KEGG ENZYME: 2.1.1.327

ENZYME: 2.1.1.327

Entry

EC 2.1.1.327                Enzyme

Name

phenazine-1-carboxylate N-methyltransferase;
phzM (gene name)

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:phenazine-1-carboxylate 5-methyltransferase

Reaction(IUBMB)

S-adenosyl-L-methionine + phenazine-1-carboxylate = S-adenosyl-L-homocysteine + 5-methylphenazine-1-carboxylate [RN:R11533]

Reaction(KEGG)

R11533

Substrate

S-adenosyl-L-methionine [CPD:C00019];
phenazine-1-carboxylate [CPD:C21442]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
5-methylphenazine-1-carboxylate [CPD:C21295]

Comment

The enzyme, characterized from the bacterium Pseudomonas aeruginosa, is involved in the biosynthesis of pyocyanin, a toxin produced and secreted by the organism. The enzyme is active in vitro only in the presence of EC 1.14.13.218, 5-methylphenazine-1-carboxylate 1-monooxygenase.

History

EC 2.1.1.327 created 2016

Pathway

ec00405

Phenazine biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K21103

phenazine-1-carboxylate N-methyltransferase

Genes

PAE:	PA4209(phzM)

PAEV:

N297_4341

PAEI:

N296_4341

PAU:	PA14_09490(phzM)

PAP:	PSPA7_0889

PAG:	PLES_07181(phzM)

PAF:	PAM18_0728(phzM)

PNC:	NCGM2_5454(phzM)

PAEB:

NCGM1900_0750(phzM)

PDK:	PADK2_03625

PSG:	G655_03660

PRP:	M062_22175

PAEP:

PA1S_03775

PAER:

PA1R_gp2100

PAEM:

U769_03805

PAEL:

T223_03710

PAES:

SCV20265_0753

PAEU:

BN889_04683(phzM)

PAEG:

AI22_00135

PAEC:

M802_4339

PAEO:

M801_4207

PPAA:

B7D75_03705

SECH:

B18_04190

SHAW:

CEB94_18915

SCIN:

CP977_15890

SMAO:

CAG99_00495

SKG:	KJK29_05845

ABRY:

NYE86_22030

KBU:	Q4V64_42980

ACTU:

Actkin_02821(tcmN_2)

PRY:	Prubr_64750

» show all

Reference

1 [PMID:17253782]

Authors

Parsons JF, Greenhagen BT, Shi K, Calabrese K, Robinson H, Ladner JE

Title

Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa.

Journal

Biochemistry 46:1821-8 (2007)
DOI:10.1021/bi6024403

Sequence

[pae:PA4209]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.327

IUBMB Enzyme Nomenclature:

2.1.1.327

ExPASy - ENZYME nomenclature database:

2.1.1.327

BRENDA, the Enzyme Database:

2.1.1.327

DBGET integrated database retrieval system