KEGG   ENZYME: 2.3.1.293
Entry
EC 2.3.1.293                Enzyme                                 
Name
meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase I;
kasA (gene name);
beta-ketoacyl-acyl carrier protein synthase KasA
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
ultra-long-chain mono-unsaturated fattyl acyl-[acyl-carrier protein]:malonyl-[acyl-carrier protein] C-acyltransferase (decarboxylating)
Reaction(IUBMB)
an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + a holo-[acyl-carrier protein] [RN:R13266]
Reaction(KEGG)
R13266;
(other) R13258 R13262
Substrate
ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] [CPD:C22865];
malonyl-[acyl-carrier protein] [CPD:C01209]
Product
ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] [CPD:C22866];
CO2 [CPD:C00011];
holo-[acyl-carrier protein] [CPD:C00229]
Comment
The enzyme is part of the fatty acid synthase (FAS) II system of mycobacteria, which extends modified products of the FAS I system, eventually forming meromycolic acids that are incorporated into mycolic acids. Meromycolic acids consist of a long chain, typically 50-60 carbons, which is functionalized by different groups.Two 3-oxoacyl-(acyl carrier protein) synthases function within the FAS II system, encoded by the kasA and kasB genes. The two enzymes share some sequence identity but function independently on separate sets of substrates. KasA differs from KasB [EC 2.3.1.294, meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase II], by preferring shorter (C-22 to C-36) and more saturated (only one double bond) substrates.
History
EC 2.3.1.293 created 2019
Orthology
K11609  meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase I
Genes
MTU: Rv2245(kasA)
MTV: RVBD_2245
MTC: MT2305(fabF-1)
MRA: MRA_2265(kasA)
MTF: TBFG_12274
MTB: TBMG_01735
MTK: TBSG_01745
MTZ: TBXG_001717
MTG: MRGA327_13860
MTI: MRGA423_13980
MTE: CCDC5079_2082
MTUR: CFBS_2378
MTL: CCDC5180_2054
MTO: MTCTRI2_2281(kasA)
MTD: UDA_2245(kasA)
MTN: ERDMAN_2470(kasA)
MTJ: J112_12055
MTUB: MT7199_2277
MTUE: J114_12035
MTX: M943_11630
MTUH: I917_15810
MTUL: TBHG_02197
MTUT: HKBT1_2369
MTUU: HKBT2_2371
MTQ: HKBS1_2375
MBO: BQ2027_MB2269(kasA)
MBB: BCG_2262(kasA)
MBT: JTY_2256(kasA)
MBM: BCGMEX_2250(kasA)
MBK: K60_023300
MBX: BCGT_2068
MAF: MAF_22560(kasA)
MMIC: RN08_2493
MCE: MCAN_22671(kasA)
MCQ: BN44_50188(kasA)
MCV: BN43_31488(kasA)
MCX: BN42_40169(kasA)
MCZ: BN45_50577(kasA)
MORY: MO_002354(kasA)
MLE: ML1655(kasA)
MLB: MLBr01655(kasA)
MPA: MAP_1998(kasA) MAP_3485(kasB)
MAO: MAP4_0292 MAP4_1827
MAVI: RC58_09130
MAVU: RE97_09135
MAV: MAV_2192 MAV_4343
MIT: OCO_21790 OCO_42090
MIA: OCU_21970 OCU_42010
MID: MIP_03078 MIP_06344
MYO: OEM_19680 OEM_42440
MCHI: AN480_11275 AN480_22070
MIR: OCQ_20900 OCQ_43360
MMAL: CKJ54_09725 CKJ54_20115
MLP: MLM_1921
MMAN: MMAN_18020(kasA) MMAN_30190(kasB_2)
MSA: Mycsm_03980
MUL: MUL_1304(kasA)
MMI: MMAR_3338(kasA)
MMAE: MMARE11_32390(kasA)
MLI: MULP_03599(kasA)
MPSE: MPSD_34150(kasA)
MSHO: MSHO_47500(kasA)
MMC: Mmcs_3366
MKM: Mkms_3428
MJL: Mjls_3377
MMM: W7S_10245 W7S_21055
MKN: MKAN_03345
MYV: G155_18575
