KEGG   ENZYME: 2.1.1.342
Entry
EC 2.1.1.342                Enzyme                                 
Name
anaerobilin synthase;
chuW (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:protoheme C-methyltransferase (anaerobilin-producing)
Reaction(IUBMB)
2 S-adenosyl-L-methionine + protoheme + 2 reduced flavodoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + anaerobilin + Fe2+ + 2 oxidized flavodoxin [RN:R11700]
Reaction(KEGG)
R11700
Substrate
S-adenosyl-L-methionine [CPD:C00019];
protoheme [CPD:C00032];
reduced flavodoxin [CPD:C02745]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
L-methionine [CPD:C00073];
5'-deoxyadenosine [CPD:C05198];
anaerobilin [CPD:C21603];
Fe2+ [CPD:C14818];
oxidized flavodoxin [CPD:C02869]
Comment
The enzyme, studied from the bacterium Escherichia coli O157:H7, is a radical SAM (AdoMet) enzyme that is involved in heme degradation and iron utilization under anaerobic conditions. The enzyme uses two SAM molecules for the reaction. The first molecule is used to generate a 5'-deoxyadenosyl radical, which abstracts a hydrogen atom from the methyl group of the second SAM molecule. The newly formed methylene radical attacks the substrate, causing a rearrangement of the porphyrin ring that results in the liberation of iron.
History
EC 2.1.1.342 created 2017
Orthology
K21936  anaerobilin synthase
Genes
ECE: Z4914(chuW)
ECS: ECs_4383
ECF: ECH74115_4862
ETW: ECSP_4492(chuW)
ELX: CDCO157_4120
ECOO: ECRM13514_4490(chuW)
ECOH: ECRM13516_4290(chuW)
ECG: E2348C_3745(chuW)
EOK: G2583_4238(chuW)
ELR: ECO55CA74_20280
ECP: ECP_3601
ENA: ECNA114_3622(chuW)
ECOS: EC958_3906(chuW)
ECV: APECO1_2946(chuW)
ECM: EcSMS35_3805
ECQ: ECED1_4181
EUM: ECUMN_4003
ECT: ECIAI39_4005
EOC: CE10_4046(chuW)
ECI: UTI89_C4034(chuW)
EIH: ECOK1_3941
ECZ: ECS88_3915
ECC: c4314(chuW)
ELO: EC042_3797(chuW)
ELN: NRG857_17400
ESE: ECSF_3330
EAB: ECABU_c39420
ELU: UM146_17670
ELC: i14_3982(chuW)
ELD: i02_3982(chuW)
ELF: LF82_543
ECOI: ECOPMV1_03832(hemN_2)
ECOJ: P423_19505
EAL: EAKF1_ch2447c
EMA: C1192_15470
ESZ: FEM44_08635
ERUY: OSH18_11670(hutW)
SDZ: Asd1617_04688
KMI: VW41_01195
REE: electrica_03842(hemN_2)
RTG: NCTC13098_05695(hemN_2)
CKO: CKO_02656
CBRA: A6J81_10720
CYO: CD187_17800
CPOT: FOB25_05285
CFQ: C2U38_06080
CIF: AL515_07175
CPAR: CUC49_02260
CITZ: E4Z61_11075
CIB: HF677_017420
CNT: JT31_10585
CEM: LH23_07140
CEN: LH86_07090
CLAP: NCTC11466_04200(hemN_3)
KIE: NCTC12125_00070(hemN_1)
KAS: KATP_10430
KCY: RIN60_05350(hutW)
SYM: K6K13_13825(hutW)
YRE: HEC60_09655
PSHI: SAMEA2665130_1272(hemN_1)
PSGC: G163CM_23440(chuW)
EBF: D782_3336
EBB: F652_1217
YPE: YPO0286
YPK: y0547
YPH: YPC_0718
YPA: YPA_3995
YPN: YPN_3380
YPP: YPDSF_3683
YPG: YpAngola_A0654
YPZ: YPZ3_0247
YPT: A1122_03695
YPD: YPD4_0251
YPX: YPD8_0254
YPW: CH59_1575(hutW)
YPJ: CH55_3474(hutW)
