KEGG   ENZYME: 2.3.2.12
Entry
EC 2.3.2.12                 Enzyme                                 
Name
peptidyltransferase;
transpeptidase;
ribosomal peptidyltransferase
Class
Transferases;
Acyltransferases;
Aminoacyltransferases
Sysname
peptidyl-tRNA:aminoacyl-tRNA N-peptidyltransferase
Reaction(IUBMB)
peptidyl-tRNA1 + aminoacyl-tRNA2 = tRNA1 + peptidyl(aminoacyl-tRNA2) [RN:R04685]
Reaction(KEGG)
R04685
Substrate
peptidyl-tRNA1;
aminoacyl-tRNA2
Product
tRNA1;
peptidyl(aminoacyl-tRNA2)
Comment
The enzyme is a ribozyme. Two non-equivlant ribonucleoprotein subunits operate in non-concerted fashion in peptide elongation. The small subunit forms the mRNA-binding machinery and decoding center, the large subunit performs the main ribosomal catalytic function in the peptidyl-transferase center.
History
EC 2.3.2.12 created 1976
Reference
1  [PMID:5329275]
  Authors
Rychlik I.
  Title
Release of lysine peptides by puromycin from polylysyl-transfer ribonucleic acid in the presence of ribosomes.
  Journal
Biochim Biophys Acta 114:425-7 (1966)
DOI:10.1016/0005-2787(66)90327-3
Reference
2  [PMID:4897787]
  Authors
Rychlik I, Cerna J, Chladek S, Zemlicka J, Haladova Z.
  Title
Substrate specificity of ribosomal peptidyl transferase: 2'(3')-O-aminoacyl nucleosides as acceptors of the peptide chain on the amino acid site.
  Journal
J Mol Biol 43:13-24 (1969)
DOI:10.1016/0022-2836(69)90075-8
Reference
3  [PMID:14222897]
  Authors
TRAUT RR, MONRO RE
  Title
THE PUROMYCIN REACTION AND ITS RELATION TO PROTEIN SYNTHESIS.
  Journal
J Mol Biol 10:63-72 (1964)
DOI:10.1016/S0022-2836(64)80028-0
Reference
4  [PMID:19363482]
  Authors
Voorhees RM, Weixlbaumer A, Loakes D, Kelley AC, Ramakrishnan V
  Title
Insights into substrate stabilization from snapshots of the peptidyl transferase  center of the intact 70S ribosome.
  Journal
Nat Struct Mol Biol 16:528-33 (2009)
DOI:10.1038/nsmb.1577
Other DBs
ExplorEnz - The Enzyme Database: 2.3.2.12
IUBMB Enzyme Nomenclature: 2.3.2.12
ExPASy - ENZYME nomenclature database: 2.3.2.12
BRENDA, the Enzyme Database: 2.3.2.12
CAS: 9059-29-4

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Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (3)   
   KEGG REACTION (1)   
   KEGG RCLASS (2)   
Protein sequence (22)   
   UniProt (6)   
   PDBSTR (15)   
   PMD (1)   
3D Structure (9)   
   PDB (9)   
All databases (38)   

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