Entry
Name
(E3-independent) E2 ubiquitin-conjugating enzyme;
E2-230K;
UBE2O;
E3-independent ubiquitin-conjugating enzyme E2
Class
Transferases;
Acyltransferases;
Aminoacyltransferases
BRITE hierarchy
Sysname
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine:L-lysine ubiquitinyl transferase ([E3 ubiquitin transferase]-independent)
Reaction(IUBMB)
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine (overall reaction);
(1a) [E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + [(E3-independent) ubiquitin-conjugating enzyme]-S-monoubiquitinyl-L-cysteine;
(1b) [(E3-independent) E2 ubiquitin-conjugating E2 enzyme]-S-monoubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine
Substrate
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine;
[acceptor protein]-L-lysine;
[(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine;
[(E3-independent) E2 ubiquitin-conjugating E2 enzyme]-S-monoubiquitinyl-L-cysteine
Product
[E1 ubiquitin-activating enzyme]-L-cysteine;
[acceptor protein]-N6-monoubiquitinyl-L-lysine;
[(E3-independent) ubiquitin-conjugating enzyme]-S-monoubiquitinyl-L-cysteine;
[(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine
Comment
The enzyme transfers a single ubiquitin directly from an ubiquitinated E1 ubiquitin-activating enzyme to itself, and on to a lysine residue of the acceptor protein without involvement of E3 ubiquitin transferases (cf. EC
2.3.2.26 , EC
2.3.2.27 ). It forms a labile ubiquitin adduct in the presence of E1, ubiquitin, and Mg2+-ATP and catalyses the conjugation of ubiquitin to protein substrates, independently of E3. This transfer has only been observed with small proteins. In vitro a transfer to small acceptors (e.g. L-lysine, N-acetyl-L-lysine methyl ester) has been observed [1].
History
EC 2.3.2.24 created 2015
Orthology
K10581 ubiquitin-conjugating enzyme E2 O
Genes
NGI : 103732691(Ube2o) 103735503 103749793
XLA : 108701078 108701130 108702822(ube2o.S)
MASI : 127446047 127448992
CHAR : 105900194 116219736
SASA : 106564911 106588250
STRU : 115178788 115208634
ARUT : 117423727 117424342
DME : Dmel_CG10254(CG10254)
DSI : Dsimw501_GD18366(Dsim_GD18366)
API : 100168139 100574674 100575202 103309139
PPOI : 119113546 119113547
HRO : HELRODRAFT_111131 HELRODRAFT_191359
SHX : MS3_00002946(UBE2O_1)
ATH : AT1G53023 AT1G53025 AT2G16920(UBC23) AT2G33770(PHO2) AT3G15355(UBC25)
ALY : 9315537 9320091 9321150 9327787 9330481
CRB : 17888898 17891486 17891818 17891928 17891941 17897148
CSAT : 104700065 104742802 104747486 104748205 104749488 104758637 104765364 104766994 104778233 104780079 104786183 104791685
EUS : EUTSA_v10011411mg EUTSA_v10016214mg EUTSA_v10020387mg EUTSA_v10022524mg
BRP : 103832701 103840805 103846023 103848904 103849943 103859619 103860493 103862491 103865294 103868465 103869928 103871164
BNA : 106345910 106349489 106360430 106376752 106379602 106401376 106402476 106402927 106409857 106418316 106423068 106428696 106431999 106439405 106439825 106447193 106448092 106449396 111205372 111207901 125586979 125588379 125598498
BOE : 106295540 106296706 106298567 106304622 106306280 106316808 106328062 106335259 106341814
RSZ : 108807642 108812090 108812092 108812293 108814400 108820709 108830343 108830975 108853821 108858914 108860077 108860083 108861432 108862701
THJ : 104807377 104810477 104815551 104820543
CPAP : 110812246 110818934 110821536
CIT : 102608976 102616731 102622894 102627024 102627978
CIC : CICLE_v10005250mg CICLE_v10007821mg CICLE_v10014194mg CICLE_v10014998mg CICLE_v100277353m
PVY : 116111342 116115770 116121429 116124396 116125262
MINC : 123193244 123196367 123196372 123219338 123223553 123223684 123227701
TCC : 18593984 18596849 18603667 18603668 18607524 18608712
GRA : 105761276 105772919 105774065 105776971 105779091 105779900 105794870 105800699
GHI : 107886776 107889390 107898556 107901724 107901725 107902660 107909291 107917794 107925175 107928783 107944364 107949109 121221052
GAB : 108456106 108460544 108470392 108475896 108482225 108483580 108484013 108489657
HSYR : 120123843 120148269 120150753 120159231 120171301 120175364 120210485 120213675
