KEGG ENZYME: 2.1.1.366

ENZYME: 2.1.1.366

Entry

EC 2.1.1.366                Enzyme

Name

[histone H3]-N6,N6-dimethyl-lysine9 N-methyltransferase;
KMT1E (gene name);
SETDB1 (gene name);
KIAA0067 (gene name)

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:[histone H3]-N6,N6-dimethyl-L-lysine9 N6-methyltransferase

Reaction(IUBMB)

S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine9 = S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine9

Reaction(KEGG)

(other) R04867

Substrate

S-adenosyl-L-methionine [CPD:C00019];
[histone H3]-N6,N6-dimethyl-L-lysine9

Product

S-adenosyl-L-homocysteine [CPD:C00021];
[histone H3]-N6,N6,N6-trimethyl-L-lysine9

Comment

The enzyme methylates only dimethylated lysine9 of histone H3 (H3K9), forming the trimethylated form. This modification influences the binding of chromatin-associated proteins. In general, the methylation of H3K9 leads to transcriptional repression of the affected target genes. The enzyme is highly upregulated in Huntington disease patients. cf. EC 2.1.1.367, [histone H3]-lysine9 N-methyltransferase, and EC 2.1.1.368, [histone H3]-lysine9 N-dimethyltransferase, and EC 2.1.1.355, [histone H3]-lysine9 N-trimethyltransferase.

History

EC 2.1.1.366 created 2020

Pathway

ec00310

Lysine degradation

ec01100

Metabolic pathways

Orthology

K11421

[histone H3]-N6,N6-dimethyl-lysine9 N-methyltransferase

Genes

HSA:	9869(SETDB1)

PTR:	469481(SETDB1)

PPS:	100972839(SETDB1)

GGO:	101146215(SETDB1)

PON:	100431310(SETDB1)

NLE:	100581330(SETDB1)

HMH:	116469470(SETDB1)

MCC:	716141(SETDB1)

MCF:	102117410(SETDB1)

MTHB:

126931370

MNI:	105497821(SETDB1)

CSAB:

103247223(SETDB1)

CATY:

105590302(SETDB1)

PANU:

101002056(SETDB1)

TGE:	112620272(SETDB1)

MLEU:

105534755(SETDB1)

RRO:	104681064(SETDB1)

RBB:	108533259(SETDB1)

TFN:	117075079(SETDB1)

PTEH:

111543630(SETDB1)

CANG:

105508932(SETDB1)

CJC:	100411734(SETDB1)

SBQ:	101042658(SETDB1)

CIMI:

108316459(SETDB1)

MMUR:

105872992(SETDB1)

LCAT:

123633944(SETDB1)

PCOQ:

105821953(SETDB1)

OGA:	100940994(SETDB1)

MMU:	84505(Setdb1)

MCAL:

110291522(Setdb1)

MPAH:

110320737(Setdb1)

RNO:	689883(Setdb1)

MCOC:

116093409(Setdb1)

ANU:	117706616(Setdb1)

MUN:	110560774(Setdb1)

CGE:	100765697(Setdb1)

MAUA:

101843804(Setdb1)

PROB:

127232822(Setdb1)

PLEU:

114682368(Setdb1)

MORG:

121437839 121450501

MFOT:

126489995

AAMP:

119800749(Setdb1)

NGI:	103730089(Setdb1)

HGL:	101715248(Setdb1)

CPOC:

100721293(Setdb1)

CCAN:

109681205(Setdb1)

DORD:

105984728(Setdb1)

DSP:	122109123(Setdb1)

PLOP:

125360015(Setdb1)

NCAR:

124959679

MMMA:

107156826(Setdb1)

OCU:

100355877

OPI:	101525521(SETDB1)

TUP:	102502264(SETDB1)

GVR:	103600244(SETDB1)

CFA:	483186(SETDB1)

CLUD:

112663993(SETDB1)

VVP:	112907769(SETDB1)

VLG:	121490848(SETDB1)

NPO:	129501338(SETDB1)

