KEGG   ENZYME: 2.1.1.343
Entry
EC 2.1.1.343                Enzyme                                 
Name
8-amino-8-demethylriboflavin N,N-dimethyltransferase;
rosA (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:8-amino-8-demethylriboflavin N,N-dimethyltransferase
Reaction(IUBMB)
2 S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = 2 S-adenosyl-L-homocysteine + roseoflavin (overall reaction) [RN:R11778];
(1a) S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = S-adenosyl-L-homocysteine + 8-demethyl-8-(methylamino)riboflavin [RN:R11779];
(1b) S-adenosyl-L-methionine + 8-demethyl-8-(methylamino)riboflavin = S-adenosyl-L-homocysteine + roseoflavin [RN:R11780]
Reaction(KEGG)
R11778 R11779 R11780
Substrate
S-adenosyl-L-methionine [CPD:C00019];
8-amino-8-demethylriboflavin [CPD:C21646];
8-demethyl-8-(methylamino)riboflavin [CPD:C21648]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
roseoflavin [CPD:C21647];
8-demethyl-8-(methylamino)riboflavin [CPD:C21648]
Comment
The enzyme, characterized from the soil bacterium Streptomyces davawensis, catalyses the last two steps in the biosynthesis of the antibiotic roseoflavin.
History
EC 2.1.1.343 created 2017
Pathway
ec00998  Biosynthesis of various antibiotics
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K22269  8-amino-8-demethylriboflavin N,N-dimethyltransferase
Genes
SDV: BN159_8032(rosA)
SCIR: STRCI_000638(rosA)
Reference
1  [PMID:21911488]
  Authors
Jankowitsch F, Kuhm C, Kellner R, Kalinowski J, Pelzer S, Macheroux P, Mack M
  Title
A novel N,N-8-amino-8-demethyl-D-riboflavin Dimethyltransferase (RosA) catalyzing the two terminal steps of roseoflavin biosynthesis in Streptomyces davawensis.
  Journal
J Biol Chem 286:38275-85 (2011)
DOI:10.1074/jbc.M111.292300
  Sequence
[sdv:BN159_8032]
Reference
2  [PMID:26913589]
  Authors
Tongsook C, Uhl MK, Jankowitsch F, Mack M, Gruber K, Macheroux P
  Title
Structural and kinetic studies on RosA, the enzyme catalysing the methylation of 8-demethyl-8-amino-d-riboflavin to the antibiotic roseoflavin.
  Journal
FEBS J 283:1531-49 (2016)
DOI:10.1111/febs.13690
  Sequence
[sdv:BN159_8032]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.343
IUBMB Enzyme Nomenclature: 2.1.1.343
ExPASy - ENZYME nomenclature database: 2.1.1.343
BRENDA, the Enzyme Database: 2.1.1.343

  All links  
Pathway (7)   
   KEGG PATHWAY (6)   
   KEGG MODULE (1)   
Chemical substance (5)   
   KEGG COMPOUND (5)   
Chemical reaction (7)   
   KEGG REACTION (3)   
   KEGG RCLASS (4)   
Gene (3)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (2)   
Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
Protein domain (5)   
   InterPro (5)   
All databases (29)   

Download RDF
DBGET integrated database retrieval system