KEGG   ENZYME: 2.1.1.300
Entry
EC 2.1.1.300                Enzyme                                 
Name
pavine N-methyltransferase;
PavNMT
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:(+/-)-pavine N-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + (+/-)-pavine = S-adenosyl-L-homocysteine + N-methylpavine [RN:R10680]
Reaction(KEGG)
R10680
Substrate
S-adenosyl-L-methionine [CPD:C00019];
(+/-)-pavine [CPD:C20771]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
N-methylpavine [CPD:C20770]
Comment
The enzyme, isolated from the plant Thalictrum flavum, also methylates (R,S)-stylopine and (S)-scoulerine (11%) with lower activity (14% and 11%, respectively).
History
EC 2.1.1.300 created 2014
Orthology
K21458  pavine N-methyltransferase
Reference
1  [PMID:18997344]
  Authors
Jain A, Ziegler J, Liscombe DK, Facchini PJ, Tucker PA, Panjikar S
  Title
Purification, crystallization and X-ray diffraction analysis of pavine N-methyltransferase from Thalictrum flavum.
  Journal
Acta Crystallogr Sect F Struct Biol Cryst Commun 64:1066-9 (2008)
DOI:10.1107/S1744309108033046
Reference
2  [PMID:19624470]
  Authors
Liscombe DK, Ziegler J, Schmidt J, Ammer C, Facchini PJ
  Title
Targeted metabolite and transcript profiling for elucidating enzyme function: isolation of novel N-methyltransferases from three benzylisoquinoline alkaloid-producing species.
  Journal
Plant J 60:729-43 (2009)
DOI:10.1111/j.1365-313X.2009.03980.x
  Sequence
[ag:ACO90251]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.300
IUBMB Enzyme Nomenclature: 2.1.1.300
ExPASy - ENZYME nomenclature database: 2.1.1.300
BRENDA, the Enzyme Database: 2.1.1.300

  All links  
Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (3)   
   KEGG REACTION (1)   
   KEGG RCLASS (2)   
Gene (2)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (1)   
Protein sequence (13)   
   UniProt (2)   
   SWISS-PROT (1)   
   PDBSTR (10)   
3D Structure (5)   
   PDB (5)   
Protein domain (1)   
   InterPro (1)   
All databases (28)   

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