AML:	100482250(SETDB1)

UMR:	103667532(SETDB1)

UAH:	113246155(SETDB1)

UAR:	123787733(SETDB1)

ELK:

111162065

LLV:

125097118

MPUF:

101677094(SETDB1)

MNP:	132001451(SETDB1)

MLK:	131808783(SETDB1)

NVS:	122900483(SETDB1)

ORO:	101372094(SETDB1)

EJU:	114197337(SETDB1)

ZCA:	113925244(SETDB1)

MLX:	118000316(SETDB1)

NSU:	110578595(SETDB1)

LWW:	102732334(SETDB1)

FCA:	101094118(SETDB1)

PCOO:

112871086(SETDB1)

PBG:	122481136(SETDB1)

PVIV:

125159475(SETDB1)

LRUF:

124514448

PTG:	102970727(SETDB1)

PPAD:

109257028(SETDB1)

PUC:

125912118

AJU:

106979835

HHV:	120241290(SETDB1)

BTA:	100138100(SETDB1)

BOM:	102288279(SETDB1)

BIU:	109555305(SETDB1)

BBUB:

102408652(SETDB1)

BBIS:

104987649(SETDB1)

CHX:	102180982(SETDB1)

OAS:	101109230(SETDB1)

BTAX:

128044362(SETDB1)

ODA:	120855906(SETDB1)

CCAD:

122434373(SETDB1)

MBEZ:

129540648(SETDB1)

SSC:	100157920(SETDB1)

CFR:	102512057(SETDB1)

CBAI:

105071738(SETDB1)

CDK:	105103196(SETDB1)

VPC:	102545931(SETDB1)

BACU:

103009894(SETDB1)

BMUS:

118897508(SETDB1)

LVE:	103076461(SETDB1)

OOR:	101270688(SETDB1)

DLE:	111169744(SETDB1)

PSIU:

116751422(SETDB1)

NASI:

112411620(SETDB1)

ECB:	100059254(SETDB1)

EPZ:	103547642(SETDB1)

EAI:	106846522(SETDB1)

MYB:	102257713(SETDB1)

MYD:	102773684(SETDB1)

MMYO:

118672742(SETDB1)

MLF:	102441225(SETDB1)

PKL:	118727467(SETDB1)

EFUS:

103298980(SETDB1)

MNA:	107539129(SETDB1)

DRO:	112313537(SETDB1)

AJM:	119034734(SETDB1)

PDIC:

114488934(SETDB1)

PHAS:

123804304(SETDB1)

MMF:	118637044(SETDB1)

PPAM:

129062305(SETDB1)

HAI:	109372277(SETDB1)

RFQ:	117014537(SETDB1)

PGIG:

120585218(SETDB1)

PVP:	105303858(SETDB1)

RAY:	107499036(SETDB1)

MJV:	108409453(SETDB1)

TOD:	119237184(SETDB1)

SARA:

101554078(SETDB1)

LAV:	100673374(SETDB1)

TMU:

101349341

ETF:	101658224(SETDB1)

DNM:	101437951(SETDB1)

MDO:	100618580(SETDB1)

GAS:	123244280(SETDB1)

SHR:	100928904(SETDB1)

AFZ:

127559091

PCW:	110220597(SETDB1)

OAA:	100091318(SETDB1)

GGA:	100859816(SETDB1)

PCOC:

116238423(SETDB1)

MGP:	100542346(SETDB1)

CJO:	107324577(SETDB1)

TPAI:

128071922(SETDB1)

LMUT:

125685794(SETDB1)

NMEL:

110387900(SETDB1)

APLA:

101794756(SETDB1)

ACYG:

106049268(SETDB1)

CATA:

118260462(SETDB1)

AFUL:

116499648(SETDB1)

TGU:	100231651(SETDB1)

LSR:	110476821(SETDB1)

SCAN:

103824025

PMOA:

120502592(SETDB1)

OTC:	121335607(SETDB1)

PRUF:

121353495(SETDB1)

