KEGG   ENZYME: 2.3.1.294
Entry
EC 2.3.1.294                Enzyme                                 
Name
meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase II;
kasB (gene name);
beta-ketoacyl-acyl carrier protein synthase KasB
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
ultra-long-chain di-unsaturated fattyl acyl-[acyl-carrier protein]:malonyl-[acyl-carrier protein] C-acyltransferase (decarboxylating)
Reaction(IUBMB)
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + a holo-[acyl-carrier protein]
Substrate
ultra-long-chain di-unsaturated acyl-[acyl-carrier protein];
malonyl-[acyl-carrier protein] [CPD:C01209]
Product
ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein];
CO2 [CPD:C00011];
holo-[acyl-carrier protein] [CPD:C00229]
Comment
The enzyme is part of the fatty acid synthase (FAS) II system of mycobacteria, which extends modified products of the FAS I system, eventually forming meromycolic acids that are incorporated into mycolic acids. Meromycolic acids consist of a long chain, typically 50-60 carbons, which is functionalized by different groups.Two 3-oxoacyl-(acyl carrier protein) synthases function within the FAS II system, encoded by the kasA and kasB genes. The two enzymes share some sequence identity but function independently on separate sets of substrates. KasB differs from KasA (EC 2.3.1.293, meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase I), by preferring longer substrates (closer to the upper limit), which also contain two double bonds.
History
EC 2.3.1.294 created 2019
Orthology
K27070  meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase II
Genes
MTU: Rv2246(kasB)
MTV: RVBD_2246
MTC: MT2306(fabF-2)
MRA: MRA_2266(kasB)
MTF: TBFG_12275
MTB: TBMG_01734
MTK: TBSG_01744
MTZ: TBXG_001716
MTI: MRGA423_13985
MTE: CCDC5079_2083
MTUR: CFBS_2379(kasB)
MTL: CCDC5180_2055
MTO: MTCTRI2_2282(kasB)
MTD: UDA_2246(kasB)
MTN: ERDMAN_2471(kasB)
MTJ: J112_12060
MTUB: MT7199_2278
MTUE: J114_12040
MTX: M943_11635
MTUL: TBHG_02198
MTUT: HKBT1_2370(kasB)
MTUU: HKBT2_2372(kasB)
MTQ: HKBS1_2376(kasB)
MBO: BQ2027_MB2270(kasB)
MBB: BCG_2263(kasB)
MBT: JTY_2257(kasB)
MBM: BCGMEX_2251(kasB)
MBK: K60_023310
MBX: BCGT_2069
MAF: MAF_22570(kasB)
MMIC: RN08_2494
MCE: MCAN_22681(kasB)
MCQ: BN44_50189(kasB)
MCV: BN43_31489(kasB)
MCX: BN42_40170(kasB)
MCZ: BN45_50578(kasB)
MORY: MO_002355(kasB)
MLE: ML1656(kasB)
MLB: MLBr01656(kasB)
MPA: MAP_1999(kasB)
MAO: MAP4_1826
MAVI: RC58_09125
MAVU: RE97_09130
MAV: MAV_2191
MIT: OCO_21780
MIA: OCU_21960
MID: MIP_03076
MYO: OEM_19670
MCHI: AN480_11270
MIR: OCQ_20880
MMAL: CKJ54_09720
MLP: MLM_1920 MLM_3503
MMAN: MMAN_18030(kasB_1)
MSA: Mycsm_00734 Mycsm_03981
MUL: MUL_1303(kasB)
MMI: MMAR_3339(kasB)
MMAE: MMARE11_32400(kasB)
MLI: MULP_03600(kasB)
MPSE: MPSD_34160(kasB)
MSHO: MSHO_47510(kasB)
MMC: Mmcs_0783 Mmcs_3367
