843(cmtr2)

ELY:	117261638(cmtr2)

EFO:	125879378(cmtr2)

PLEP:

121956898(cmtr2)

SLUC:

116060679(cmtr2)

ECRA:

117946202(cmtr2)

ESP:	116690846(cmtr2)

PFLV:

114553970(cmtr2)

GAT:	120811999(cmtr2)

PPUG:

119197166(cmtr2)

AFB:	129104729(cmtr2)

CLUM:

117728658(cmtr2)

PSWI:

130192523(cmtr2)

MSAM:

119890295 119896808

SCHU:

122878005(cmtr2)

CUD:	121513282(cmtr2)

ALAT:

119018469(cmtr2)

MZE:	101481599(cmtr2)

ONL:	100706104(cmtr2)

OAU:	116334637(cmtr2)

OLA:	101158006(cmtr2)

OML:	112161237(cmtr2)

CSAI:

133462657(cmtr2)

XMA:	102226934(cmtr2)

XCO:	114143795(cmtr2)

XHE:	116718047(cmtr2)

PRET:

103462552(cmtr2)

PFOR:

103136592(cmtr2)

PLAI:

106948994(cmtr2)

PMEI:

106921979(cmtr2)

GAF:	122843304(cmtr2)

PPRL:

129364016(cmtr2)

CVG:	107103775(cmtr2)

CTUL:

119798987(cmtr2)

GMU:	124866440(cmtr2)

NFU:	107381339(cmtr2)

KMR:	108251077(cmtr2)

ALIM:

106532123(cmtr2)

NWH:	119428506(cmtr2)

AOCE:

111573313(cmtr2)

MCEP:

125004901(cmtr2)

CSEM:

103378880(cmtr2)

POV:	109636722(cmtr2)

SSEN:

122772837(cmtr2)

HHIP:

117759239(cmtr2)

HSP:	118112306(cmtr2)

SMAU:

118302541(cmtr2)

LCF:

108877776

SDU:	111219044(cmtr2)

SLAL:

111646243(cmtr2)

XGL:	120793185(cmtr2)

HCQ:	109526031(cmtr2)

SSCV:

125967341

SBIA:

133498107(cmtr2)

PEE:	133396090(slc38a8b)

PTAO:

133474423(cmtr2)

BPEC:

110175448(cmtr2)

MALB:

109966039(cmtr2)

BSPL:

114852508(cmtr2)

SJO:	128362389(cmtr2)

SASA:

106562241(cmtr2)

STRU:

115203133(cmtr2)

OTW:	112263738(cmtr2)

OMY:

110506576

OGO:	124035693(cmtr2)

ONE:

115112304

OKI:	109882454(cmtr2)

OKE:

118359892

SALP:

111969410

SNH:

120025295

CCLU:

121540915 121548268

ELS:	105016137(cmtr2)

SFM:	108926348(cmtr2)

PKI:	111851836(cmtr2)

AANG:

118226693(cmtr2)

LOC:	102682467(cmtr2)

PSPA:

121296043(cmtr2)

ARUT:

117424351(cmtr2)

PSEX:

120535311(cmtr2)

LCM:	102351997(CMTR2)

RTP:	109917875(cmtr2)

CPLA:

122558597(cmtr2)

HOC:	132824040(cmtr2)

LERI:

129705248(cmtr2)

PMRN:

116945754(CMTR2)

LRJ:	133339537(CMTR2)

BFO:

118422002

BBEL:

109461949

CIN:

100183192

SCLV:

120327729

SPU:

579852

APLC:

110981401

SKO:

102810133

DME:	Dmel_CG5032(aft)

DER:

6546823

DSE:

6609695

DSI:	Dsimw501_GD11317(Dsim_GD11317)

DYA:	Dyak_GE11900

DSR:

110177913

DPO:

4805375

DPE:

6593041

DMN:

108159493

DWI:

6643365

DGR:

6560499

DMO:	Dmoj_GI19115

DAZ:

108616474

DNV:

108654171

DHE:

111605360

DVI:

6624811

CCAT:

101461463

BOD:

106621703

BDR:

105227722

RZE:

108374497

AOQ:

129244832

TDA:	119679677 119679772

MDE:

101891303

SCAC:

106085446

LCQ:

111684792

LSQ:	119611351 119612357

GFS:	119643972 119643976

ECOE:

129952044

CLON:

129615715

AGA:

1276114

ACOZ:

120948780

AARA:

120893572

AMER:

121592562

ASTE:

118510067

AFUN:

125767877

AMOU:

128304302

AALI:

118463025

AAG:

5568314

AALB:

109427326 115257149

CQU:	CpipJ_CPIJ011919 CpipJ_CPIJ011921

CPII:

120430016

BCOO:

119076033 119079247

AME:

113218670

ACER:

108001937

BIM:

100743301

BBIF:

117206100

BVK:

117235514

BVAN:

117154031

BTER:

105667128

BAFF:

126916827

BPYO:

122565614

BPAS:

132905133

FVI:

122532494

CCAL:

108632965

OBB:

114874959

OLG:

117605185

MGEN:

117218672

CGIG:

122398426 122403129

AEC:

105150713

ACEP:

105619279

PBAR:

105433397

VEM:

105570574

CFO:

112637055

FEX:

115242902

LHU:

105673251

PGC:

109852588

OBO:

105280253

PCF:

106793804

PFUC:

122520258

VPS:

122632000

VCRB:

124426936

VVE:

124952573

CSOL:

105368692

TPRE:

106656717

LBD:

127277124

MDL:

103571661

CGLO:

123273043

FAS:

105264276

DAM:

114841217

CCIN:

107269744

DSM:

124407504

NPT:

124215652

NFB:

124177328

NVG:

124299980

AROA:

105684427

TCA:

658920

DPA:

109542469

SOY:

115878458

CSET:

123308455

AGB:

108911021

NVL:

108559500

APLN:

108738529 112905565

PPYR:

116162328

OTU:

111428329

BMOR:

101737449

BMAN:

114242626

MSEX:

115448380

BANY:

112046744

MJU:

123864879

NIQ:

126778038

VCD:

124538739

MCIX:

123662513

PMAC:

106708107

PPOT:

106108117

PXU:

106117424

PRAP:

110991846

PBX:

123713396

PNAP:

125063645

ZCE:

119830232

CCRC:

123701454

LSIN:

126964501

AAGE:

121729716

HAW:

110374672

HZE:

124642705

TNL:

113493669

SLIU:

111351389

OFU:

114353569

PXY:

105391860

PGW:

126382211

CFEL:

113386834

API:	100161495 100166993

DNX:	107164413 107166418

AGS:	114130006 114132831

RMD:	113551785 113554572

ACOO:

126836140 126838038

DVT:	126895162 126895784 126896031 126901411 126902088

BTAB:

109036652

DCI:

103518328

CLEC:

106662807

HHAL:

106687131

NLU:

111051263

HVI:

124360874

MQU:

128991662

PHU:	Phum_PHUM357230

FOC:

113215215

TPAL:

117641262

ZNE:

110838990

CSEC:

111867858

BROR:

134533058

IEL:

124159108

FCD:	110846416 110849257

DPX:	DAPPUDRAFT_217428

DMK:

116923536

DPZ:

124325902

PVM:

113807020

PJA:

122267989

PCHN:

125037317

HAME:

121872879

PCLA:

123768166

CQD:

128700768

PTRU:

123515459

ESN:

127002105

MNZ:

135202343

HAZT:

108678175

EAF:

111701188

LSM:

121132215

TCF:

131887752

PPOI:

119107156 119108251

DSV:

119453239

RSAN:

119399938

RMP:	119179284 119185607

VDE:

111250964

VJA:

111260627

TUT:

107366167

DFR:

124496094

CSCU:

111634108 111634535

PTEP:

107447964

ABRU:

129985230

UDV:

129224835

SDM:

118186329

LPOL:

106462714

CEL:	CELE_Y40D12A.1(cmtr-2)

CBR:

CBG_15196

NAI:	NECAME_12065

BMY:	BM_BM6507(Bm6507)

LOA:	LOAG_09900

HRO:	HELRODRAFT_150382

LGI:	LOTGIDRAFT_139267

PCAN:

112575293

BGT:

106063510

GAE:

121383838

HRF:

124141798

HRJ:

124269824

CRG:

105322668

CVN:

111126588

CANU:

128155405

OED:

125680968

MYI:

110451340

PMAX:

117321994

MMER:

123553422

RPHI:

132719928 132727036

OBI:

106867681

OSN:

115225780

LAK:

106171494

NVE:

5516727

EPA:

110239898

ATEN:

116304606

ADF:	107352032 107352033

AMIL:

114975732

PDAM:

113669963

SPIS:

111324537

DGT:

114533580

XEN:

124439144

HMG:

101238854

HSY:

130641681

TAD:	TRIADDRAFT_53500

AQU:

105313501

VCN:	VOLCADRAFT_62343(ssa18)

MBR:	MONBRDRAFT_26377 MONBRDRAFT_31292

TAN:

TA05485

TPV:

TP03_0172

TOT:	TOT_030000677

BEQ:	BEWA_049660

BBO:	BBOV_I003250

BMIC:

BMR1_02g01785

BBIG:

BBBOND_0207410

CPV:

cgd7_2570

CHO:	Chro.70293

TGO:	TGME49_278500

PTM:	GSPATT00024452001

GTT:	GUITHDRAFT_106459 GUITHDRAFT_113944 GUITHDRAFT_43071 GUITHDRAFT_43190 GUITHDRAFT_72655 GUITHDRAFT_75183

TBR:	Tb11.02.2500

TBG:	TbgDal_XI5510

TCR:	507669.180

LMA:	LMJF_24_0110

LIF:	LINJ_24_0110

LDO:	LDBPK_240110

LMI:	LMXM_24_0110

LBZ:	LBRM_24_0110

LPAN:

LPMP_240110

NGR:	NAEGRDRAFT_70140

» show all

Reference

1 [PMID:16516986]

Authors

Arhin GK, Ullu E, Tschudi C

Title

2'-O-methylation of position 2 of the trypanosome spliced leader cap 4 is mediated by a 48 kDa protein related to vaccinia virus VP39.

Journal

Mol Biochem Parasitol 147:137-9 (2006)
DOI:10.1016/j.molbiopara.2006.01.011

Sequence

[tbr:Tb11.02.2500]

Reference

2 [PMID:21310715]

Authors

Werner M, Purta E, Kaminska KH, Cymerman IA, Campbell DA, Mittra B, Zamudio JR, Sturm NR, Jaworski J, Bujnicki JM

Title

2'-O-ribose methylation of cap2 in human: function and evolution in a horizontally mobile family.

Journal

Nucleic Acids Res 39:4756-68 (2011)
DOI:10.1093/nar/gkr038

Sequence

[hsa:55783]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.296

IUBMB Enzyme Nomenclature:

2.1.1.296

ExPASy - ENZYME nomenclature database:

2.1.1.296

BRENDA, the Enzyme Database:

2.1.1.296

DBGET integrated database retrieval system