GFR:	102034546(SETDB1)

OMA:	130263386(SETDB1)

PHI:	102101563(SETDB1)

PMAJ:

107214655(SETDB1)

CCAE:

111939473(SETDB1)

CBRC:

103619600(SETDB1)

ETL:	114071955(SETDB1)

ZAB:	102061470(SETDB1)

SVG:	106862356(SETDB1)

MMEA:

130587054(SETDB1)

HRT:	120764209(SETDB1)

FPG:	101912073(SETDB1)

FCH:	102055212(SETDB1)

CLV:	102094505(SETDB1)

EGZ:	104129700(SETDB1)

NNI:	104014430(SETDB1)

PCRI:

104037271(SETDB1)

PLET:

104619247(SETDB1)

EHS:	104509218(SETDB1)

PCAO:

104051886(SETDB1)

ACUN:

113488725(SETDB1)

TALA:

116964156 122152589

PADL:

103919479(SETDB1)

AFOR:

103900501(SETDB1)

ACHC:

115345534(SETDB1)

HALD:

104312868(SETDB1)

HLE:	104830529(SETDB1)

AGEN:

126040314

GCL:

127027051

CCRI:

104157123(SETDB1)

CMAC:

104487401

MUI:

104548107

BREG:

104638314(SETDB1)

FGA:	104082850(SETDB1)

GSTE:

104254890(SETDB1)

OHA:	104330237(SETDB1)

NNT:

104409719

SHAB:

115602619(SETDB1)

PGUU:

104471117(SETDB1)

ACAR:

104527661(SETDB1)

CPEA:

104390376(SETDB1)

CVF:	104285442(SETDB1)

RTD:	128900884(SETDB1)

CUCA:

104066027(SETDB1)

TEO:	104372742(SETDB1)

AAM:	106495416(SETDB1)

AROW:

112972521(SETDB1)

NPD:	112952968(SETDB1)

TGT:	104573259(SETDB1)

DNE:	112990032(SETDB1)

SCAM:

104139540(SETDB1)

ASN:	102382539(SETDB1)

AMJ:	102575637(SETDB1)

CPOO:

109324826(SETDB1)

CMY:	102937828(SETDB1)

CCAY:

125625560(SETDB1)

DCC:	119848135(SETDB1)

CPIC:

101931963(SETDB1)

TST:	117869706(SETDB1)

CABI:

116817630(SETDB1)

MRV:	120390026(SETDB1)

ACS:	100565799(setdb1)

ASAO:

132764180(SETDB1)

PVT:	110081479(SETDB1)

SUND:

121916245(SETDB1)

PBI:	103053131(SETDB1)

PMUR:

107292899(SETDB1)

CTIG:

120317077(SETDB1)

TSR:	106546292(SETDB1)

PGUT:

117670132(SETDB1)

APRI:

131186537(SETDB1)

PTEX:

113452534 113452537

VKO:	123032928(SETDB1)

PMUA:

114586177(SETDB1)

PRAF:

128403733(SETDB1)

ZVI:	118075630(SETDB1)

HCG:	128337288(SETDB1)

GJA:	107121584(SETDB1)

STOW:

125436664(SETDB1)

EMC:	129342370(SETDB1)

XLA:	108700333(setdb1.S) 432147(setdb1.L)

XTR:	100490309(setdb1)

NPR:	108789810(SETDB1)

RTEM:

120920703(SETDB1)

BBUF:

120981785(SETDB1)

BGAR:

122923222(SETDB1)

MUO:	115458064(SETDB1)

GSH:	117349981(SETDB1)

DRE:	553292(setdb1a) 768131(setdb1b)

SRX:	107717459 107723461 107724106 107739578

SANH:

107656477 107671207 107693347 107700161

SGH:	107573682 107582057 107589406 107599511

CCAR:

109105279 109111939 109111941

CAUA:

113060012 113062169 113077897 113116442 113120007

CGIB:

127935667 127974706 127979020 128030992

PTET:

122360609(setdb1b)

