KEGG   ENZYME: 2.3.1.244
Entry
EC 2.3.1.244                Enzyme                                 
Name
2-methylbutanoate polyketide synthase;
LovF
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
acyl-CoA:malonyl-CoA C-acyltransferase (2-methylbutanoate-forming)
Reaction(IUBMB)
2 malonyl-CoA + [2-methylbutanoate polyketide synthase] + 2 NADPH + 3 H+ + S-adenosyl-L-methionine = (S)-2-methylbutanoyl-[2-methylbutanoate polyketide synthase] + 2 CoA + 2 CO2 + 2 NADP+ + S-adenosyl-L-homocysteine + H2O [RN:R11445]
Reaction(KEGG)
R11445
Substrate
malonyl-CoA [CPD:C00083];
[2-methylbutanoate polyketide synthase];
NADPH [CPD:C00005];
H+ [CPD:C00080];
S-adenosyl-L-methionine [CPD:C00019]
Product
(S)-2-methylbutanoyl-[2-methylbutanoate polyketide synthase];
CoA [CPD:C00010];
CO2 [CPD:C00011];
NADP+ [CPD:C00006];
S-adenosyl-L-homocysteine [CPD:C00021];
H2O [CPD:C00001]
Comment
This polyketide synthase enzyme forms the (S)-2-methylbutanoate side chain during lovastatin biosynthesis by the filamentous fungus Aspergillus terreus. The overall reaction comprises a single condensation reaction followed by alpha-methylation, beta-ketoreduction, dehydration, and alpha,beta-enoyl reduction.
History
EC 2.3.1.244 created 2015, modified 2016
Pathway
ec00997  Biosynthesis of various other secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K20984  2-methylbutanoate polyketide synthase
Reference
1  [PMID:10334994]
  Authors
Kennedy J, Auclair K, Kendrew SG, Park C, Vederas JC, Hutchinson CR
  Title
Modulation of polyketide synthase activity by accessory proteins during lovastatin biosynthesis.
  Journal
Science 284:1368-72 (1999)
DOI:10.1126/science.284.5418.1368
  Sequence
[ag:AAD34559]
Reference
2  [PMID:21069965]
  Authors
Meehan MJ, Xie X, Zhao X, Xu W, Tang Y, Dorrestein PC
  Title
FT-ICR-MS characterization of intermediates in the biosynthesis of the alpha-methylbutyrate side chain of lovastatin by the 277 kDa polyketide synthase LovF.
  Journal
Biochemistry 50:287-99 (2011)
DOI:10.1021/bi1014776
  Sequence
[ag:AAD34559]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.244
IUBMB Enzyme Nomenclature: 2.3.1.244
ExPASy - ENZYME nomenclature database: 2.3.1.244
BRENDA, the Enzyme Database: 2.3.1.244

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Pathway (7)   
   KEGG PATHWAY (6)   
   KEGG MODULE (1)   
Chemical substance (11)   
   KEGG COMPOUND (11)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (2)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (1)   
Protein sequence (8)   
   UniProt (4)   
   SWISS-PROT (4)   
Protein domain (27)   
   InterPro (27)   
All databases (57)   

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