KEGG   ENZYME: 2.3.1.301
Entry
EC 2.3.1.301                Enzyme                                 
Name
mycobacterial beta-ketoacyl-[acyl carrier protein] synthase III;
fabH (gene name) (ambiguous);
mycobacterial 3-oxoacyl-[acyl carrier protein] synthase III
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
dodecanoyl-CoA:malonyl-[acyl-carrier protein] C-acyltransferase
Reaction(IUBMB)
dodecanoyl-CoA + a malonyl-[acyl-carrier protein] = a 3-oxotetradecanoyl-[acyl-carrier protein] + CoA + CO2 [RN:R12735]
Reaction(KEGG)
R12735;
(other) R13257
Substrate
dodecanoyl-CoA [CPD:C01832];
malonyl-[acyl-carrier protein] [CPD:C01209]
Product
3-oxotetradecanoyl-[acyl-carrier protein] [CPD:C05759];
CoA [CPD:C00010];
CO2 [CPD:C00011]
Comment
The enzyme, characterized from mycobacteria, provides a link between the type I and type II fatty acid synthase systems (FAS-I and FAS-II, respectively) found in these organisms. The enzyme acts on medium- and long-chain acyl-CoAs (C12-C16) produced by the FAS-I system, condensing them with malonyl-[acyl-carrier protein] (malonyl-AcpM) and forming starter molecules for the FAS-II system, which elongates them into meromycolic acids. The enzyme has no activity with short-chain acyl-CoAs (e.g. acetyl-CoA), which are used by EC 2.3.1.180, beta-ketoacyl-[acyl-carrier-protein] synthase III, or branched-chain acyl-CoAs, which are used by EC 2.3.1.300, branched-chain beta-ketoacyl-[acyl-carrier-protein] synthase.
History
EC 2.3.1.301 created 2021
Orthology
K11608  mycobacterial beta-ketoacyl-[acyl-carrier-protein] synthase III
Genes
BQB: J4P68_0030340
MTU: Rv0533c(fabH)
MTV: RVBD_0533c
MTC: MT0557(fabH)
MRA: MRA_0540(fabH)
MTF: TBFG_10544
MTB: TBMG_00539
MTK: TBSG_00545
MTZ: TBXG_000536
MTG: MRGA327_03370
MTI: MRGA423_03345
MTUR: CFBS_0557(fabH)
MTO: MTCTRI2_0541(fabH)
MTD: UDA_0533c(fabH)
MTJ: J112_02855
MTUB: MT7199_0547
MTUC: J113_03805
MTUE: J114_02855
MTX: M943_02765
MTUH: I917_03830
MTUL: TBHG_00531
MTUT: HKBT1_0557(fabH)
MTUU: HKBT2_0557(fabH)
MTQ: HKBS1_0557(fabH)
MBO: BQ2027_MB0547C(fabH)
MBB: BCG_0577c(fabH)
MBT: JTY_0547(fabH)
MBM: BCGMEX_0548c(fabH)
MBK: K60_005660
MBX: BCGT_0317
MAF: MAF_05400(fabH)
MMIC: RN08_0596
MCE: MCAN_05361(fabH)
MCQ: BN44_10593(fabH)
MCV: BN43_10584(fabH)
MCX: BN42_20271(fabH)
MCZ: BN45_10598(fabH)
MORY: MO_000565(fabH)
MPA: MAP_4028c(fabH)
MAO: MAP4_4151
MAVI: RC58_20595
MAVU: RE97_20645
MAV: MAV_4612
MIT: OCO_44980
MIA: OCU_44750
MID: MIP_06835
MYO: OEM_45190
MCHI: AN480_23535
MIR: OCQ_46100
MMAL: CKJ54_21430
MLP: MLM_3777
MMAN: MMAN_33270(fabH_2)
MUL: MUL_0632(fabH)
MMI: MMAR_0879(fabH)
