KEGG ENZYME: 2.3.2.18

ENZYME: 2.3.2.18

Entry

EC 2.3.2.18                 Enzyme

Name

N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase;
femB (gene name);
N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-ditrans,octacis-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase

Class

Transferases;
Acyltransferases;
Aminoacyltransferases

Sysname

MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc:glycine glycyltransferase

Reaction(IUBMB)

MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 glycyl-tRNAGly = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-penta-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 tRNAGly [RN:R08778]

Reaction(KEGG)

R08778

Substrate

MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc;
glycyl-tRNA(Gly) [CPD:C02412]

Product

MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-penta-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc;
tRNA(Gly) [CPD:C01642]

Comment

This Staphylococcus aureus enzyme catalyses the successive transfer of two Gly moieties from charged tRNAs to MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphosphoundecaprenyl-GlcNAc, attaching them to the three Gly molecules that were previously attached to the N6 of the L-Lys at position 3 of the pentapeptide by EC 2.3.2.16 (lipid II:glycine glycyltransferase) and EC 2.3.2.17 (N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase). This is the last step in the synthesis of the pentaglycine interpeptide bridge that is used in this organism for the crosslinking of different glycan strands to each other.

History

EC 2.3.2.18 created 2010

Pathway

ec00550

Peptidoglycan biosynthesis

ec01100

Metabolic pathways

Orthology

K11695

peptidoglycan pentaglycine glycine transferase (the fourth and fifth glycine)

Genes

SDO:	SD1155_09030

SAU:	SA1207(femB)

SAV:	SAV1375(femB)

SAW:	SAHV_1363(femB)

SAH:	SaurJH1_1465

SAJ:	SaurJH9_1436

SAM:	MW1262(femB)

SAS:

SAS1315

SAR:	SAR1388(femB)

SAC:

SACOL1411

SAX:	USA300HOU_1310(femB)

SAA:	SAUSA300_1270(femB)

SAO:	SAOUHSC_01374

SAE:	NWMN_1287(femB)

SAD:

SAAV_1357

SUU:	M013TW_1321(femB)

SUV:	SAVC_06125

SUE:

SAOV_1385

SUJ:	SAA6159_01242(femB)

SUK:	SAA6008_01340(femB)

SUC:	ECTR2_1232

SUT:	SAT0131_01447

SUQ:	HMPREF0772_11832(femB)

SUZ:	MS7_1332(femB)

SUD:	ST398NM01_1376

SUX:	SAEMRSA15_12230(femB)

SUW:	SATW20_13750(femB)

SUG:

SAPIG1376

SUF:	SARLGA251_12840(femB)

SAUA:

SAAG_01985

SAUE:

RSAU_001256(femB)

SAUN:

SAKOR_01312

SAUS:

SA40_1252(femB)

SAUU:

SA957_1267(femB)

SAUG:

SA268_1272(femB)

SAUZ:

SAZ172_1386(femB)

SAUT:

SAI1T1_2009900(femB)

SAUJ:

SAI2T2_1009920(femB)

SAUK:

SAI3T3_1009910(femB)

SAUQ:

SAI4T8_1009900(femB)

SAUV:

SAI7S6_1009910(femB)

SAUW:

SAI5S5_1009870(femB)

SAUX:

SAI6T6_1009880(femB)

SAUY:

SAI8T7_1009910(femB)

SAUF:

X998_1383(femB)

SAB:	SAB1230(femB)

SUY:	SA2981_1329(femB)

SAUB:

C248_1413(femB)

SAUM:

BN843_12890

SAUC:

CA347_1312(femB)

SAUR:

SABB_00137(femB)

SAUI:

AZ30_06700

SAUD:

CH52_12190

SAMS:

NI36_06785

SUH:	SAMSHR1132_12180

SER:	SERP0947(femB)

SEP:

SE_1058

SEPP:

SEB_01084

SEPS:

DP17_12(femB)

SHA:	SH1531(femB)

SHH:	ShL2_01410(femB)

SSP:

SSP1370

SCA:	SCA_1020(femB)

SLG:	SLGD_01473

SLN:	SLUG_14710(femB)

SSD:	SPSINT_1098

SDT:	SPSE_1402(femB)

SDP:	NCTC12225_01612(femB)

SWA:	A284_06760

SPAS:

STP1_2405(femB)

SXY:	BE24_04245

SXL:	SXYLSMQ121_1408

SXO:	SXYL_01494(femB)

SHU:	SHYC_07540(femB-2)

SCAP:

AYP1020_0684(femB)

SSCH:

LH95_06780

SSCZ:

RN70_07250

SAGQ:

EP23_02885

SEQO:

SE1039_12210

SSIF:

AL483_04835

SCV:	A4G25_02375

SPET:

CEP67_01505

SLZ:	B5P37_11055

SNL:	BJD96_07450

SKL:	C7J89_08930

SFQ:	C7J90_08610

SHOM:

EGX58_10980

SMUS:

C7J88_02965

SCAR:

DWB96_07500

SCHR:

DWB92_07040

SARL:

SAP2_14550(femB)

SPIC:

SAMEA4384060_1556(femB)

SSH:	NCTC13712_01368(femB)

SSIM:

SAMEA4384339_1274(femB)

SDB:	CNQ82_06935

SLLO:

ISP08_07245

SAUL:

I6G39_06605

SSAC:

I6I31_08960

SRAI:

LN051_06400

SPSD:

JMB28_07765

STAS:

HYI43_07110

SURY:

MUA21_05660

SEDA:

MNY58_07655

SCAQ:

QMO72_07620

SDEV:

Q2T90_00240

SROT:

ML435_06380(femB)

STAP:

AOB58_554

SSCU:

CEP64_06425

SFF:	FOB90_00660

SSTE:

SAMEA4384403_1394(femB)

MLEN:

H3V22_01680

MVT:	I6J10_04360

MARY:

LAU42_05605

MBRU:

MGG13_06060

MEPI:

KFV12_07175

MEQU:

KFV11_05720

MBOC:

QSV55_05675

MCL:	MCCL_1042(femB)

MCAK:

MCCS_12190(femB)

MBOE:

HT586_06625

SHV:	AAT16_07825

JEA:	JEM45_01590

NAMP:

KPF49_04525

NMY:	CJ229_002285

» show all

Reference

1 [PMID:9393725]

Authors

Ehlert K, Schroder W, Labischinski H

Title

Specificities of FemA and FemB for different glycine residues: FemB cannot substitute for FemA in staphylococcal peptidoglycan pentaglycine side chain formation.

Journal

J Bacteriol 179:7573-6 (1997)
DOI:10.1128/JB.179.23.7573-7576.1997

Sequence

[sao:SAOUHSC_01374]

Reference

2 [PMID:14523106]

Authors

Rohrer S, Berger-Bachi B

Title

Application of a bacterial two-hybrid system for the analysis of protein-protein interactions between FemABX family proteins.

Journal

Microbiology 149:2733-8 (2003)
DOI:10.1099/mic.0.26315-0