Entry Name crocetin glucosyltransferase;
Class Transferases;BRITE hierarchy
Sysname UDP-alpha-D-glucose:crocetin 8-O-D-glucosyltransferase
Reaction(IUBMB) (1) UDP-alpha-D-glucose + crocetin = UDP + beta-D-glucosyl crocetin [RN:
R09757 ];
(2) UDP-alpha-D-glucose + beta-D-glucosyl crocetin = UDP + bis(beta-D-glucosyl) crocetin [RN:
R09758 ];
(3) UDP-alpha-D-glucose + beta-D-gentiobiosyl crocetin = UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin [RN:
R09759 ]
Reaction(KEGG) Substrate UDP-alpha-D-glucose [CPD:
C00029 ];
crocetin [CPD:
C08588 ];
beta-D-glucosyl crocetin [CPD:
C19867 ];
beta-D-gentiobiosyl crocetin [CPD:
C19869 ]
Product UDP [CPD:
C00015 ];
beta-D-glucosyl crocetin [CPD:
C19867 ];
bis(beta-D-glucosyl) crocetin [CPD:
C19868 ];
beta-D-gentiobiosyl beta-D-glucosyl crocetin [CPD:
C17513 ]
Comment In the plants Crocus sativus and Gardenia jasminoides this enzyme esterifies a free carboxyl group of crocetin and some crocetin glycosyl esters. The enzyme from Gardenia can also form glucosyl esters with 4-coumarate, caffeate and ferulate [3].
History EC 2.4.1.271 created 2011
Pathway ec00999 Biosynthesis of various plant secondary metabolites
ec01110 Biosynthesis of secondary metabolites
Orthology K21371 crocetin glucosyltransferase
Reference 1
Authors Cote, F., Cormier, F., Dufresne, C. and Willemot, C.
Title Properties of a glucosyltransferase involved in crocin synthesis.
Journal Plant Sci 153:55-63 (2000)
Reference Authors Moraga AR, Nohales PF, Perez JA, Gomez-Gomez L
Title Glucosylation of the saffron apocarotenoid crocetin by a glucosyltransferase isolated from Crocus sativus stigmas.
Journal Sequence Reference Authors Nagatoshi M, Terasaka K, Owaki M, Sota M, Inukai T, Nagatsu A, Mizukami H
Title UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin in Gardenia jasminoides.
Journal Sequence Other DBs ExPASy - ENZYME nomenclature database: 2.4.1.271
