Entry
Name
malonyl-S-ACP:biotin-protein carboxyltransferase;
malonyl-S-acyl-carrier protein:biotin-protein carboxyltransferase;
MadC/MadD;
MadC,D;
malonyl-[acyl-carrier protein]:biotinyl-[protein] carboxyltransferase
Class
Transferases;
Transferring one-carbon groups;
Carboxy- and carbamoyltransferases
BRITE hierarchy
Sysname
malonyl-[acyl-carrier protein]:biotinyl-[protein] carboxytransferase
Reaction(IUBMB)
a malonyl-[acyl-carrier protein] + a biotinyl-[protein] = an acetyl-[acyl-carrier protein] + a carboxybiotinyl-[protein] [RN:
R08936 ]
Reaction(KEGG)
Substrate
malonyl-[acyl-carrier protein] [CPD:
C01209 ];
biotinyl-[protein] [CPD:
C18046 ]
Product
acetyl-[acyl-carrier protein] [CPD:
C03939 ];
carboxybiotinyl-[protein] [CPD:
C18047 ]
Comment
Derived from the components MadC and MadD of the anaerobic bacterium Malonomonas rubra, this enzyme is a component of EC
7.2.4.4 , biotin-dependent malonate decarboxylase. The carboxy group is transferred from malonate to the cofactor of the biotin protein (MadF) with retention of configuration [2]. Similar to EC
4.1.1.87 , malonyl-S-ACP decarboxylase, which forms part of the biotin-independent malonate decarboxylase (EC
4.1.1.88 ), this enzyme also follows on from EC
2.3.1.187 , acetyl-S-ACP:malonate ACP transferase, and results in the regeneration of the acetyl-[acyl-carrier protein] [3].
History
EC 2.1.3.10 created 2008, modified 2018
Orthology
K20510 malonyl-S-ACP:biotin-protein carboxyltransferase subunit MadC
K20511 malonyl-S-ACP:biotin-protein carboxyltransferase subunit MadD
Genes
KPZ : KPNIH27_22440 KPNIH27_22445
KPB : FH42_14170 FH42_14175
KLW : DA718_03335(mdcE) DA718_03340
KGR : JJJ10_02970(mdcE) JJJ10_02975
KPAS : LUW96_03545 LUW96_03550(mdcE)
KLC : K7H21_02980(mdcE) K7H21_02985
KLM : BWI76_25005 BWI76_25010
CAMA : F384_01155 F384_01160
CTEL : GBC03_24695 GBC03_24700(mdcE)
SFO : Z042_24845 Z042_26175
RCP : RCAP_rcp00127(mdcE) RCAP_rcp00128(mdcB)
RAP : RHOA_4940(madC) RHOA_4941(madD)
ATI : AL072_31130 AL072_31135
AZZ : DEW08_20130(mdcE) DEW08_20135
DES : DSOUD_0385 DSOUD_0386
» show all
Taxonomy
Reference
Authors
Berg M, Hilbi H, Dimroth P
Title
Sequence of a gene cluster from Malonomonas rubra encoding components of the malonate decarboxylase Na+ pump and evidence for their function.
Journal
Sequence
Reference
2
Authors
Micklefield, J., Harris, K.J., Groger, S., Mocek, U., Hilbi, H., Dimroth, P. and Floss, H.G.
Title
Stereochemical course of malonate decarboxylase in Malonomonas rubra has biotin decarboxylation with retention.
Journal
J Am Chem Soc 117:1153-1154 (1995)
Reference
Authors
Dimroth P, Hilbi H
Title
Enzymic and genetic basis for bacterial growth on malonate.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 2.1.3.10
ExPASy - ENZYME nomenclature database: 2.1.3.10