KEGG ENZYME: 2.3.2.17

ENZYME: 2.3.2.17

Entry

EC 2.3.2.17                 Enzyme

Name

N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase;
femA (gene name);
N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-ditrans,octacis-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase

Class

Transferases;
Acyltransferases;
Aminoacyltransferases

Sysname

MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc:glycine glycyltransferase

Reaction(IUBMB)

MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 glycyl-tRNAGly = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 tRNAGly [RN:R08777]

Reaction(KEGG)

R08777

Substrate

MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc;
glycyl-tRNA(Gly) [CPD:C02412]

Product

MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc;
tRNA(Gly) [CPD:C01642]

Comment

This enzyme catalyses the successive transfer of two Gly moieties from charged tRNAs to MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc, attaching them to a Gly residue previously attached by EC 2.3.2.16 (lipid II:glycine glycyltransferase) to the N6 of the L-Lys at position 3 of the pentapeptide. This is the second step in the synthesis of the pentaglycine interpeptide bridge that is used by Staphylococcus aureus for the crosslinking of different glycan strands to each other. The next step is catalysed by EC 2.3.2.18 (N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase). This enzyme is essential for methicillin resistance [1].

History

EC 2.3.2.17 created 2010

Pathway

ec00550

Peptidoglycan biosynthesis

ec01100

Metabolic pathways

Orthology

K11694

peptidoglycan pentaglycine glycine transferase (the second and third glycine)

Genes

SDO:	SD1155_09025

SAU:	SA1206(femA)

SAV:	SAV1374(femA)

SAW:	SAHV_1362(femA)

SAH:	SaurJH1_1464

SAJ:	SaurJH9_1435

SAM:	MW1261(femA)

SAS:

SAS1314

SAR:	SAR1387(femA)

SAC:	SACOL1410(femA)

SAX:	USA300HOU_1309(femA)

SAA:	SAUSA300_1269(femA)

SAO:	SAOUHSC_01373

SAE:	NWMN_1286(femA)

SAD:	SAAV_1356(femA)

SUU:	M013TW_1320(femA)

SUV:	SAVC_06120

SUE:

SAOV_1384

SUJ:	SAA6159_01241(femA)

SUK:	SAA6008_01339(femA)

SUC:	ECTR2_1231

SUT:	SAT0131_01446

SUQ:	HMPREF0772_11833

SUZ:

MS7_1331

SUD:	ST398NM01_1375

SUX:	SAEMRSA15_12220(femA)

SUW:	SATW20_13740(femA)

SUG:

SAPIG1375

SUF:	SARLGA251_12830(femA)

SAUA:

SAAG_01984

SAUE:

RSAU_001255(femA)

SAUN:

SAKOR_01311

SAUS:

SA40_1251(femA)

SAUU:

SA957_1266(femA)

SAUG:

SA268_1271(femA)

SAUZ:

SAZ172_1385(femA)

SAUT:

SAI1T1_2009890

SAUJ:

SAI2T2_1009910

SAUK:

SAI3T3_1009900

SAUQ:

SAI4T8_1009890

SAUV:

SAI7S6_1009900

SAUW:

SAI5S5_1009860

SAUX:

SAI6T6_1009870

SAUY:

SAI8T7_1009900

SAUF:

X998_1382(femA)

SAB:	SAB1229(femA)

SUY:	SA2981_1328(femA)

SAUB:

C248_1412(femA)

SAUM:

BN843_12880

SAUC:

CA347_1311(femA)

SAUR:

SABB_00138(femA)

SAUI:

AZ30_06695

SAUD:

CH52_12195

SAMS:

NI36_06780

SUH:	SAMSHR1132_12170

SER:	SERP0946(femA)

SEP:

SE_1057

SEPP:

SEB_01083

SEPS:

DP17_11(femA)

SHA:	SH1532(femA)

SHH:	ShL2_01411(femA)

SSP:

SSP1371

SCA:	SCA_1019(femA)

SLG:	SLGD_01472

SLN:	SLUG_14700(femA)

SSD:	SPSINT_1097

SDT:	SPSE_1403(femA)

SDP:	NCTC12225_01613(femA)

SWA:	A284_06765

SPAS:

STP1_2404(femA2)

SXY:	BE24_04240

SXL:	SXYLSMQ121_1409

SXO:	SXYL_01495(femA)

SHU:	SHYC_07545(femA)

SCAP:

AYP1020_0683(femA_1)

SSCH:

LH95_06785

SSCZ:

RN70_07255

SAGQ:

EP23_02880

SEQO:

SE1039_12200

SSIF:

AL483_04840

SCV:	A4G25_02380

SPET:

CEP67_01500

SLZ:	B5P37_11050

SCOH:

BZ166_11435

SNL:	BJD96_07455

SKL:	C7J89_08935

SFQ:	C7J90_08605

SHOM:

EGX58_10985

SMUS:

C7J88_02970

SCAR:

DWB96_07505

SCHR:

DWB92_07045

SARL:

SAP2_14560(femA)

SPIC:

SAMEA4384060_1557(femA_2)

SSH:	NCTC13712_01367(femA_2)

SSIM:

SAMEA4384339_1273(femA_1)

SDB:	CNQ82_06930

SLLO:

ISP08_07250

SAUL:

I6G39_06610

SSAC:

I6I31_08955

SRAI:

LN051_06405

SPSD:

JMB28_07770

STAS:

HYI43_07115

SURY:

MUA21_05655

SEDA:

MNY58_07660

SCAQ:

QMO72_07625

SDEV:

Q2T90_00235

SROT:

ML435_06375(femA)

STAP:

AOB58_555

SSCU:

CEP64_06430

SFF:	FOB90_00655

SSTE:

SAMEA4384403_1395(femA_2)

MLEN:

H3V22_01675

MVT:	I6J10_04355

MACR:

BHM04_03940

MARY:

LAU42_05600

MBRU:

MGG13_06055

MEPI:

KFV12_07170

MEQU:

KFV11_05715

MBOC:

QSV55_05670

MCL:	MCCL_1041(femA)

MCAK:

MCCS_12180(femA_2)

MBOE:

HT586_06620

SHV:	AAT16_07830

JEA:	JEM45_01585

NAMP:

KPF49_04530

NMY:	CJ229_002290

SBAC:

BVH56_04465

» show all

Reference

1 [PMID:2559314]

Authors

Berger-Bachi B, Barberis-Maino L, Strassle A, Kayser FH

Title

FemA, a host-mediated factor essential for methicillin resistance in Staphylococcus aureus: molecular cloning and characterization.

Journal

Mol Gen Genet 219:263-9 (1989)
DOI:10.1007/BF00261186

Sequence

[sau:SA1206]

Reference

2 [PMID:7590176]

Authors

Johnson S, Kruger D, Labischinski H

Title

FemA of Staphylococcus aureus: isolation and immunodetection.

Journal

FEMS Microbiol Lett 132:221-8 (1995)
DOI:10.1111/j.1574-6968.1995.tb07837.x

Sequence

[sao:SAOUHSC_01373]

Reference

3 [PMID:12176388]

Authors

Benson TE, Prince DB, Mutchler VT, Curry KA, Ho AM, Sarver RW, Hagadorn JC, Choi GH, Garlick RL

Title

X-ray crystal structure of Staphylococcus aureus FemA.

Journal

Structure 10:1107-15 (2002)
DOI:10.1016/S0969-2126(02)00807-9

Sequence

[saa:SAUSA300_1269]

Reference

4 [PMID:15228543]

Authors

Schneider T, Senn MM, Berger-Bachi B, Tossi A, Sahl HG, Wiedemann I

Title

In vitro assembly of a complete, pentaglycine interpeptide bridge containing cell wall precursor (lipid II-Gly5) of Staphylococcus aureus.

Journal

Mol Microbiol 53:675-85 (2004)
DOI:10.1111/j.1365-2958.2004.04149.x

Sequence

[sao:SAOUHSC_01373]

Other DBs

ExplorEnz - The Enzyme Database:

2.3.2.17

IUBMB Enzyme Nomenclature:

2.3.2.17

ExPASy - ENZYME nomenclature database:

2.3.2.17

BRENDA, the Enzyme Database:

2.3.2.17

DBGET integrated database retrieval system