DZI : 111275049 111275836 111280310 111281786 111282250 111291116 111298307 111301679 111307178 111308737 111309289
EGR : 104417548 104417549 104418963 104441012 104448439 120288729 120294864
GMX : 100306246 100784814 100791144 100791343 100797350 100799332 100800819 100805463 100809513 100809740 100809853 100810318 100810772 100810844 100811389 100812552
GSJ : 114369635 114376343 114376506 114376611 114379645 114379687 114379980 114387601 114392938 114393479 114393481 114401190 114412760 114416876 114419543 114420734 114421563
PVU : PHAVU_002G183500g PHAVU_002G233800g PHAVU_003G115200g PHAVU_003G115300g PHAVU_003G115400g PHAVU_005G072300g PHAVU_006G185400g PHAVU_007G018800g PHAVU_011G157600g
VRA : 106755006 106766525 106766851 106767715 106769048 106772091 106775244 106777424
VAR : 108318758 108319705 108320700 108326000 108329974 108334208 108335074 108342933
VUN : 114170510 114175562 114175731 114177386 114177913 114179377 114185266 114187912 114189746 114191444
VUM : 124827558 124827559 124834664 124838828 124842470 124844189 124845875 124845893 124847191
CCAJ : 109790334 109796048 109803264 109804045 109805362 109807368 109807371 109818449
APRC : 113853984 113855294 113858479 113865278 113870948 113872326 113874695 113874696
MTR : 112417997 112418640 11408487 11411910 11443820 11446955 120575827 120577350 120577652 25485624 25487341 25487342 25491186 25493164 25498138 25498140 25499949
TPRA : 123885345 123892168 123895171 123895176 123895177 123895178 123895293 123896468 123896469 123908015 123914094 123916430 123918683
CAM : 101495261 101498894 101499482 101505903 101508783 101511457 101512988
PSAT : 127075413 127082177 127086339 127097944 127100485 127106254 127106416 127108174 127128444 127129407 127129409 127131866 127131867
VVO : 131602685 131602943 131607658 131607660 131620346 131627981 131627982 131628000 131628010 131637033 131637962 131644957 131647830 131647831 131647832 131649273
LJA : Lj0g3v0109419.1(Lj0g3v0109419.1) Lj0g3v0266399.1(Lj0g3v0266399.1) Lj0g3v0304639.1(Lj0g3v0304639.1) Lj0g3v0326929.1(Lj0g3v0326929.1) Lj0g3v0355129.1(Lj0g3v0355129.1) Lj1g3v1651360.1(Lj1g3v1651360.1) Lj2g3v3054240.1(Lj2g3v3054240.1) Lj4g3v0451220.1(Lj4g3v0451220.1) Lj4g3v0451220.2(Lj4g3v0451220.2) Lj4g3v2435000.1(Lj4g3v2435000.1) Lj4g3v2435000.2(Lj4g3v2435000.2) Lj6g3v2030860.1(Lj6g3v2030860.1)
ADU : 107462587 107462823 107463563 107467380 107468268 107480799 107482028
AIP : 107612864 107614779 107617817 107620132 107622175 107631228 107632063
AHF : 112703200 112703204 112707406 112712198 112714590 112723177 112723178 112733494 112734753 112769254 112777090 112783504 112791468 112792518
LANG : 109325777 109333031 109334319 109339345 109339470 109343501 109348150 109354510
PCIN : 129285718 129305811 129310479 129320871
QSA : O6P43_000962 O6P43_002140 O6P43_012647 O6P43_021871 O6P43_021872 O6P43_028815 O6P43_029101
FVE : 101294540 101301966 101302537 101303407 101304069 101308002 101309921 101310504
RCN : 112165635 112165636 112165637 112169724 112185410 112197628 112199288 112199444 112199499 112199500 112202765
PPER : 18766012 18768507 18779093 18780418 18792079
PMUM : 103323204 103324768 103326325 103328394 103334844
PAVI : 110751705 110753808 110766779 110767000 110773301
PDUL : 117616288 117621535 117624381 117624502 117636827
MDM : 103403530 103403633 103405663 103413292 103427325 103431500 103434792 103440797 103444912 103446408
MSYL : 126582058 126586599 126587194 126589460 126608141 126610973 126615673 126621937 126622347
PXB : 103928830 103931647 103934006 103944211 103946623 103947619 103948537 103948545 103965624
ZJU : 107417807 107418976 107422454 107424757 107427293
MNT : 21386484 21388276 21388277 21391730 21392605 21397647
CSAV : 115698240 115702909 115721899
CSV : 101204759 101219881 101220698 101221014
CMO : 103484521 103493488 103497343 103497784
BHJ : 120073078 120085878 120089276 120092860
MCHA : 111009807 111010440 111012245 111015425
CMAX : 111469743 111476837 111478555 111480464 111483941 111494283
CMOS : 111433133 111436820 111443352 111446374 111448445 111451318
CPEP : 111777628 111778700 111788588 111796101 111796600 111803832
RCU : 8258830 8258831 8262059 8273568 8279507 8283673
