Entry Name cyclo(L-leucyl-L-phenylalanyl) synthase;
Class Transferases;BRITE hierarchy
Sysname L-leucyl-tRNALeu:L-phenylalanyl-tRNAPhe leucyltransferase (cyclizing)
Reaction(IUBMB) L-leucyl-tRNALeu + L-phenylalanyl-tRNAPhe = tRNALeu + tRNAPhe + cyclo(L-leucyl-L-phenylalanyl) [RN:
R10334 ]
Reaction(KEGG) Substrate L-leucyl-tRNA(Leu) [CPD:
C02047 ];
L-phenylalanyl-tRNA(Phe) [CPD:
C03511 ]
Product tRNA(Leu) [CPD:
C01645 ];
tRNA(Phe) [CPD:
C01648 ];
cyclo(L-leucyl-L-phenylalanyl) [CPD:
C20519 ]
Comment The reaction proceeds following a ping-pong mechanism forming a covalent intermediate between an active site serine and the L-phenylalanine residue [2]. The protein, found in the bacterium Streptomyces noursei, also forms cyclo(L-phenylalanyl-L-phenylalanyl), cyclo(L-methionyl-L-phenylalanyl), cyclo(L-phenylalanyl-L-tyrosyl) and cyclo(L-methionyl-L-tyrosyl) [1].
History EC 2.3.2.20 created 2013
Orthology K21382 cyclo(L-leucyl-L-phenylalanyl) synthase
Genes Taxonomy Reference Authors Gondry M, Sauguet L, Belin P, Thai R, Amouroux R, Tellier C, Tuphile K, Jacquet M, Braud S, Courcon M, Masson C, Dubois S, Lautru S, Lecoq A, Hashimoto S, Genet R, Pernodet JL
Title Cyclodipeptide synthases are a family of tRNA-dependent peptide bond-forming enzymes.
Journal Sequence Reference Authors Sauguet L, Moutiez M, Li Y, Belin P, Seguin J, Le Du MH, Thai R, Masson C, Fonvielle M, Pernodet JL, Charbonnier JB, Gondry M
Title Cyclodipeptide synthases, a family of class-I aminoacyl-tRNA synthetase-like enzymes involved in non-ribosomal peptide synthesis.
Journal Sequence Other DBs ExplorEnz - The Enzyme Database: 2.3.2.20
ExPASy - ENZYME nomenclature database: 2.3.2.20
