KEGG ENZYME: 2.3.1.246

ENZYME: 2.3.1.246

Entry

EC 2.3.1.246                Enzyme

Name

3,5-dihydroxyphenylacetyl-CoA synthase;
DpgA

Class

Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups

Sysname

malonyl-CoA:malonyl-CoA malonyltransferase (3,5-dihydroxyphenylacetyl-CoA-forming)

Reaction(IUBMB)

4 malonyl-CoA = (3,5-dihydroxyphenylacetyl)-CoA + 3 CoA + 4 CO2 + H2O [RN:R06625]

Reaction(KEGG)

R06625

Substrate

malonyl-CoA [CPD:C00083]

Product

3,5-dihydroxyphenylacetyl-CoA [CPD:C12324];
CoA [CPD:C00010];
CO2 [CPD:C00011];
H2O [CPD:C00001]

Comment

The enzyme, characterized from the bacterium Amycolatopsis mediterranei, is involved in biosynthesis of the nonproteinogenic amino acid (S)-3,5-dihydroxyphenylglycine, a component of the vancomycin-type antibiotic balhimycin.

History

EC 2.3.1.246 created 2015

Pathway

ec01055

Biosynthesis of vancomycin group antibiotics

ec01110

Biosynthesis of secondary metabolites

Orthology

K16424

3,5-dihydroxyphenylacetyl-CoA synthase

Genes

SMAF:

D781_2331

SERM:

CLM71_12100

RBAD:

H2866_19105

XAL:

XALC_1059

XTN:	FD63_09790

XPR:	MUG10_21500

PAND:

DRB87_09790

MNR:	ACZ75_13535

BSTO:

C0V70_02630

NBR:	O3I_032820

NTP:	CRH09_30870 CRH09_31745

NAD:	NCTC11293_04865

NIE:	KV110_15715

NGP:	LTT66_10445

SRC:	M271_00240 M271_09145

SMAL:

SMALA_7737

SSOI:

I1A49_42475

SBH:

SBI_03177

SVE:

SVEN_0493

SCW:	TU94_04905 TU94_32245

STRC:

AA958_31865

SLE:	sle_03950(sle_03950) sle_58620(sle_58620)

STSI:

A4E84_04190

SKY:	D0C37_02210

SALW:

CP975_31980

SPHW:

NFX46_19255

SSIA:

A7J05_04090

SFK:	KY5_6273 KY5_7440

SKA:	CP970_02995 CP970_09670

SGZ:	C0216_32530

SGAL:

CP966_31750

SAQU:

EJC51_23015

STSU:

B7R87_00855

SVD:	CP969_02990

SROI:

IAG44_40635

KAU:	B6264_25030

STRH:

GXP74_22695

KFL:

Kfla_4132

NDA:

Ndas_1740

ACTW:

F7P10_10120

AGRA:

AGRA3207_002330

AMAZ:

LUW76_44605

NOA:	BKM31_54175

NCX:	Nocox_13295(noc8)

NGN:	LCN96_07940

FRA:	Francci3_2458

SACC:

EYD13_07740

AOI:	AORI_1502(bcsA) AORI_5330(bcsA)

AJA:	AJAP_32005

AMYB:

BKN51_41555

AAB:	A4R43_07900

AMYY:

YIM_22225

AORI:

SD37_33610

AROO:

NQK81_15375

KUT:	JJ691_30260 JJ691_70290

KBU:	Q4V64_04805

MTEM:

GCE86_08035

MSAG:

GCM10017556_56550

MFEU:

H1D33_24995(dpgA)

ACTL:

L3i22_092840(pks18)

AOU:	ACTOB_006418 ACTOB_006457

PFLA:

Pflav_008040

PSUU:

Psuf_070250

PRY:	Prubr_65020(pks18)

MPRO:

BJP34_31700

PUO:	RZN69_11825

» show all

Reference

1 [PMID:11495926]

Authors

Pfeifer V, Nicholson GJ, Ries J, Recktenwald J, Schefer AB, Shawky RM, Schroder J, Wohlleben W, Pelzer S

Title

A polyketide synthase in glycopeptide biosynthesis: the biosynthesis of the non-proteinogenic amino acid (S)-3,5-dihydroxyphenylglycine.

Journal

J Biol Chem 276:38370-7 (2001)
DOI:10.1074/jbc.M106580200

Sequence

[ag:CAC48378]

Reference

2 [PMID:11752437]

Authors

Chen H, Tseng CC, Hubbard BK, Walsh CT

Title

Glycopeptide antibiotic biosynthesis: enzymatic assembly of the dedicated amino acid monomer (S)-3,5-dihydroxyphenylglycine.

Journal

Proc Natl Acad Sci U S A 98:14901-6 (2001)
DOI:10.1073/pnas.221582098

Reference

3 [PMID:14744141]

Authors

Tseng CC, McLoughlin SM, Kelleher NL, Walsh CT

Title

Role of the active site cysteine of DpgA, a bacterial type III polyketide synthase.

Journal

Biochemistry 43:970-80 (2004)
DOI:10.1021/bi035714b

Reference

4 [PMID:22492619]

Authors

Wu HC, Li YS, Liu YC, Lyu SY, Wu CJ, Li TL

Title

Chain elongation and cyclization in type III PKS DpgA.

Journal

Chembiochem 13:862-71 (2012)
DOI:10.1002/cbic.201200051

Other DBs

ExplorEnz - The Enzyme Database:

2.3.1.246

IUBMB Enzyme Nomenclature:

2.3.1.246

ExPASy - ENZYME nomenclature database:

2.3.1.246

BRENDA, the Enzyme Database:

2.3.1.246

DBGET integrated database retrieval system