MMAE: MMARE11_08290(fabH)
MLI: MULP_00907(fabH)
MPSE: MPSD_10100(fabH)
MSHO: MSHO_26380(fabH)
MMM: W7S_22645
MKN: MKAN_18700
MSHG: MSG_00757(fabH)
MFJ: MFLOJ_27180(fabH)
MSTO: MSTO_31080(fabH)
MSIM: MSIM_17160(fabH)
MKU: I2456_04475
MLW: MJO58_04045
MPAG: C0J29_04745
MGOR: H0P51_04385
MBAI: MB901379_00748(fabH)
MSEO: MSEO_46770(fabH)
MLJ: MLAC_36200(fabH)
MBRD: MBRA_09820(fabH)
MSHJ: MSHI_29050(fabH)
MSPG: F6B93_03570(fabH)
MOT: LTS72_21600
MKY: IWGMT90018_08660(fabH)
MFX: MFAL_31900(fabH)
MIJ: MINS_11360(fabH)
MAUB: MAUB_45000(fabH)
MPHU: MPHO_24330(fabH)
MMUC: C1S78_006165
MKR: MKOR_23540(fabH)
MPAK: MIU77_15455
MAB: MAB_1141c
MMV: MYCMA_0605
MABB: MASS_1137
MABL: MMASJCM_1150
MIZ: BAB75_06060
MSTE: MSTE_01096(fabH)
MSAO: MYCSP_04930
MSAL: DSM43276_00997(fabH_1)
MMIN: MMIN_21150(fabH)
MHIB: MHIB_39220(fabH)
MHER: K3U94_02295
MVM: MJO54_02050
ASD: AS9A_2905(fabH) AS9A_2908(fabH3)
NFA: NFA_52820(fabH) NFA_53970(fabH3)
NFR: ERS450000_04471(fabH_4) ERS450000_04599(fabH_5) ERS450000_04925(fabH_6)
NCY: NOCYR_1634(fabH) NOCYR_5108(fabH) NOCYR_5244(fabH)
NBR: O3I_037595 O3I_039605 O3I_040210
NNO: NONO_c71510(fabH1) NONO_c72620(fabH2)
NSL: BOX37_05545 BOX37_30280 BOX37_30855
NSR: NS506_00291 NS506_01121 NS506_05990
NTP: CRH09_37520 CRH09_38000
NOZ: DMB37_18410 DMB37_19015
NOD: FOH10_19325 FOH10_33185 FOH10_33695
NAH: F5544_42075(fabH) F5544_42770(fabH)
NAD: NCTC11293_00255(fabH_1) NCTC11293_00382(fabH_2) NCTC11293_02068(fabH_4)
NWL: NWFMUON74_49000(fabH1) NWFMUON74_67100(fabH_2) NWFMUON74_68100(fabH_3)
NIE: KV110_36795 KV110_38765 KV110_39345
NTC: KHQ06_03950 KHQ06_04480 KHQ06_30995
NHU: H0264_10545 H0264_37245 H0264_37850
NYA: LTV02_04610 LTV02_33155 LTV02_33715
NGP: LTT66_09560 LTT66_10155
NSPU: IFM12276_59140(fabH_1) IFM12276_60300(fabH_2)
NYU: D7D52_27040 D7D52_29385 D7D52_31335(fabH)
RHA: RHA1_ro05206
RER: RER_11360(fabH)
REY: O5Y_05155
REB: XU06_05400
RQI: C1M55_05605
ROP: ROP_52560(fabH)
ROA: Pd630_LPD01731
RPY: Y013_11460
RBY: CEJ39_22365
RHB: NY08_3715
RAV: AAT18_22395
RFA: A3L23_01861(fabH)
RHS: A3Q41_01504(fabH)
RHW: BFN03_00840
RRZ: CS378_12215
RHU: A3Q40_02272(fabH)
RHQ: IM25_03165
RHOD: AOT96_16005
RRT: 4535765_04192(fabH_2)
RCR: NCTC10994_00252(fabB_1) NCTC10994_03167(fabH)
RTM: G4H71_06280
RKO: JWS14_28525 JWS14_39915
RGO: KYT97_13280
ROZ: CBI38_25285
RPSK: JWS13_16060
RGOR: NMQ04_18240
RHOP: D8W71_03360
REQ: REQ_28490(fabB) REQ_40910(fabH)
PDEF: P9209_07030
WHR: OG579_12070 OG579_20335
GBR: Gbro_1530 Gbro_1536
GPO: GPOL_c13590(fabH1) GPOL_c13640(fabH2)
GOR: KTR9_1451 KTR9_1454
GOQ: ACH46_04630 ACH46_04660
GTA: BCM27_08040 BCM27_08055
GOC: CXX93_17340 CXX93_17380