YPV: BZ15_3283(hutW)
YPL: CH46_625(hutW)
YPS: YPTB0344
YPO: BZ17_2224(hutW)
YPI: YpsIP31758_3797
YPY: YPK_3885
YPB: YPTS_0368
YPQ: DJ40_2064(hutW)
YPU: BZ21_3716(hutW)
YPR: BZ20_1767(hutW)
YPC: BZ23_3986(hutW)
YPF: BZ19_3816(hutW)
YEN: YE0335(hemN)
YEP: YE105_C0363
YEY: Y11_35751
YEL: LC20_04852
YEW: CH47_3139(hutW)
YET: CH48_1255(hutW)
YEF: FORC2_3744
YEE: YE5303_30551(hemN)
YSI: BF17_09835
YRO: CH64_2957(hutW)
YAK: ACZ76_10865
YMA: DA391_20705
YHI: D5F51_01930
YCA: F0T03_01695
YMO: HRD69_07440
YAS: N0H69_01760(hutW)
SFW: WN53_23055
SFG: AV650_18870
SFO: Z042_10400
RAH: Rahaq_4850
RACE: JHW33_24980
RAA: Q7S_24786
ROX: BV494_22850
RVC: J9880_23930(hutW)
RBON: QNM34_08490(hutW)
GQU: AWC35_15525 AWC35_18220
ECA: ECA3009
PATR: EV46_14875
PATO: GZ59_30160
PCT: PC1_2750
PCC: PCC21_028180
PCV: BCS7_14055
PWA: Pecwa_1519
PPAR: A8F97_11170
PEC: W5S_1404
PWS: A7983_00760
PPOA: BJK05_08985
PBRA: B5S52_07750
PPUJ: E2566_14385
PARI: I2D83_07655
PAQU: DMB82_0007525(hutW)
PVZ: OA04_30000(hutW)
PPAV: LOZ86_07865(hutW)
PQU: IG609_006620
PPOO: LW347_14090(hutW)
PCAC: OI450_11740(hutW)
PACB: M9782_16380(hutW)
BGJ: AWC36_03355
BRB: EH207_11640
BNG: EH206_07135
BIZ: HC231_07370
LBQ: CKQ53_04110
SLIG: GTU79_12850(hutW)
ETP: LU633_22490(hutW)
KLN: LH22_20160
MINT: C7M51_02699
PVG: CRN77_12965
PHAU: PH4a_15265
PROT: BTA34_07515
PCOL: F1325_09310
PCIB: F9282_09325
PSX: DR96_3161(hutW)
PHEI: NCTC12003_00021(hemN_1)
EIC: NT01EI_1913
ETR: ETAE_1793(hemN1)
ETD: ETAF_1620(hutW)
ETE: ETEE_3837(hemN)
ETC: ETAC_08230
EDL: AAZ33_09175(hutW)
EHO: A9798_08465
HAV: AT03_10710
HPAR: AL518_15120
OPO: DSM2777_16765
PRAG: EKN56_03515
LRI: NCTC12151_01770(hemN_3)
HDU: HD_0037(chuW)
GLE: CJD39_09425
MHT: D648_1760
MHQ: D650_26390
MHAT: B824_24850
MHX: MHH_c07140(hutW)
MHAE: F382_07265
MHAM: J450_07765
MHAO: J451_08700
MHAL: N220_00820
MHAQ: WC39_13245
MHAY: VK67_13250
MVI: X808_20240
MVG: X874_18970
MVE: X875_1040
APL: APL_1523(chuW)
APJ: APJL_1549(chuW)
APA: APP7_1583(chuW)
ASI: ASU2_10055
ASS: ASU1_10100
AEU: ACEE_10350
ALIG: NCTC10568_01864(hemN_2)
AGP: NYR63_08165(hutW)
AGK: NYR60_08320(hutW)
GAN: UMN179_00603
BTO: WQG_6040
BTRE: F542_16010
BTRH: F543_17700
BTRA: F544_6360
VCH: VC_A0909
VCF: IR04_03985
VCS: MS6_A0948
VCE: Vch1786_II0595
VCQ: EN18_02840
VCJ: VCD_000422
VCI: O3Y_17763
VCO: VC0395_0328(hutW)
VCR: VC395_A0934(hutW)
VCM: VCM66_A0869(hutW)
VCL: VCLMA_B0672
VCX: VAA049_2079(hutW)
VCZ: VAB027_1208(hutW)
VVU: VV2_1615
VVY: VVA0425
VVM: VVMO6_03382
VVL: VV93_v1c34110
VPA: VPA0427
VPB: VPBB_A0396
VPK: M636_01405
VPF: M634_24330
VPH: VPUCM_21207
VHA: VIBHAR_05245
VCA: M892_25080
VAG: N646_3082
VEX: VEA_001356
VHR: AL538_26545
VOW: A9237_18670
VRO: BSZ04_06925
VSP: VS_II0290
VFU: vfu_B01288
VNI: VIBNI_A2348(phuW)
VCY: IX92_22195
VCT: JV59_17400
VTU: IX91_17895
VEU: IXK98_01645