MYE: AB431_19260
MHAD: B586_09895
MDX: BTO20_13090
MSHG: MSG_01053(kasB_1) MSG_03170(kasA)
MFJ: MFLOJ_00460(kasA) MFLOJ_23320(kasB_2)
MSTO: MSTO_01120(kasA) MSTO_27280(kasB_2)
MSIM: MSIM_14500(kasB_1) MSIM_45160(kasA)
MSAK: MSAS_12200(kasA)
MKU: I2456_16055
MLW: MJO58_05580(kasB) MJO58_16830(kasA)
MXE: MYXE_20730(kasA)
MNV: MNVI_45150(kasA)
MPAG: C0J29_18640
MNM: MNVM_02370(kasA) MNVM_40280
MGOR: H0P51_17360
MCOO: MCOO_12820
MBAI: MB901379_03010(kasA_1)
MSEO: MSEO_19570(kasA)
MHEK: JMUB5695_02082(kasA)
MGAU: MGALJ_21310
MLJ: MLAC_22590(kasA)
MBRD: MBRA_35620(kasA)
MSHJ: MSHI_10170(kasA)
MMAM: K3U93_14875
MSPG: F6B93_13965
MOT: LTS72_07820
MLM: MLPF_0930(kasA)
MPAA: MKK62_10060(kasA)
MMEH: M5I08_12045(kasA)
MKY: IWGMT90018_24130(kasA)
MADI: A7U43_13135
MWU: PT015_07475(kasA)
MSM: MSMEG_4327
MSG: MSMEI_4227(kasA)
MSB: LJ00_21445
MSN: LI99_21450
MSH: LI98_21455
MVA: Mvan_3756 Mvan_4950
MGI: Mflv_1799 Mflv_2777
MSP: Mspyr1_11830 Mspyr1_22170
MCB: Mycch_3278 Mycch_5138
MNE: D174_18305
MYN: MyAD_17950
MFT: XA26_37930
MPHL: MPHLCCUG_03392(kasA)
MVQ: MYVA_3578 MYVA_4769 MYVA_5709
MLL: B1R94_10590
MRH: MycrhN_4435
MTHN: 4412656_01786(kasA_1)
MHAS: MHAS_01449(kasA)
MDU: MDUV_08140 MDUV_44660
MCHT: MCHIJ_33640
MDR: MDOR_24810
MAUU: NCTC10437_03513(kasA) NCTC10437_04668(kasB_2)
MMAG: MMAD_09900(kasB_1) MMAD_35130 MMAD_44180(kasB_2)
MMOR: MMOR_29440
MFX: MFAL_13630
MAIC: MAIC_34210
MIJ: MINS_32340
MALV: MALV_02280
MTY: MTOK_56270
MPSC: MPSYJ_10760 MPSYJ_30190
MARZ: MARA_52190 MARA_61350
MGAD: MGAD_02950
MHEV: MHEL_60250
MSAR: MSAR_35170
MANY: MANY_19070
MAUB: MAUB_50720
MPOF: MPOR_39290 MPOR_50690(kasB)
MPHU: MPHO_18690
MMUC: C1S78_009095
MMAT: MMAGJ_73560
MBOK: MBOE_36000
MFG: K6L26_13130
MSEI: MSEDJ_47240 MSEDJ_57810
MFLV: NCTC10271_01899(kasA)
MCEE: MCEL_03190
MBRM: L2Z93_001323(kasA)
MMON: EWR22_17420
MGO: AFA91_18470
MHOL: K3U96_10650
MSEN: K3U95_15995
MPAE: K0O64_18625
MRF: MJO55_16900(kasA) MJO55_26740
MDF: K0O62_18260
MCRO: MI149_19450(kasA)
MGRO: FZ046_00350 FZ046_10765 FZ046_22645
MKR: MKOR_04710(kasA)
MPAK: MIU77_06600(kasA)
MAB: MAB_1877c MAB_4608
MMV: MYCMA_0969 MYCMA_2544
MABB: MASS_1864 MASS_4635
MABL: MMASJCM_0440 MMASJCM_1795 MMASJCM_4664
MCHE: BB28_09685 BB28_23520
MIZ: BAB75_10110 BAB75_25995
MSTE: MSTE_01795 MSTE_04780
MSAO: MYCSP_08125 MYCSP_21495
MSAL: DSM43276_01633(kasA_1) DSM43276_04441(kasA_2)
MJD: JDM601_1650(kasA)
MTER: 4434518_01563(kasA) 4434518_01976(kasB_2)
MMIN: MMIN_32500(kasA)
MHIB: MHIB_10840(kasA) MHIB_26020
MHER: K3U94_09080 K3U94_17975
MVM: MJO54_09170(kasA) MJO54_18130(kasB)
ASD: AS9A_1223(fabF)
NFA: NFA_16170(kasA)
NFR: ERS450000_02543(kasA_1)
NCY: NOCYR_1672(fabF) NOCYR_5164(kasA)
NBR: O3I_001035 O3I_001530 O3I_010905 O3I_039885
NNO: NONO_c06540(kasA1) NONO_c13390(kasA2) NONO_c20820(kasA3) NONO_c39590(kasA4) NONO_c40520(kasA5)
NSL: BOX37_05410 BOX37_07850 BOX37_30445
NSR: NS506_02999 NS506_04587 NS506_06033
NTP: CRH09_07575 CRH09_11630 CRH09_18150
NOZ: DMB37_18610 DMB37_22095 DMB37_22610 DMB37_31465
NOD: FOH10_06500 FOH10_13310 FOH10_19595 FOH10_24550
NAH: F5544_08130 F5544_14420 F5544_42295
