KEGG   ENZYME: 2.3.1.162
Entry
EC 2.3.1.162                Enzyme                                 
Name
taxadien-5alpha-ol O-acetyltransferase;
acetyl coenzyme A:taxa-4(20),11(12)-dien-5alpha-ol O-acetyl transferase
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
acetyl-CoA:taxa-4(20),11-dien-5alpha-ol O-acetyltransferase
Reaction(IUBMB)
acetyl-CoA + taxa-4(20),11-dien-5alpha-ol = CoA + taxa-4(20),11-dien-5alpha-yl acetate [RN:R06307]
Reaction(KEGG)
R06307
Substrate
acetyl-CoA [CPD:C00024];
taxa-4(20),11-dien-5alpha-ol [CPD:C11895]
Product
CoA [CPD:C00010];
taxa-4(20),11-dien-5alpha-yl acetate
Comment
This is the third enzyme in the biosynthesis of the diterpenoid antineoplastic drug taxol (paclitaxel), which is widely used in the treatment of carcinomas, sarcomas and melanomas.
History
EC 2.3.1.162 created 2002
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K12923  taxadien-5alpha-ol O-acetyltransferase
Reference
1  [PMID:10190984]
  Authors
Walker K, Ketchum RE, Hezari M, Gatfield D, Goleniowski M, Barthol A, Croteau R.
  Title
Partial purification and characterization of acetyl coenzyme A: taxa-4(20),11(12)-dien-5alpha-ol O-acetyl transferase that catalyzes the first acylation step of taxol biosynthesis.
  Journal
Arch Biochem Biophys 364:273-9 (1999)
DOI:10.1006/abbi.1999.1125
Reference
2  [PMID:10666320]
  Authors
Walker K, Schoendorf A, Croteau R
  Title
Molecular cloning of a taxa-4(20),11(12)-dien-5alpha-ol-O-acetyl transferase cDNA from Taxus and functional expression in Escherichia coli.
  Journal
Arch Biochem Biophys 374:371-80 (2000)
DOI:10.1006/abbi.1999.1609
  Sequence
[ag:AAF34254]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.162
IUBMB Enzyme Nomenclature: 2.3.1.162
ExPASy - ENZYME nomenclature database: 2.3.1.162
BRENDA, the Enzyme Database: 2.3.1.162
CAS: 229032-29-5

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Pathway (4)   
   KEGG PATHWAY (4)   
Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (3)   
   KEGG REACTION (1)   
   KEGG RCLASS (2)   
Gene (2)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (1)   
Protein sequence (42)   
   UniProt (40)   
   SWISS-PROT (2)   
DNA sequence (9)   
   GenBank (9)   
Protein domain (2)   
   InterPro (2)   
All databases (66)   

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