LROH:

127178914(setdb1b) 127182106(setdb1a)

OMC:	131522361(setdb1b) 131525288(setdb1a)

PPRM:

120483677(setdb1a) 120484392(setdb1b)

RKG:	130070813(setdb1a) 130101819(setdb1b)

MAMB:

125243644(setdb1b) 125275542(setdb1a)

CIDE:

127497987 127500631

MASI:

127420967 127425596 127452895 127453831

TROS:

130557672(setdb1b) 130567279(setdb1a)

TDW:	130415319(setdb1a) 130433287(setdb1b)

MANU:

129447933(setdb1b) 129454460(setdb1a)

IPU:	108266106(setdb1b) 108272679(setdb1a)

IFU:	128607541(setdb1b) 128615241(setdb1a)

PHYP:

113535137 113542451(setdb1)

SMEO:

124382145(setdb1a) 124384982(setdb1b)

TFD:	113641276(setdb1a) 113643512(setdb1b)

TVC:	132842876(setdb1a) 132846150(setdb1b)

AMEX:

103027968(setdb1) 103028156

CMAO:

118805978(setdb1a) 118824354(setdb1b)

EEE:	113570608(setdb1) 113572599

CHAR:

105912991(setdb1b)

TRU:	101075200(setdb1)

TFS:

130532902

TNG:	GSTEN00015370G001

LCO:	104920543(setdb1)

NCC:	104944919(setdb1)

TBEN:

117471276 117480563(setdb1b)

CGOB:

115021722

PGEO:

117461095(setdb1b)

GACU:

117541016(setdb1b)

EMAC:

134861592(setdb1b)

ELY:	117266438(setdb1b)

EFO:

125893368

PLEP:

121946021

SLUC:

116049589(setdb1b)

ECRA:

117946699(setdb1b)

ESP:	116691442(setdb1)

PFLV:

114557339

GAT:	120810231(setdb1b)

PPUG:

119198096(setdb1b)

AFB:

129109882

CLUM:

117745225(setdb1b)

PSWI:

130213014(setdb1b)

MSAM:

119897547(setdb1b)

SCHU:

122881587(setdb1b)

CUD:	121514026(setdb1b)

ALAT:

119006504(setdb1b)

MZE:	101465322(setdb1)

ONL:	100710331(setdb1)

OAU:	116321097(setdb1b)

OLA:	101158302(setdb1)

OML:

112143498

CSAI:

133432938(setdb1b)

XMA:	102235762(setdb1)

XCO:	114141956(setdb1)

XHE:	116717442(setdb1)

PRET:

103478723(setdb1)

PFOR:

103136616 103136620

PLAI:

106938375 106943293

PMEI:

106922006(setdb1)

GAF:	122830571(setdb1b)

PPRL:

129371135(setdb1b)

CVG:	107088829(setdb1)

CTUL:

119783009(setdb1b)

GMU:	124866219(setdb1b)

NFU:	107379696(setdb1)

KMR:	108241152(setdb1b)

ALIM:

106515090

NWH:	119410006(setdb1b)

AOCE:

111586482(setdb1)

MCEP:

125016823(setdb1b)

CSEM:

103388279(setdb1)

POV:

109633224

SSEN:

122774888

HHIP:

117777184(setdb1b)

HSP:	118113532(setdb1b)

SMAU:

118299766(setdb1b)

LCF:

108885905

SDU:	111230127(setdb1)

SLAL:

111652197(setdb1)

XGL:	120784269(setdb1b)

HCQ:	109522778(setdb1)

SSCV:

125975637

SBIA:

133501043(setdb1b)

PEE:	133401340(setdb1b)

PTAO:

133479743(setdb1b)

BPEC:

110163046(setdb1)

MALB:

109970226

BSPL:

114842555(setdb1b)

SJO:	128366464(setdb1b)

SASA:

106574954 106588190

STRU:

115173296 115176358 115189463

OTW:	112236082 112247848 112266649

OMY:	110496022 110520820 110536431

OGO:	123998183 124005934 124012669

ONE:	115113782 115123820 115128219

OKI:	109883759 109886460 109907679

OKE:	118374768 118383196 118399124

SALP:

111955709 111964582(setdb1)

SNH:	120022441 120031206 120037512

CCLU:

121543877 121586324 123484099

ELS:	105018987(setdb1) 105030119

SFM:	108923796(setdb1)

PKI:	111853328(setdb1)

AANG:

118225516(setdb1b)

PSPA:

121306955

ARUT:

117395637 117962652

PSEX:

120538027(setdb1a)

LCM:	102356699(SETDB1)

HOC:	132805644(setdb1b)

LERI:

129715984

PMRN:

116939538

LRJ:

133346612

BFO:

118414026

BBEL:

109475626

CIN:

101242243

SCLV:

120329266

SPU:

764341

LPIC:

129268305

APLC:

110986848

SKO:

100372058

DME:	Dmel_CG12196(egg)

DER:

6548401

DSE:

6618876

DSI:	Dsimw501_GD24920(Dsim_GD24920)

DYA:	Dyak_GE11418

DAN:

6494410

DSR:

110189614

DPO:

6898620

DPE:

6592281

DMN:	108159156 117192580

DWI:

6640308

DGR:

6560486

DMO:	Dmoj_GI19513

DAZ:

108615991

DNV:

108654234

DHE:

111593833

DVI:

6626333

CCAT:

101450333

BOD:

106626903

BDR:

105225468

RZE:

108370615

AOQ:

129244009

TDA:	119680913 119682533

MDE:

101893339

SCAC:

106082649

LCQ:

111689074

LSQ:

119601703

GFS:

119641642

ECOE:

129949527

CLON:

129614622

HIS:

119647111

ACOZ:

120955372

AARA:

120903557

AMER:

121597845

ASTE:

118513879

AFUN:

125766226

AMOU:

128303339

AALI:

118467738

AALB:

109429413

CQU:	CpipJ_CPIJ016652

CPII:

120413132

CNS:	116338143 116352420

BCOO:

119079737 119083129

AME:

409098

ACER:

107998869

ALAB:

122720229

ADR:

102672185

AFLR:

100863269

BIM:

100745488

BBIF:

117210113

BVK:

117241892

BVAN:

117157119

BTER:

100647514

BAFF:

126921232

BPYO:

122573667

BPAS:

132912755

FVI:

122531369

CCAL:

108631329

OBB:

114874120

OLG:

117607392

MGEN:

117223965

NMEA:

116430891

CGIG:

122395723

SOC:

105201335

MPHA:

105833491

AEC:

105144993

ACEP:

105620791

PBAR:

105429250

VEM:

105561499

HST:

105186113

DQU:

106745347

CFO:

105256000

FEX:

115233743

LHU:

105670399

PGC:

109857860

OBO:

105284457

PCF:

106790393

PFUC:

122518795

VPS:

122627174

VCRB:

124432543

VVE:

124947055

NVI:

100120014

CSOL:

105360527

TPRE:

106652487

LHT:

122499168

LBD:

127281984

MDL:

103577834

CGLO:

123274316

FAS:

105268216

DAM:

107040220

AGIF:

122848592 122849411

VCAN:

122416190

CCIN:

107264745

DSM:

124409918

NPT:

124217567

NFB:

124181100

NLO:

107217676

NVG:

124303853

AROA:

105690658

TCA:

658295

DPA:

109543261

SOY:

115881509

ATD:

109602736

CSET:

123319875

AGB:

108909991

LDC:

111513341

NVL:

108566549

APLN:

108744087

PPYR:

116161259

OTU:

111417041

BMOR:

101742950

BMAN:

114244119

MSEX:

115449535

BANY:

112051969

VCD:

124541909

MCIX:

123668144

PMAC:

106721248

PPOT:

106107193

PXU:

106124549

PRAP:

110997400 123690471

ZCE:

119838086

LSIN:

126974695

HAW:	110383113(egg)