VSI: MTO69_15560(hutW)
VMI: AL543_00430
VBR: A6E01_14505
VSC: VSVS12_01181
VSH: BSZ05_24720
VTA: B1790
VAF: D1115_20610
VNL: D3H41_24440
VCC: FAZ90_19785(hutW)
VAS: GT360_09280
VZI: G5S32_12455
VNV: IF132_02695
VGI: MID13_17295(hutW)
VSL: LTQ54_18765(hutW)
VPL: SA104470976_03628(hutW)
VTI: CEQ48_03630
VCRA: IS519_19095
VLE: ISX51_16875(hutW)
VSY: K08M4_32660(hemN_3)
VMT: QYQ96_17070(hutW)
VFI: VF_1226(hutW)
VFM: VFMJ11_1306
VSA: VSAL_I1750(phuW)
AWD: AWOD_I_1443(phuW)
PPR: PBPRA2102(Y0547)
PGB: H744_2c2296
PDS: CAY62_05885
PGH: FH974_09230
GHO: AL542_02035(hutW)
GKD: K6Q96_20445(hutW)
SALY: E8E00_06055
SCOT: HBA18_07640
SPRO: N7E60_07780(hutW)
PCI: PCH70_51090
PCG: AXG94_10560
PASI: LG197_14700
EMO: DM558_07035
SPL: Spea_3339
SHL: Shal_3411
SWP: swp_3985
SVO: SVI_3565
SJA: SJ2017_0135
SPSW: Sps_04905
SALG: BS332_21045
SEUR: FM038_25965(hutW)
AGZ: M0C34_06620(hutW)
AGQ: LQZ07_16820(hutW)
FBL: Fbal_0997 Fbal_3339
FES: HER31_04505
MVS: MVIS_2107(phuW)
ALV: Alvin_1684
HHA: Hhal_0384
HHC: M911_15930
EBS: ECTOBSL9_2338
RFO: REIFOR_01945
ENW: MJO57_27735(hutW)
EEI: NX720_12680(hutW)
ENG: O2T12_02330(hutW)
EMP: EZMO1_4721(hemN3)
AHA: AHA_1982
AHY: AHML_13035
AHD: AI20_07140
AHR: V428_13490
AHP: V429_13510
AHJ: V469_09610
AHH: RY45_12760
AHI: VU14_09540
AAJ: BOQ57_12580
ASA: ASA_1862(hugW)
AEO: O23A_p1153
AVR: B565_2249
AVO: AMS64_11005
AMED: B224_2619
ASR: WL1483_3905
ADH: CK627_13735
ACAV: VI35_09760
AEM: CK911_08285
AEA: C2U39_09635
ARV: C7N77_15435
AES: C2U30_04090
AEL: NCTC12917_01871(hugW)
AALL: I6G90_06845
AJD: I6H43_20170
ARIV: KYK33_10530(hutW)
AEJ: E5E97_17540
ABES: IU367_12355
GAP: GAPWK_1068
GAQ: RAM11_08055(hutW)
FPP: FPB0191_00913
NEL: NELON_08055
NSI: A6J88_03965
NMJ: NM96_12135
NEK: CGZ77_10595
NFV: FAH67_10645
NSF: FAH66_04115
NBL: GJV52_04285
NANI: NCTC12227_01725(hemN_3)
NBC: H3L91_09255
NSC: J7445_10855
NPF: LPB400_00330(hutW)
NMB: MON40_11540(hutW)
SALV: SALWKB2_1968
KKI: KKKWG1_0582(chuW)
ECOR: SAMEA4412678_0282(hemN_1)
EEX: EZJ17_07035
MCER: MON37_00250(hutW)
IOD: EJO50_01135
JEU: BJP62_00550
JES: JHS3_07260
LHK: LHK_01195(chuW)
BTE: BTH_II0299
BTQ: BTQ_3593
BTJ: BTJ_4629
BTZ: BTL_5417
BTD: BTI_4964
BTV: BTHA_4786
BTHE: BTN_5087
BTHM: BTRA_5355
BTHA: DR62_5217
BTHL: BG87_5423
BUU: WS70_27625
BHG: I6G56_28210
BUD: AQ610_30660
BUL: BW21_4493
PTRO: G5S35_31695
JAB: VN23_01425
OFO: BRW83_1102(hemN_3)
SUTK: FG381_10915 FG381_10980
SWS: I6J16_02115 I6J16_02120
BBAY: A4V04_09395
CHEA: PVE73_21175(hutW)
RPA: TX73_010940(hutW)
RPB: RPB_0164
RPC: RPC_1971
RPD: RPD_2352
RPE: RPE_3151
RPT: Rpal_2413
RPX: Rpdx1_3405
HDN: Hden_0599
HNI: W911_02640
RVA: Rvan_2515
RLAC: QMO75_14775(hutW)
BVR: BVIR_1164
BLAG: BLTE_15640(hemN_1)
PSF: PSE_0684(hemN)
RCP: RCAP_rcc00151(hemN1)
RBL: B6K69_04610
PVER: E3U25_24300 E3U25_24675
RSU: NHU_00565
RHM: B5V46_15110