NAD: NCTC11293_00291(kasA_1) NCTC11293_02031(kasA_2) NCTC11293_02578(kasA_4)
NWL: NWFMUON74_33840 NWFMUON74_34890 NWFMUON74_51900 NWFMUON74_58450
NIE: KV110_01045 KV110_10935 KV110_36485 KV110_38945
NTC: KHQ06_15840 KHQ06_23050 KHQ06_31165 KHQ06_37615
NHU: H0264_15065 H0264_21500 H0264_22775 H0264_30405 H0264_36750
NYA: LTV02_03220 LTV02_10585 LTV02_18070 LTV02_36510
NGP: LTT66_00620 LTT66_03330 LTT66_09725 LTT66_13895
NSPU: IFM12276_39730 IFM12276_46210 IFM12276_59480 IFM12276_66290
NYU: D7D52_10910 D7D52_24465 D7D52_35270 D7D52_35590
RHA: RHA1_ro01201(fabF)
RER: RER_36740(fabF)
REY: O5Y_16895
REB: XU06_17105
RQI: C1M55_18105
ROP: ROP_09240(fabF) ROP_12330(fabF)
ROA: Pd630_LPD05302 Pd630_LPD05661
RPY: Y013_06230
RBY: CEJ39_17015
RHB: NY08_584
RAV: AAT18_15865
RFA: A3L23_03833(kasA)
RHS: A3Q41_04409(kasA)
RHW: BFN03_10195
RRZ: CS378_05515
RHU: A3Q40_01110(kasA)
RHQ: IM25_20245
RHOD: AOT96_28425 AOT96_30850
RRT: 4535765_03064(fabF_2)
RCR: NCTC10994_04197(fabF_3)
RTM: G4H71_01320
RKO: JWS14_06230
RGO: KYT97_25490
ROZ: CBI38_11880
RPSK: JWS13_38335 JWS13_40200
RGOR: NMQ04_13090
RHOP: D8W71_04420 D8W71_19330
REQ: REQ_28970(kasA)
PDEF: P9209_14735
WHR: OG579_04820
GBR: Gbro_3123
GPO: GPOL_c28870(kasA)
GOR: KTR9_3116
GOQ: ACH46_13020
GTA: BCM27_16010
GIT: C6V83_13400
GRU: GCWB2_16225(kasA)
GOM: D7316_00150(kasA)
GAV: C5O27_11985
GOD: GKZ92_14975
GAM: GII34_14920
GPD: GII33_14045
GJI: H1R19_15115
GOI: LK459_18890
GHN: MVF96_15495
GAMI: IHQ52_18855
GMG: NWF22_00635
GSI: P5P27_12410
SPIN: KV203_06400
TPR: Tpau_2468 Tpau_2726
TSM: ASU32_13575 ASU32_15195
TPUL: TPB0596_17510 TPB0596_21010
TSD: MTP03_27750 MTP03_31280
SRT: Srot_0300 Srot_2738
TOY: FO059_12230
 » show all
Reference
1  [PMID:11600501]
  Authors
Schaeffer ML, Agnihotri G, Volker C, Kallender H, Brennan PJ, Lonsdale JT
  Title
Purification and biochemical characterization of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthases KasA and KasB.
  Journal
J Biol Chem 276:47029-37 (2001)
DOI:10.1074/jbc.M108903200
  Sequence
[mtu:Rv2245]
Reference
2  [PMID:16267284]
  Authors
Bhatt A, Kremer L, Dai AZ, Sacchettini JC, Jacobs WR Jr
  Title
Conditional depletion of KasA, a key enzyme of mycolic acid biosynthesis, leads to mycobacterial cell lysis.
  Journal
J Bacteriol 187:7596-606 (2005)
DOI:10.1128/JB.187.22.7596-7606.2005
Reference
3  [PMID:19604480]
  Authors
Luckner SR, Machutta CA, Tonge PJ, Kisker C
  Title
Crystal structures of Mycobacterium tuberculosis KasA show mode of action within cell wall biosynthesis and its inhibition by thiolactomycin.
  Journal
Structure 17:1004-13 (2009)
DOI:10.1016/j.str.2009.04.012
  Sequence
[mtu:Rv2245]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.293
IUBMB Enzyme Nomenclature: 2.3.1.293
ExPASy - ENZYME nomenclature database: 2.3.1.293
BRENDA, the Enzyme Database: 2.3.1.293

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Chemical substance (9)   
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   KEGG GENES (356)   
   KEGG MGENES (2)   
Protein sequence (10)   
   UniProt (5)   
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