KEGG ENZYME: 2.3.2.22

ENZYME: 2.3.2.22

Entry

EC 2.3.2.22                 Enzyme

Name

cyclo(L-leucyl-L-leucyl) synthase;
YvmC;
cLL synthase;
cyclodileucine synthase

Class

Transferases;
Acyltransferases;
Aminoacyltransferases

Sysname

L-leucyl-tRNALeu:L-leucyl-tRNALeu leucyltransferase (cyclizing)

Reaction(IUBMB)

2 L-leucyl-tRNALeu = 2 tRNALeu + cyclo(L-leucyl-L-leucyl) [RN:R10336]

Reaction(KEGG)

R10336

Substrate

L-leucyl-tRNA(Leu) [CPD:C02047]

Product

tRNA(Leu) [CPD:C01645];
cyclo(L-leucyl-L-leucyl) [CPD:C20514]

Comment

The reaction proceeds following a ping-pong mechanism forming a covalent intermediate between an active site serine and the first L-leucine residue [2]. The proteins from bacteria of the genus Bacillus also form small amounts of cyclo(L-phenylalanyl-L-leucyl) and cyclo(L-leucyl-L-methionyl) [1].

History

EC 2.3.2.22 created 2013

Orthology

K17485

cyclo(L-leucyl-L-leucyl) synthase

Genes

PLU:

plu0297

PLUM:

A4R40_01450

PAKH:

B0X70_01355

PLUI:

CE143_01355

XGR:	QL128_02115

MME:	Marme_0276

BSU:	BSU35070(yvmC)

BSL:

A7A1_1219

BSH:	BSU6051_35070(yvmC)

BSUT:

BSUB_03737

BSUL:

BSUA_03737(yvmC)

BSUS:

Q433_19195

BSQ:	B657_35070

BSP:	U712_17565

BSS:	BSUW23_17190(yvmC)

BST:

GYO_3844

BIT:	BIS30_07160

BLI:	BL00817(yvmC)

BLD:	BLi03566(yvmC)

BLH:	BaLi_c36280(yvmC)

BMOJ:

HC660_33920(pchC)

BBY:	CY96_28930

BRY:	M0696_17755(pchC)

BACY:

QF06_15920

BACL:

BS34A_38170(yvmC)

BALM:

BsLM_3514

BGI:	BGM20_11665

ACAI:

ISX45_01290

SEPS:

DP17_2385

SHA:

pSHaeC06

SSD:	SPSINT_1708

SDT:

SPSE_0787

SDP:	NCTC12225_00487

CJK:

jk0923

SGM:	GCM10017557_58260(yvmC)

SSUB:

CP968_01305

SNIG:

HEK616_79620(yvmC)

SHAU:

K9S39_11150

CROS:

N8J89_05045

» show all

Reference

1 [PMID:19430487]

Authors

Gondry M, Sauguet L, Belin P, Thai R, Amouroux R, Tellier C, Tuphile K, Jacquet M, Braud S, Courcon M, Masson C, Dubois S, Lautru S, Lecoq A, Hashimoto S, Genet R, Pernodet JL

Title

Cyclodipeptide synthases are a family of tRNA-dependent peptide bond-forming enzymes.

Journal

Nat Chem Biol 5:414-20 (2009)
DOI:10.1038/nchembio.175

Sequence

[bsu:BSU35070] [plu:plu0297]

Reference

2 [PMID:21325056]

Authors

Bonnefond L, Arai T, Sakaguchi Y, Suzuki T, Ishitani R, Nureki O

Title

Structural basis for nonribosomal peptide synthesis by an aminoacyl-tRNA synthetase paralog.