RCE: RC1_3842(hemN)
RPM: RSPPHO_02607(hemN)
CCV: CCV52592_0647
CGRA: CGRAC_0445
CURE: CUREO_0226
CSPF: CSF_0147
CPIN: CPIN18020_0552
CRX: CRECT_0675
CGEO: CGEO_1817
CBLA: CBLAS_1662
CMUC: CMCT_1442
CSHO: CSHOW_1424
DOA: AXF15_02795
DOG: HP555_07135
DOV: DSCO28_24980
BBAE: FRD01_15005
BXI: BK049_16365
BALT: CFN77_11320
BKO: CKF48_23135
BHUI: LOK74_12225
TUM: CBW65_12540 CBW65_12545
TAB: CIG75_15975 CIG75_15980
CBE: Cbei_0683
CBZ: Cbs_0683
SGY: Sgly_1484
ACEL: acsn021_02700
DLO: K5I24_13355
KPAR: JL105_07965
CAZO: G3A45_11385
VPR: Vpar_1337
VAT: B7L28_06765
VRM: 44547418_01417(hemN_2)
VDN: NCTC11831_00486(hemN_1)
VNK: VEIT17_04790
VRG: OKW85_02595(hutW)
MHW: ACT01_05170
MEG: DKB62_01995
MMAX: LCQ47_11455(hutW)
DPN: BCB69_06120
DHO: Dia5BBH33_18270
SSG: Selsp_1717
SRI: SELR_11700
SELE: ADJ74_05930
SELO: AXE86_00725 AXE86_03655
SELT: BCS37_01770
SDIA: QU667_01980(hutW)
MHG: MHY_26410
MFUN: GXM21_08215(hutW)
SGBI: P3F81_02360(hutW) P3F81_12715(hutW)
PFT: JBW_01206
STED: SPTER_02410 SPTER_41200
AIN: Acin_0814(hemN)
PFAC: PFJ30894_00785(hemN_2)
SDV: BN159_2009
KBS: EPA93_15615
OBT: OPIT5_19630
RUFI: K0V07_11075(hutW)
VBS: EGM51_13195
PIR: VN12_25605(hemN_3)
PBU: L21SP3_01678(hemN_2)
VBC: C5Q97_14580
TPED: TPE_1646
SSM: Spirs_1131
SLR: L21SP2_0606
FVA: FV113G1_19070
FUL: C4N20_14530 C4N20_14555
FMO: C4N19_06045
LEO: AMK43_02430
LHF: JCM16775_0691
LHG: JMUB5056_0559
LTE: JMUB4039_1845
LGO: JCM16774_1764
STR: Sterm_0588
FAX: FUAX_08670(hemN_1)
TLE: Tlet_1247
DIN: Selin_2025
DAP: Dacet_0823
GTL: EP073_08435(hutW)
TEU: TEU_08400
TIC: FH039_06835
 » show all
Reference
1  [PMID:27791000]
  Authors
LaMattina JW, Nix DB, Lanzilotta WN
  Title
Radical new paradigm for heme degradation in Escherichia coli O157:H7.
  Journal
Proc Natl Acad Sci U S A 113:12138-12143 (2016)
DOI:10.1073/pnas.1603209113
Reference
2  [PMID:28045510]
  Authors
LaMattina JW, Delrossi M, Uy KG, Keul ND, Nix DB, Neelam AR, Lanzilotta WN
  Title
Anaerobic Heme Degradation: ChuY Is an Anaerobilin Reductase That Exhibits Kinetic Cooperativity.
  Journal
Biochemistry 56:845-855 (2017)
DOI:10.1021/acs.biochem.6b01099
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.342
IUBMB Enzyme Nomenclature: 2.1.1.342
ExPASy - ENZYME nomenclature database: 2.1.1.342
BRENDA, the Enzyme Database: 2.1.1.342

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Chemical substance (9)   
   KEGG COMPOUND (9)   
Chemical reaction (1)   
   KEGG REACTION (1)   
Gene (4005)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (469)   
   KEGG MGENES (3313)   
   RefGene (222)   
Protein sequence (3)   
   UniProt (2)   
   SWISS-PROT (1)   
DNA sequence (19)   
   GenBank (11)   
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Protein domain (5)   
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