JCU : 105641806 105643558 105643683 105644271 105649542
HBR : 110635111 110641965 110644238 110648001 110650909 110669401
MESC : 110601332 110603196 110605117 110607209 110611441 110613037
POP : 18095585 18103023 18104628 7456794 7460818 7470409 7485896 7494240
PEU : 105111088 105115392 105122433 105128033 105129215 105134577 105138866 105140763 105141208
PALZ : 118031342 118038159 118038281 118040442 118046126 118047304 118050384 118058831 118062491
JRE : 108990632 108992732 108993378 108994701 108999571 109006609 109011512 109012985 109020617 109021203
CILL : 122276135 122278864 122281164 122290497 122300356 122304000 122309707 122313535 122317890 122317896
CAVE : 132166933 132170395 132175284 132181101 132181238 132181605
QSU : 111988776 111994491 111996029 112002150 112002161 112002163 112037929
QLO : 115949839 115949841 115950985 115955316 115957434 115967794 115978209 115989164
TWL : 119985010 119988720 119988809 119989587 119990058 119991287 120000055 120002104 120007631 120012806 120015202
VVI : 100241396 100258896 100262286 100264294 100266813
VRI : 117908929 117911243 117914087 117915167 117923906 117926723
SLY : 101243938 101244501 101250073 101261893 101267744
SPEN : 107005689 107007981 107011001 107024308 107032300
SOT : 102587645 102591333 102593834 102596954
SSTN : 125848062 125862777 125863886 125865786 125868358 125878013
SDUL : 129870854 129872106 129877418 129885305 129890810 129891538
CANN : 107840582 107842436 107845523 107849056 107849973 107859104 107878334
LBB : 132599340 132604489 132616651 132621261 132628806 132636477
NTA : 107775542 107789970 107792179 107801604 107808318 107811493 107812248 107812737 107817450 107818475 107821991 107832816
NSY : 104212422 104218135 104223553 104225299 104228499 104231458
NTO : 104087450 104097573 104099865 104116266 104117415 104118680
NAU : 109208746 109211665 109213921 109217559 109226650 109230736
INI : 109161406 109165106 109165341 109165351 109167339 109174172 109188316 109188375
ITR : 116010422 116011150 116020269 116021219 116029149 116032082
SIND : 105165385 105167905 105169800 105173050 105177004
OEU : 111370579 111371221 111377800 111378692 111380397 111382707 111386076 111386077 111387120 111394200 111400680
EGT : 105951468 105954645 105960666 105967683 105970413
SSPL : 121748259 121773260 121775948 121776493 121776804 121786169 121787336 121804545 121808131
SMIL : 131013318 131013393 131020905 131025438
APAN : 127250170 127254101 127254175 127260672 127262044
HAN : 110883874 110885722 110892531 110895730 110900202 110901432 110926300 110927681 110928845
ECAD : 122578391 122595247 122597536 122599550 122605091 122606718 122606735 122609483 122610445
LSV : 111880179 111882201 111882203 111899929 111910186 111917331
CCAV : 112500439 112502640 112516909 112519849 112522631 112522669 112525244
DCR : 108199099 108208976 108211809 108215580 108223211 108225921 108225934 108227122 108227617
CSIN : 114260253 114279675 114279702 114281913 114284071 114285058 114286259 114316527
RVL : 131312507 131313638 131323229 131323655 131325719 131328827 131330516
AEW : 130751667 130751936 130760806 130764182 130773714 130781277 130782284 130783814 130788931 130790036 130792274 130793574
BVG : 104888462 104891564 104891566 104900700 104902805 104903907
SOE : 110790046 110790249 110800181 110801468 110801478 110805497 110805498
CQI : 110683668 110685198 110693276 110693621 110697294 110704459 110706915 110718515 110720168 110739116
ATRI : 130798658 130805117 130805794 130815913
MOF : 131144872 131147997 131150560 131156039 131156628
NNU : 104599386 104599599 104604279 104605032 104608223
MING : 122061519 122071055 122071783 122071784 122073263 122083895
TSS : 122638914 122638917 122645744 122653172 122657963 122661690
PSOM : 113272906 113272911 113272913 113272922 113272923 113272924 113272925 113272926 113272984 113274124 113275111 113275779 113275896 113279073 113279356 113279529 113281618 113283251 113287631 113288986 113291232 113305069 113305086 113305097 113306868 113313089 113316117 113323712 113348823 113356289 113358707
OSA : 107276513 107277097 4324280 4324290 4326008 4338168 4339579 4346643
