Entry
Name
small RNA 2'-O-methyltransferase;
HENMT1 (gene name);
HEN1 (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:[sRNA]-3'-end ribonucleotide 2'-O-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + an [sRNA]-3'-end ribonucleotide = S-adenosyl-L-homocysteine + an [sRNA]-3'-end 2'-O-methylated ribonucleotide
Substrate
S-adenosyl-L-methionine [CPD:
C00019 ];
[sRNA]-3'-end ribonucleotide
Product
S-adenosyl-L-homocysteine [CPD:
C00021 ];
[sRNA]-3'-end 2'-O-methylated ribonucleotide
Comment
The enzyme adds a 2'-O-methyl group to the ribose of the last nucleotide in several types of small RNAs (sRNAs), protecting the 3'-end of sRNAs from uridylation activity and subsequent degradation.
History
EC 2.1.1.386 created 2022
Orthology
K20798 small RNA 2'-O-methyltransferase
Genes
OPI : 101523145 101530433(HENMT1)
VLG : 121472562 121481005 121486408(HENMT1)
ORO : 101378659(HENMT1) 105757520
EJU : 114199783 114208037(HENMT1)
ZCA : 113920575(HENMT1) 113936762
NSU : 110580614(HENMT1) 110580820
MYD : 102758654(HENMT1) 102773992
AJM : 119049920 119054543(HENMT1)
HAI : 109374301(HENMT1) 109391880
PSS : 102449045 102456236(HENMT1)
GSH : 117346079 117346753 117352495
SANH : 107696160(henmt1) 107698398
MASI : 127410358 127415610
AOCE : 111566239 111574174 111588472
SSEN : 122761756 122781310
DSI : Dsimw501_GD15398(Dsim_GD15398)
ASTE : 118512770 118516359
AALB : 109407983 115254252
CQU : CpipJ_CPIJ004597 CpipJ_CPIJ016924
HAME : 121866489 121874259
PCLA : 123771052 123771473
AQU : 100632519 100635584 100635710 109581380
ATH : AT4G20910(HEN1) AT4G20920
CSAT : 104718246 104722971 104728186 104731463
EUS : EUTSA_v10024326mg EUTSA_v10024347mg
BRP : 103858478 103858486 103858496
BNA : 106375681 106375682 106375683 106438472 106438482
BOE : 106295193 106295343 106306076
RSZ : 108842047 108842048 108842050
GMX : 100785461(HEN1B) 100815299(HEN1A)
LJA : Lj4g3v0286120.1(Lj4g3v0286120.1)
PALZ : 118029481 118035900
SSPL : 121784631 121787672
DOSA : Os07t0164000-01(Os07g0164000)
TAES : 123044943 123048735 123052816 123188691
PVIR : 120659404 120662623
SMO : SELMODRAFT_418472 SELMODRAFT_423789
CCAC : CcaHIS019_0104570(CcaverHIS019_0104570)
ABP : AGABI1DRAFT75365(AGABI1DRAFT_75365)
ABV : AGABI2DRAFT177408(AGABI2DRAFT_177408)
SCM : SCHCO_01107153(SCHCODRAFT_01107153)
» show all
Taxonomy
Reference
Authors
Park W, Li J, Song R, Messing J, Chen X.
Title
CARPEL FACTORY, a Dicer homolog, and HEN1, a novel protein, act in microRNA metabolism in Arabidopsis thaliana.
Journal
Reference
Authors
Yu B, Yang Z, Li J, Minakhina S, Yang M, Padgett RW, Steward R, Chen X.
Title
Methylation as a crucial step in plant microRNA biogenesis.
Journal
Reference
Authors
Kirino Y, Mourelatos Z
Title
2'-O-methyl modification in mouse piRNAs and its methylase.
Journal
Reference
Authors
Huang Y, Ji L, Huang Q, Vassylyev DG, Chen X, Ma JB.
Title
Structural insights into mechanisms of the small RNA methyltransferase HEN1.
Journal
Sequence
Reference
Authors
Peng L, Zhang F, Shang R, Wang X, Chen J, Chou JJ, Ma J, Wu L, Huang Y.
Title
Identification of substrates of the small RNA methyltransferase Hen1 in mouse spermatogonial stem cells and analysis of its methyl-transfer domain.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 2.1.1.386