GIT: C6V83_05515 C6V83_05535
GRU: GCWB2_07615(fabH2)
GOM: D7316_01743(fabH_2)
GAV: C5O27_20195
GOD: GKZ92_07235(fabH)
GAM: GII34_06710(fabH) GII34_10700(fabH)
GPD: GII33_06160(fabH) GII33_10350(fabH)
GJI: H1R19_07445 H1R19_07460
GOI: LK459_03245 LK459_03250
GHN: MVF96_07450 MVF96_07470
GAMI: IHQ52_10380
GMG: NWF22_18095 NWF22_18100
GSI: P5P27_03525 P5P27_03565
SPIN: KV203_02315 KV203_02375
TPR: Tpau_2193
TSM: ASU32_11935
TPUL: TPB0596_23890(fabH1)
TSD: MTP03_24850(fabH1)
DTM: BJL86_3010
DIT: C3V38_03975
DIZ: CT688_14605
DPC: A6048_15280
DLU: A6035_14580
DKN: NHB83_14780
TOY: FO059_01830
LMOI: VV02_03980
NSN: EXE58_08650
NOQ: LN652_12270
AMYY: YIM_23715(fabH2)
 » show all
Reference
1  [PMID:11278743]
  Authors
Scarsdale JN, Kazanina G, He X, Reynolds KA, Wright HT.
  Title
Crystal structure of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III.
  Journal
J Biol Chem 276:20516-22 (2001)
DOI:10.1074/jbc.M010762200
  Sequence
[mtu:Rv0533c]
Reference
2  [PMID:15713483]
  Authors
Musayev F, Sachdeva S, Scarsdale JN, Reynolds KA, Wright HT.
  Title
Crystal structure of a substrate complex of Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III (FabH) with lauroyl-coenzyme A.
  Journal
J Mol Biol 346:1313-21 (2005)
DOI:10.1016/j.jmb.2004.12.044
  Sequence
[mtu:Rv0533c]
Reference
3  [PMID:16040614]
  Authors
Brown AK, Sridharan S, Kremer L, Lindenberg S, Dover LG, Sacchettini JC, Besra GS.
  Title
Probing the mechanism of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III mtFabH: factors influencing catalysis and substrate specificity.
  Journal
J Biol Chem 280:32539-47 (2005)
DOI:10.1074/jbc.M413216200
  Sequence
[mtu:Rv0533c]
Reference
4  [PMID:18420147]
  Authors
Sachdeva S, Musayev FN, Alhamadsheh MM, Scarsdale JN, Wright HT, Reynolds KA.
  Title
Separate entrance and exit portals for ligand traffic in Mycobacterium tuberculosis FabH.
  Journal
Chem Biol 15:402-12 (2008)
DOI:10.1016/j.chembiol.2008.03.007
  Sequence
[mtu:Rv0533c]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.301
IUBMB Enzyme Nomenclature: 2.3.1.301
ExPASy - ENZYME nomenclature database: 2.3.1.301
BRENDA, the Enzyme Database: 2.3.1.301

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Chemical substance (7)   
   KEGG COMPOUND (7)   
Chemical reaction (4)   
   KEGG REACTION (2)   
   KEGG RCLASS (2)   
Gene (235)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (234)   
Protein sequence (165)   
   UniProt (155)   
   SWISS-PROT (10)   
Protein domain (4)   
   InterPro (4)   
All databases (415)   

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