MKM: Mkms_0798 Mkms_3429
MJL: Mjls_0779 Mjls_3378
MMM: W7S_10240
MKN: MKAN_03350
MYV: G155_18580 G155_24920
MYE: AB431_04975 AB431_19265
MHAD: B586_09890
MDX: BTO20_06565 BTO20_13085
MSHG: MSG_03171(kasB_2)
MFJ: MFLOJ_00450(kasB_1)
MSTO: MSTO_01110(kasB_1)
MSIM: MSIM_45150(kasB_2)
MSAK: MSAS_12210(kasB_1) MSAS_12510(kasB_2)
MKU: I2456_16060 I2456_16415
MLW: MJO58_16835(kasB)
MXE: MYXE_20720(kasB) MYXE_43070
MNV: MNVI_22730 MNVI_45160(kasB)
MPAG: C0J29_07930 C0J29_18645
MNM: MNVM_02380(kasB)
MGOR: H0P51_17365 H0P51_25650
MCOO: MCOO_12830(kasB_1) MCOO_39080(kasB_2)
MBAI: MB901379_03011(kasB)
MSEO: MSEO_14160(kasB_1) MSEO_19580(kasB_2)
MHEK: JMUB5695_02081(kasB) JMUB5695_04192
MGAU: MGALJ_21320 MGALJ_52620
MLJ: MLAC_22600(kasB)
MBRD: MBRA_10910 MBRA_35610(kasB)
MSHJ: MSHI_10160(kasB)
MMAM: K3U93_14880
MSPG: F6B93_13970
MOT: LTS72_07815
MLM: MLPF_0931(kasB)
MPAA: MKK62_10055(kasB) MKK62_21435
MMEH: M5I08_06355 M5I08_12040(kasB)
MKY: IWGMT90018_24120 IWGMT90018_57110
MADI: A7U43_13130
MWU: PT015_07480(kasB) PT015_20985
MSM: MSMEG_1204 MSMEG_4328
MSG: MSMEI_1170(kasB) MSMEI_4228(kasB)
MSB: LJ00_05995 LJ00_21450
MSN: LI99_05995 LI99_21455
MSH: LI98_05995 LI98_21460
MVA: Mvan_3757
MGI: Mflv_2776
MSP: Mspyr1_22160
MCB: Mycch_3279
MNE: D174_18310
MYN: MyAD_17955
MFT: XA26_37940 XA26_50520
MPHL: MPHLCCUG_01036(kasB_1) MPHLCCUG_03393(kasB_2)
MVQ: MYVA_3579
MLL: B1R94_04715 B1R94_10585
MRH: MycrhN_0961 MycrhN_4434
MTHN: 4412656_01785(kasB)
MHAS: MHAS_00020(kasB_1) MHAS_01448(kasB_2)
MDU: MDUV_08130
MCHT: MCHIJ_33630
MDR: MDOR_04350 MDOR_24820
MAUU: NCTC10437_03514(kasB_1)
MMAG: MMAD_35140 MMAD_50950
MMOR: MMOR_29430 MMOR_56170
MFX: MFAL_13640
MAIC: MAIC_34220 MAIC_46930
MIJ: MINS_32350
MALV: MALV_02270
MTY: MTOK_56280
MPSC: MPSYJ_30180
MARZ: MARA_61340
MGAD: MGAD_02940 MGAD_51510
MHEV: MHEL_60260
MSAR: MSAR_35160
MANY: MANY_19080 MANY_48670
MAUB: MAUB_50710
MPOF: MPOR_39300
MPHU: MPHO_18700
MMUC: C1S78_009090
MMAT: MMAGJ_40560 MMAGJ_73570
MBOK: MBOE_35990
MFG: K6L26_13125 K6L26_26205
MSEI: MSEDJ_47250
MFLV: NCTC10271_01898(kasB_2) NCTC10271_04318(kasB_3)
MCEE: MCEL_03180 MCEL_25410
MBRM: L2Z93_001322
MMON: EWR22_04110 EWR22_17425
MGO: AFA91_18465 AFA91_32665
MHOL: K3U96_10645 K3U96_22490
MSEN: K3U95_05495 K3U95_16000
MPAE: K0O64_04540 K0O64_18630
MRF: MJO55_16905(kasB)
MDF: K0O62_18265
MCRO: MI149_05220 MI149_19455(kasB)
MGRO: FZ046_22650
MKR: MKOR_04700(kasB)
MPAK: MIU77_06595(kasB)
MJD: JDM601_1649(kasB) JDM601_2027(kasB)
MTER: 4434518_01562(kasB_1)
MMIN: MMIN_32490(kasB)
MHIB: MHIB_10830(kasB)
MHER: K3U94_09075
MVM: MJO54_09165(kasB)
 » show all
Reference
1  [PMID:11600501]
  Authors
Schaeffer ML, Agnihotri G, Volker C, Kallender H, Brennan PJ, Lonsdale JT
  Title
Purification and biochemical characterization of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthases KasA and KasB.
