Entry
Name
menaquinone C8-methyltransferase;
mqnK (gene name);
menK (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:menaquinone C8-methyltransferase
Reaction(IUBMB)
(1) 2 S-adenosyl-L-methionine + a menaquinone + reduced flavodoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + an 8-methylmenaquinone + oxidized flavodoxin [RN:
R12238 ];
(2) 2 S-adenosyl-L-methionine + a 2-demethylmenaquinone + reduced flavodoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + a 2-demethyl-8-methylmenaquinone + oxidized flavodoxin [RN:
R12239 ]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:
C00019 ];
menaquinone [CPD:
C00828 ];
reduced flavodoxin [CPD:
C02745 ];
2-demethylmenaquinone [CPD:
C05818 ]
Product
S-adenosyl-L-homocysteine [CPD:
C00021 ];
L-methionine [CPD:
C00073 ];
5'-deoxyadenosine [CPD:
C05198 ];
8-methylmenaquinone [CPD:
C22027 ];
oxidized flavodoxin [CPD:
C02869 ];
2-demethyl-8-methylmenaquinone [CPD:
C22028 ]
Comment
The enzyme, found in a wide range of bacteria and archaea, is a radical SAM (AdoMet) enzyme that utilizes two molecules of S-adenosyl-L-methionine, one as the methyl group donor, and one for the creation of a 5'-deoxyadenosine radical that drives the reaction forward.
History
EC 2.1.1.350 created 2018
Orthology
K23078 menaquinone C8-methyltransferase
Genes
SJA : SJ2017_2848 SJ2017_4065
SLJ : EGC82_01525 EGC82_12775
SMAI : EXU30_07885 EXU30_09715
SPOL : FH971_00395 FH971_03420 FH971_08795
SBK : SHEWBE_0429 SHEWBE_1745
SPSH : FM037_07180 FM037_27360
SEUR : FM038_006715 FM038_024580
SGLA : FJ709_00365 FJ709_12595
HPUL : NCTC13154_00279(hemN_1)
CJV : MTVDSCj20_0334(hemN1)
CFT : CFF04554_1668(hemN3)
CFV : CFVI03293_1690(hemN3)
CFX : CFV97608_1793(hemN3)
CAMP : CFT03427_1618(hemN3)
CCV : CCV52592_1538(hemN1)
CCO : CCC13826_1654(hemN1)
CCOC : CCON33237_0218(hemN1)
CLR : UPTC16701_1531(hemN3)
CLM : UPTC16712_1565(hemN3)
CLQ : UPTC4110_1538(hemN3)
CLN : UPTC3659_1773(hemN3)
CCOO : ATE51_03488(hemN_3)
CAJ : CIG1485E_1563(hemN3)
CAMR : CAQ16704_1568(hemN3)
CSM : CSUB8521_1773(hemN3)
CSF : CSUB8523_1875(hemN3)
CHV : CHELV3228_1122(hemN1)
CPIN : CPIN18020_0428(hemN1)
CAMZ : CVIC12175_1403(hemN3)
CNV : CNZW441b_1322(hemN3)
HEBR : AEBR_1183(hemN1) AEBR_2109(hemN3)
MANA : MAMMFC1_00559(hemN_1)
CCU : Ccur_00440 Ccur_11490
EGD : GS424_000175 GS424_009750
AHAT : ADCFC_01500 ADCFC_13290
CBAC : JI75_00160 JI75_05040
BWA : HLV38_01340 HLV38_05190
RTS : CE91St31_06080 CE91St31_32630
EBZ : J7S26_08075 J7S26_08425
» show all
Taxonomy
Reference
Authors
Hein S, Klimmek O, Polly M, Kern M, Simon J
Title
A class C radical S-adenosylmethionine methyltransferase synthesizes 8-methylmenaquinone.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 2.1.1.350