KEGG ENZYME: 2.3.2.35

ENZYME: 2.3.2.35

Entry

EC 2.3.2.35                 Enzyme

Name

capsaicin synthase;
CS (gene name) (ambiguous);
Pun1 (locus name)

Class

Transferases;
Acyltransferases;
Aminoacyltransferases

Sysname

(6E)-8-methylnon-6-enoyl-CoA:vanillylamine 8-methylnon-6-enoyltransferase

Reaction(IUBMB)

(6E)-8-methylnon-6-enoyl-CoA + vanillylamine = CoA + capsaicin [RN:R12626]

Reaction(KEGG)

R12626

Substrate

(6E)-8-methylnon-6-enoyl-CoA [CPD:C22289];
vanillylamine [CPD:C16666]

Product

CoA [CPD:C00010];
capsaicin [CPD:C06866]

Comment

The enzyme, found only in plants that belong to the Capsicum genus, catalyses the last step in the biosynthesis of capsaicinoids. The enzyme catalyses the acylation of vanillylamine by a branched-chain fatty acid. The exact structure of the fatty acid determines the type of capsaicinoid formed.

History

EC 2.3.2.35 created 2020

Pathway

ec00996

Biosynthesis of various alkaloids

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K24277

capsaicin synthase

Genes

SLY:	101255851 101256149

SPEN:

107010618

SOT:

102597608

SSTN:

125848852 125864209 125866859

SDUL:

129878763

CANN:

107859694

LBB:	132605491 132608352 132642122

ITR:	116026080 116026905

Reference

1 [PMID:12175073]

Authors

Blum E, Liu K, Mazourek M, Yoo EY, Jahn M, Paran I

Title

Molecular mapping of the C locus for presence of pungency in Capsicum.

Journal

Genome 45:702-5 (2002)
DOI:10.1139/g02-031

Reference

2 [PMID:15918882]

Authors

Stewart C Jr, Kang BC, Liu K, Mazourek M, Moore SL, Yoo EY, Kim BD, Paran I, Jahn MM

Title

The Pun1 gene for pungency in pepper encodes a putative acyltransferase.

Journal

Plant J 42:675-88 (2005)
DOI:10.1111/j.1365-313X.2005.02410.x

Sequence

[cann:107859694]

Reference

3 [PMID:24441736]

Authors

Kim S, Park M, Yeom SI, Kim YM, Lee JM, Lee HA, Seo E, Choi J, Cheong K, Kim KT, Jung K, Lee GW, Oh SK, Bae C, Kim SB, Lee HY, Kim SY, Kim MS, Kang BC, Jo YD, Yang HB, Jeong HJ, Kang WH, Kwon JK, Shin C, Lim JY, Park JH, Huh JH, Kim JS, Kim BD, Cohen O, Paran I, Suh MC, Lee SB, Kim YK, Shin Y, Noh SJ, Park J, Seo YS, Kwon SY, Kim HA, Park JM, Kim HJ, Choi SB, Bosland PW, Reeves G, Jo SH, Lee BW, Cho HT, Choi HS, Lee MS, Yu Y, Do Choi Y, Park BS, van Deynze A, Ashrafi H, Hill T, Kim WT, Pai HS, Ahn HK, Yeam I, Giovannoni JJ, Rose JK, Sorensen I, Lee SJ, Kim RW, Choi IY, Choi BS, Lim JS, Lee YH, Choi D

Title

Genome sequence of the hot pepper provides insights into the evolution of pungency in Capsicum species.

Journal

Nat Genet 46:270-8 (2014)
DOI:10.1038/ng.2877

Other DBs

ExplorEnz - The Enzyme Database:

2.3.2.35

IUBMB Enzyme Nomenclature:

2.3.2.35

ExPASy - ENZYME nomenclature database:

2.3.2.35

BRENDA, the Enzyme Database:

2.3.2.35

DBGET integrated database retrieval system