  Journal
J Biol Chem 276:47029-37 (2001)
DOI:10.1074/jbc.M108903200
  Sequence
[mtu:Rv2246]
Reference
2  [PMID:12950920]
  Authors
Gao LY, Laval F, Lawson EH, Groger RK, Woodruff A, Morisaki JH, Cox JS, Daffe M, Brown EJ
  Title
Requirement for kasB in Mycobacterium mycolic acid biosynthesis, cell wall impermeability and intracellular survival: implications for therapy.
  Journal
Mol Microbiol 49:1547-63 (2003)
DOI:10.1046/j.1365-2958.2003.03667.x
  Sequence
[mtu:Rv2246]
Reference
3  [PMID:16873379]
  Authors
Molle V, Brown AK, Besra GS, Cozzone AJ, Kremer L
  Title
The condensing activities of the Mycobacterium tuberculosis type II fatty acid synthase are differentially regulated by phosphorylation.
  Journal
J Biol Chem 281:30094-103 (2006)
DOI:10.1074/jbc.M601691200
  Sequence
[mtu:Rv2246]
Reference
4  [PMID:17360388]
  Authors
Bhatt A, Fujiwara N, Bhatt K, Gurcha SS, Kremer L, Chen B, Chan J, Porcelli SA, Kobayashi K, Besra GS, Jacobs WR Jr
  Title
Deletion of kasB in Mycobacterium tuberculosis causes loss of acid-fastness and subclinical latent tuberculosis in immunocompetent mice.
  Journal
Proc Natl Acad Sci U S A 104:5157-62 (2007)
DOI:10.1073/pnas.0608654104
Reference
5  [PMID:22516756]
  Authors
Yamada H, Bhatt A, Danev R, Fujiwara N, Maeda S, Mitarai S, Chikamatsu K, Aono A, Nitta K, Jacobs WR Jr, Nagayama K
  Title
Non-acid-fastness in Mycobacterium tuberculosis DeltakasB mutant correlates with  the cell envelope electron density.
  Journal
Tuberculosis (Edinb) 92:351-7 (2012)
DOI:10.1016/j.tube.2012.02.006
Reference
6  [PMID:24809459]
  Authors
Vilcheze C, Molle V, Carrere-Kremer S, Leiba J, Mourey L, Shenai S, Baronian G, Tufariello J, Hartman T, Veyron-Churlet R, Trivelli X, Tiwari S, Weinrick B, Alland D, Guerardel Y, Jacobs WR Jr, Kremer L
  Title
Phosphorylation of KasB regulates virulence and acid-fastness in Mycobacterium tuberculosis.
  Journal
PLoS Pathog 10:e1004115 (2014)
DOI:10.1371/journal.ppat.1004115
  Sequence
[mtu:Rv2246]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.294
IUBMB Enzyme Nomenclature: 2.3.1.294
ExPASy - ENZYME nomenclature database: 2.3.1.294
BRENDA, the Enzyme Database: 2.3.1.294

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Chemical substance (3)   
   KEGG COMPOUND (3)   
Gene (212)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (211)   
Protein sequence (8)   
   UniProt (4)   
   SWISS-PROT (4)   
Protein domain (5)   
   InterPro (5)   
All databases (228)   

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