KEGG ENZYME: 2.3.1.282

ENZYME: 2.3.1.282

Entry

EC 2.3.1.282                Enzyme

Name

phenolphthiocerol/phthiocerol/phthiodiolone dimycocerosyl transferase;
papA5 (gene name)

Class

Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups

Sysname

mycocerosyl-[mycocerosic acid synthase]:phenolphthiocerol/phthiocerol/phthiodiolone dimycocerosyl transferase

Reaction(IUBMB)

(1) 2 a mycocerosyl-[mycocerosic acid synthase] + a phthiocerol = a dimycocerosyl phthiocerol + 2 holo-[mycocerosic acid synthase];
(2) 2 a mycocerosyl-[mycocerosic acid synthase] + a phthiodiolone = a dimycocerosyl phthiodiolone + 2 holo-[mycocerosic acid synthase];
(3) 2 a mycocerosyl-[mycocerosic acid synthase] + a phenolphthiocerol = a dimycocerosyl phenolphthiocerol + 2 holo-[mycocerosic acid synthase]

Substrate

a mycocerosyl-[mycocerosic acid synthase];
phthiocerol;
phthiodiolone;
phenolphthiocerol

Product

dimycocerosyl phthiocerol;
holo-[mycocerosic acid synthase];
dimycocerosyl phthiodiolone;
dimycocerosyl phenolphthiocerol

Comment

The enzyme, present in certain pathogenic species of mycobacteria, catalyses the transfer of mycocerosic acids to the two hydroxyl groups at the common lipid core of phthiocerol, phthiodiolone, and phenolphthiocerol, forming dimycocerosate esters. The fatty acid precursors of mycocerosic acids are activated by EC 6.2.1.49, long-chain fatty acid adenylyltransferase FadD28, which loads them onto EC 2.3.1.111, mycocerosate synthase. That enzyme extends the precursors to form mycocerosic acids that remain attached until transferred by EC 2.3.1.282.

History

EC 2.3.1.282 created 2019

Orthology

K23414

phenolphthiocerol/phthiocerol/phthiodiolone dimycocerosyl transferase

Genes

MTU:	Rv2939(papA5)

MTV:

RVBD_2939

MTC:

MT3009

MRA:	MRA_2965(papA5)

MTF:	TBFG_12953

MTB:	TBMG_01033

MTK:	TBSG_01041

MTZ:	TBXG_001021

MTG:	MRGA327_18065

MTE:	CCDC5079_2699

MTUR:

CFBS_3097(papA5)

MTL:	CCDC5180_2663

MTO:	MTCTRI2_2996(papA5)

MTD:	UDA_2939(papA5)

MTN:	ERDMAN_3221(papA5)

MTJ:	J112_15730

MTUB:

MT7199_2973

MTUC:

J113_20430

MTUE:

J114_15695

MTX:	M943_15155

MTUL:

TBHG_02869

MTUT:

HKBT1_3085(papA5)

MTUU:

HKBT2_3090(papA5)

MTQ:	HKBS1_3092(papA5)

MBO:	BQ2027_MB2964(papA5)

MBB:	BCG_2961(papA5)

MBT:	JTY_2956(papA5)

MBM:	BCGMEX_2956(papA5)

MBK:	K60_030440

MBX:

BCGT_2777

MAF:	MAF_29440(papA5)

MMIC:

RN08_3238

MCE:	MCAN_29611(papA5)

MCQ:	BN44_60416(papA)

MCV:	BN43_40647(papA)

MCX:	BN42_40937(papA)

MCZ:	BN45_51351(papA)

MORY:

MO_003071

MLE:

ML2349

MLB:

MLBr02349

MSA:	Mycsm_05871

MUL:	MUL_2011(papA5)

MMI:	MMAR_1768(papA5)

MMAE:

MMARE11_16880(papA5)

MLI:	MULP_01924(papA5)

MPSE:

MPSD_40660(papA5)

MSHO:

MSHO_54720(papA5)

MMC:

Mmcs_2828

MKM:

Mkms_2872

MJL:

Mjls_2857

MKN:	MKAN_24000

MHAD:

B586_03535

MDX:	BTO20_18105

MSHG:

MSG_01623(papA5)

MFJ:	MFLOJ_16830(papA5)

MSTO:

MSTO_18420(papA5)

MSIM:

MSIM_07810(papA5)

MLW:	MJO58_08790

MPAG:

C0J29_10290

MNM:	MNVM_38420(papA5)

MCOO:

MCOO_18260(papA5)

MBAI:

MB901379_01585(papA5)

MGAU:

MGALJ_49450(papA5)

MLJ:	MLAC_28750(papA5)

MSHJ:

MSHI_07280(papA5)

MMAM:

K3U93_07805

MSPG:

F6B93_07125

MOT:	LTS72_15970

MLM:

MLPF_2691

MPAA:

MKK62_06600

MMEH:

M5I08_15240 M5I08_15295

MWU:	PT015_10800

MVA:

Mvan_3119

MGI:

Mflv_3386

MSP:	Mspyr1_27170

MCB:	Mycch_2769

MVQ:	MYVA_2956(papA5)

MDU:	MDUV_13070(papA5)

MAUU:

NCTC10437_03014(papA5)

MPSC:

MPSYJ_36520(papA5)

MPOF:

MPOR_33480(papA5)

MSEI:

MSEDJ_33280(papA5)

MMON:

EWR22_14995

MRF:	MJO55_13995

MKR:	MKOR_16460(papA5) MKOR_25080

MPAK:

MIU77_12280

MMV:	MYCMA_02745

MABB:

MASS_4932

MABL:

MMASJCM_2046 MMASJCM_3096 MMASJCM_4925

MCHE:

BB28_20865 BB28_24715

MIZ:	BAB75_07595 BAB75_16630 BAB75_27425

MSTE:

MSTE_04219

MSAO:

MYCSP_14000 MYCSP_22545

MSAL:

DSM43276_03871(papA5_2) DSM43276_04662(papA5_3)

MJD:	JDM601_2209(papA5)

MTER:

4434518_02132(papA5)

MMIN:

MMIN_38070(papA5)

MHIB:

MHIB_16580(papA5)

MHER:

K3U94_12435

MVM:	MJO54_12380

NFA:

NFA_14610

NFR:	ERS450000_02658(papA5)

NCY:	NOCYR_1519 NOCYR_2391 NOCYR_5047

NBR:	O3I_006920 O3I_008400 O3I_008740 O3I_009815 O3I_022155 O3I_033785

NSL:	BOX37_07180

NSR:	NS506_02382 NS506_02688

NTP:	CRH09_08050 CRH09_14155 CRH09_20665 CRH09_37215

NOZ:	DMB37_17945 DMB37_27790 DMB37_30560

NAH:	F5544_08085 F5544_21435 F5544_30935 F5544_30945 F5544_31775 F5544_41640 F5544_44790

NAD:	NCTC11293_00161(papA5_1) NCTC11293_02450(papA5_2)

NWL:	NWFMUON74_66390

NIE:	KV110_07475 KV110_10345

NHU:	H0264_22540

NGP:	LTT66_03835

NSPU:

IFM12276_47310(papA5)

RFA:	A3L23_04949(papA5)

GOI:	LK459_21355

TPR:	Tpau_1036 Tpau_1039 Tpau_1040 Tpau_1646

TSM:	ASU32_17860 ASU32_17865 ASU32_17880

TPUL:

TPB0596_38030(papA5_2)

SRT:	Srot_1147 Srot_1825 Srot_2445 Srot_2530 Srot_2531 Srot_2532 Srot_2533 Srot_2688

SALB:

XNR_5892

SHY:

SHJG_1266

SHO:	SHJGH_1101

SAMB:

SAM23877_0429

SCX:	AS200_43245

SRW:	TUE45_pSRc_0201(papA5)

STRF:

ASR50_01520

SKY:	D0C37_31720

SLS:	SLINC_8386

SPLU:

LK06_031380

SSIA:

A7J05_33445

SPUN:

BFF78_27180

SLAU:

SLA_0410

SALJ:

SMD11_0468

SRJ:

SRO_0455

SGD:	ELQ87_00185

SAST:

CD934_28920

SVR:	CP971_08170

SGF:	HEP81_00439

SFUG:

CNQ36_01475

SSPB:

CP982_05710 CP982_35270

SACT:

DMT42_00495

SRUG:

F0345_01210

SVIO:

HWN34_29800

STEE:

F3L20_18770

SCY:	SCATT_p16520

FSY:	FsymDg_2299

AOI:

AORI_5400

AORI:

SD37_10955

AMI:

Amir_3883

KAL:

KALB_5458

ACTN:

L083_5715

» show all

Reference

1 [PMID:15070765]

Authors

Onwueme KC, Ferreras JA, Buglino J, Lima CD, Quadri LE

Title

Mycobacterial polyketide-associated proteins are acyltransferases: proof of principle with Mycobacterium tuberculosis PapA5.

Journal

Proc Natl Acad Sci U S A 101:4608-13 (2004)
DOI:10.1073/pnas.0306928101

Sequence

[mtu:Rv2939]

Reference

2 [PMID:15123643]

Authors

Buglino J, Onwueme KC, Ferreras JA, Quadri LE, Lima CD

Title

Crystal structure of PapA5, a phthiocerol dimycocerosyl transferase from Mycobacterium tuberculosis.

Journal

J Biol Chem 279:30634-42 (2004)
DOI:10.1074/jbc.M404011200

Sequence

[mtu:Rv2939]

Reference

3 [PMID:22361940]

Authors

Chavadi SS, Onwueme KC, Edupuganti UR, Jerome J, Chatterjee D, Soll CE, Quadri LE

Title

The mycobacterial acyltransferase PapA5 is required for biosynthesis of cell wall-associated phenolic glycolipids.

Journal

Microbiology 158:1379-87 (2012)
DOI:10.1099/mic.0.057869-0

Reference

4 [PMID:26271001]

Authors

Touchette MH, Bommineni GR, Delle Bovi RJ, Gadbery JE, Nicora CD, Shukla AK, Kyle JE, Metz TO, Martin DW, Sampson NS, Miller WT, Tonge PJ, Seeliger JC

Title

Diacyltransferase Activity and Chain Length Specificity of Mycobacterium tuberculosis PapA5 in the Synthesis of Alkyl beta-Diol Lipids.

Journal

Biochemistry 54:5457-68 (2015)
DOI:10.1021/acs.biochem.5b00455

Sequence

[mtu:Rv2939]

Other DBs

ExplorEnz - The Enzyme Database:

2.3.1.282

IUBMB Enzyme Nomenclature:

2.3.1.282

ExPASy - ENZYME nomenclature database:

2.3.1.282

BRENDA, the Enzyme Database:

2.3.1.282

All links

Gene (197)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (196)   
Protein sequence (1632)   
   UniProt (1621)   
   SWISS-PROT (11)   
Protein domain (2)   
   InterPro (2)   
All databases (1831)   

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