KEGG ENZYME: 2.4.1.278

ENZYME: 2.4.1.278

Entry

EC 2.4.1.278                Enzyme

Name

3-alpha-mycarosylerythronolide B desosaminyl transferase;
EryCIII;
dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose:3-alpha-mycarosylerythronolide B 3-dimethylamino-4,6-dideoxy-alpha-D-glucosyltransferase

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

dTDP-3-dimethylamino-3,4,6-trideoxy-alpha-D-glucopyranose:3-alpha-mycarosylerythronolide B 3-dimethylamino-3,4,6-trideoxy-beta-D-glucosyltransferase

Reaction(IUBMB)

dTDP-D-desosamine + 3-alpha-L-mycarosylerythronolide B = dTDP + erythromycin D [RN:R05531]

Reaction(KEGG)

R05531

Substrate

dTDP-D-desosamine [CPD:C11911];
3-alpha-L-mycarosylerythronolide B [CPD:C06630]

Product

dTDP [CPD:C00363];
erythromycin D [CPD:C06633]

Comment

The enzyme is involved in erythromycin biosynthesis.

History

EC 2.4.1.278 created 2012, modified 2014

Pathway

ec00522

Biosynthesis of 12-, 14- and 16-membered macrolides

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K14368

3-alpha-mycarosylerythronolide B desosaminyl transferase

Genes

SANL:

KZO11_24460

SCYE:

R2B67_24645 R2B67_24660

SNR:	SNOUR_10095

SGF:	HEP81_07908

SCIR:

STRCI_006233

SLON:

LGI35_31705

SDRZ:

NEH16_32015

SKG:	KJK29_16100

AER:	AERYTH_15900

NEX:	NE857_19565

SEN:	SACE_0726(eryCIII)

AEY:	CDG81_13160

» show all

Reference

1 [PMID:16218575]

Authors

Yuan Y, Chung HS, Leimkuhler C, Walsh CT, Kahne D, Walker S

Title

In vitro reconstitution of EryCIII activity for the preparation of unnatural macrolides.

Journal

J Am Chem Soc 127:14128-9 (2005)
DOI:10.1021/ja053704n

Sequence

[sen:SACE_0726]

Reference

2 [PMID:15303858]

Authors

Lee HY, Chung HS, Hang C, Khosla C, Walsh CT, Kahne D, Walker S

Title

Reconstitution and characterization of a new desosaminyl transferase, EryCIII, from the erythromycin biosynthetic pathway.

Journal

J Am Chem Soc 126:9924-5 (2004)
DOI:10.1021/ja048836f

Sequence

[sen:SACE_0726]

Reference

3 [PMID:22056329]

Authors

Moncrieffe MC, Fernandez MJ, Spiteller D, Matsumura H, Gay NJ, Luisi BF, Leadlay PF

Title

Structure of the glycosyltransferase EryCIII in complex with its activating P450 homologue EryCII.

Journal

J Mol Biol 415:92-101 (2012)
DOI:10.1016/j.jmb.2011.10.036

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.278

IUBMB Enzyme Nomenclature:

2.4.1.278

ExPASy - ENZYME nomenclature database:

2.4.1.278

BRENDA, the Enzyme Database:

2.4.1.278

DBGET integrated database retrieval system