HZE:

124643704

TNL:

113502290

SLIU:

111354717 111354836

OFU:

114359561

PXY:	105384716 105392435

CCRN:

123299289

API:	100165352 100574903

DNX:	107166873 107167319

AGS:	114120564 114123773

RMD:	113553560 113553623

ACOO:

126843160

DVT:	126893853 126893854 126902782

BTAB:

109030364

DCI:

103505795

CLEC:

106668530 106670703

HHAL:

106679343

NLU:

111059365

HVI:

124365706

MQU:

128991472

PHU:	Phum_PHUM415050

FOC:

113208911

TPAL:

117650092

ZNE:

110837361

CSEC:

111863266

BROR:

134538554

IEL:

124153937

FCD:	110847722 110850679

DPX:	DAPPUDRAFT_303556 DAPPUDRAFT_304216

DMK:	116929683 116932783

DPZ:	124312290 124351093

PJA:

122257422

PCHN:

125026400

PMOO:

119580115

HAME:

121855413

PCLA:

123771664

CQD:

128698001

PTRU:

123508802

ESN:

126983536

HAZT:

108673081

EAF:	111695332 111709742

LSM:

121123621

TCF:

131879963

PPOI:

119104173 119107951 119107955

DSV:	119440663 119450321

RSAN:

119383419

RMP:

119170383

VDE:

111249984

VJA:

111269829

TUT:

107372205

CSCU:

111615256 111617813

PTEP:

107451716

ABRU:

129981000

UDV:

129222062

SDM:

118186516

LPOL:

106458997

CEL:	CELE_R05D3.11(met-2)

CBR:	CBG_18157(Cbr-met-2)

CRQ:	GCK72_010167

BMY:	BM_BM5216(Bma-met-2)

LOA:	LOAG_17898

PVUL:

126821962

PCAN:

112559730

GAE:

121381868

HRF:

124120055

HRJ:

124288313

CRG:

105332598

CVN:

111131506

OED:

125646988

MYI:

110459908

PMAX:

117335866

MMER:

123536313

RPHI:

132726729

OBI:

106873585

OSN:

115213825

LAK:

106167373

SMM:	Smp_150850.1

SHX:	MS3_00010918(SETDB1B)

OVI:	T265_08195

EGL:

EGR_06122

NVE:

5506681

EPA:

110238173

ATEN:

116288332

AMIL:

114974694

PDAM:

113666302

DGT:

114527359

XEN:

124455467

HMG:

100203637

» show all

Reference

1 [PMID:11791185]

Authors

Yang L, Xia L, Wu DY, Wang H, Chansky HA, Schubach WH, Hickstein DD, Zhang Y

Title

Molecular cloning of ESET, a novel histone H3-specific methyltransferase that interacts with ERG transcription factor.

Journal

Oncogene 21:148-52 (2002)
DOI:10.1038/sj.onc.1204998

Sequence

[mmu:84505]

Reference

2 [PMID:14536086]

Authors

Wang H, An W, Cao R, Xia L, Erdjument-Bromage H, Chatton B, Tempst P, Roeder RG, Zhang Y

Title

mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression.

Journal

Mol Cell 12:475-87 (2003)
DOI:10.1016/j.molcel.2003.08.007

Reference

3 [PMID:22939622]

Authors

Pinheiro I, Margueron R, Shukeir N, Eisold M, Fritzsch C, Richter FM, Mittler G, Genoud C, Goyama S, Kurokawa M, Son J, Reinberg D, Lachner M, Jenuwein T

Title

Prdm3 and Prdm16 are H3K9me1 methyltransferases required for mammalian heterochromatin integrity.

Journal

Cell 150:948-60 (2012)
DOI:10.1016/j.cell.2012.06.048

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.366

IUBMB Enzyme Nomenclature:

2.1.1.366

ExPASy - ENZYME nomenclature database:

2.1.1.366

BRENDA, the Enzyme Database:

2.1.1.366

DBGET integrated database retrieval system