KEGG ENZYME: 2.1.1.375

ENZYME: 2.1.1.375

Entry

EC 2.1.1.375                Enzyme

Name

NNS virus cap methyltransferase

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:G(5')pppAACA-[mRNA] N7,2'-O-methyltransferase

Reaction(IUBMB)

2 S-adenosyl-L-methionine + G(5')pppAACA-[mRNA] = 2 S-adenosyl-L-homocysteine + m7G(5')pppAmACA-[mRNA] (overall reaction);
(1a) S-adenosyl-L-methionine + G(5')pppAACA-[mRNA] = S-adenosyl-L-homocysteine + G(5')pppAmACA-[mRNA];
(1b) S-adenosyl-L-methionine + G(5')pppAmACA-[mRNA] = S-adenosyl-L-homocysteine + m7G(5')pppAmACA-[mRNA]

Substrate

S-adenosyl-L-methionine [CPD:C00019];
G(5')pppAACA-[mRNA];
G(5')pppAmACA-[mRNA]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
m7G(5')pppAmACA-[mRNA];
G(5')pppAmACA-[mRNA]

Comment

The enzyme from non-segmented negative strain (NNS) viruses (e.g. rhabdoviruses) catalyses two successive methylations. In higher eukaryotes the two methylations occur in the reverse order and are catalysed by two different enzymes (cf. EC 2.1.1.56, mRNA (guanine-N7)-methyltransferase, and EC 2.1.1.57, methyltransferase cap1) that do not required a specific motif.

History

EC 2.1.1.375 created 2021

Orthology

K19376

Morbillivirus RNA-directed RNA polymerase L

K21316

Lyssavirus RNA-directed RNA polymerase L

K25521

Respirovirus RNA-directed RNA polymerase L

K26131

Henipavirus RNA-directed RNA polymerase L

K26132

Orthorubulavirus/Pararubulavirus RNA-directed RNA polymerase L

K26133

Pneumoviridae RNA-directed RNA polymerase L

K26275

Rhabdoviridae RNA-directed RNA polymerase L

K26276

Novirhabdovirus RNA-directed RNA polymerase L

Reference

1 [PMID:19710136]

Authors

Rahmeh AA, Li J, Kranzusch PJ, Whelan SP.

Title

Ribose 2'-O methylation of the vesicular stomatitis virus mRNA cap precedes and facilitates subsequent guanine-N-7 methylation by the large polymerase protein.

Journal

J Virol 83:11043-50 (2009)
DOI:10.1128/JVI.01426-09

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.375

IUBMB Enzyme Nomenclature:

2.1.1.375

ExPASy - ENZYME nomenclature database:

2.1.1.375

BRENDA, the Enzyme Database:

2.1.1.375

All links

Gene (216)   
   KEGG ORTHOLOGY (8)   
   KEGG GENES (208)   
Protein sequence (4242)   
   UniProt (4164)   
   SWISS-PROT (78)   
Protein domain (14)   
   InterPro (14)   
All databases (4472)   

Download RDF

DBGET integrated database retrieval system