DOSA : Os01t0125900-01(Os01g0125900) Os01t0232500-01(Os01g0232500) Os01t0233900-01(Os01g0233900) Os01t0677500-00(Os01g0677500) Os05t0232500-01(Os05g0232500) Os09t0294300-01(Os09g0294300)
OBR : 102699621 102700744 102702149 102706137 102706382 102715856
OGL : 127756037 127773374 127773524 127774729 127777584 127778112 127785237
BDI : 100822941 100825985 100829288 100829591 100839385 100841219 100841950 100845785
ATS : 109733100 109739038 109739120 109746297 109748786 109749680 109751578 109751580 109753429 109760591 109760592 109768423 109773380 109776213 109776215 109776715 109780160 109786599 109787010
TDC : 119266821 119266991 119267698 119267705 119267706 119268828 119274438 119275567 119275573 119276781 119280531 119280532 119280535 119288715 119291308 119291317 119292847 119299684 119309743 119309744 119310062 119329782 119333353 119349478 119349481 119351932 119359790 119360339 119360340 119368128 119368897
TAES : 123040219 123040221 123042243 123042739 123042742 123042744 123042789 123046573 123048379 123049735 123049932 123049933 123050677 123057586 123057595 123059976 123060811 123060818 123060819 123061893 123064393 123064395 123064399 123067599 123068317 123069374 123069379 123069380 123070584 123073632 123073635 123076869 123077886 123077891 123077892 123078989 123081556 123090230 123097069 123116462 123116844 123120666 123120667 123145120 123145168 123176094 123180754 123183024 123183027 123185834 123186001 123186611
TUA : 125526441 125528604 125529575 125533835 125533988 125542455 125543385 125543387 125543436 125543438 125543439 125544567 125544568 125546478 125546479 125547627 125547631 125547632 125547635 125553958 125554879 125554880
LPER : 127296822 127303844 127304206 127309780 127316017 127327518 127329548 127329702 127329708 127337148 127340995 127342051 127342058 127342060 127342061 127343328 127346762
LRD : 124646552 124648427 124648494 124648500 124649366 124649396 124657294 124660404 124662835 124663345 124663839 124665371 124665837 124666359 124666990 124669982 124670930 124671079 124671080 124671081 124671082 124671100 124671102 124671103 124671104 124671105 124671178 124681890 124690898 124690930 124697738 124697740 124708343
SBI : 110433916 8066641 8070404 8074577 8077561 8081621
ZMA : 100194284 100304369 100502171 100502472 103651829
SITA : 101757739 101757978 101760919 101761493 101767028 101767608 101781999 101782409 101783215 101784433
SVS : 117848022 117848024 117849024 117849291 117849293 117850019 117856450 117857905 117858464 117858949
PVIR : 120663903 120664675 120666508 120666509 120673969 120675244 120676830 120676973 120688719 120697060 120697873 120701100 120701101 120705481 120705487 120706139
PHAI : 112884183 112885895 112893198 112893473 112893579
PDA : 103709500 103715581 103718297 103718674
EGU : 105042389 105042400 105045840 105047412 105048331
MUS : 103992791 103996401 103996557 103997076 103998771
ZOF : 121970851 121979227 121981234 121981235 121983879 121985114 121985115 122029255 122032939 122033530 122040748 122043257 122044341 122045279 122047778 122048064
DCT : 110101685 110103090 110112472
PEQ : 110023616 110026624 110032240 110035866
AOF : 109822604 109822620 109842102 109848528 109850883
MSIN : 131237169 131237171 131245523
NCOL : 116246678 116247878
ATR : 18424546 18437080 18448926
SMO : SELMODRAFT_20278 SELMODRAFT_30432
MPP : MICPUCDRAFT_48363(JGI:MicpuC2_48363)
PNO : SNOG_05701(SNOG_05700)
CCAC : CcaHIS019_0500860(CcaverHIS019_0500860)
HIR : HETIRDRAFT_166454(ubc3)
ABP : AGABI1DRAFT117056(AGABI1DRAFT_117056)
ABV : AGABI2DRAFT114397(AGABI2DRAFT_114397)
SCM : SCHCO_01142917(SCHCODRAFT_01142917)
» show all
Taxonomy
Reference
Authors
Pickart CM, Rose IA
Title
Functional heterogeneity of ubiquitin carrier proteins.
Journal
J Biol Chem 260:1573-81 (1985)
Reference
Authors
Hoeller D, Hecker CM, Wagner S, Rogov V, Dotsch V, Dikic I
Title
E3-independent monoubiquitination of ubiquitin-binding proteins.
Journal
Reference
Authors
Ramanathan HN, Zhang G, Ye Y
Title
Monoubiquitination of EEA1 regulates endosome fusion and trafficking.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 2.3.2.24
ExPASy - ENZYME nomenclature database: 2.3.2.24