KEGG ENZYME: 2.4.1.301

ENZYME: 2.4.1.301

Entry

EC 2.4.1.301                Enzyme

Name

2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase;
kanE (gene name);
kanM2 (gene name)

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-alpha-D-kanosamine:2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase

Reaction(IUBMB)

(1) UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyneamine = UDP + kanamycin A [RN:R10424];
(2) UDP-alpha-D-kanosamine + neamine = UDP + kanamycin B [RN:R10425];
(3) UDP-alpha-D-kanosamine + paromamine = UDP + kanamycin C [RN:R10426];
(4) UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyparomamine = UDP + kanamycin X [RN:R10427]

Reaction(KEGG)

R10424 R10425 R10426 R10427;
(other) R11271 R11272

Substrate

UDP-alpha-D-kanosamine [CPD:C12211];
2'-deamino-2'-hydroxyneamine [CPD:C20350];
neamine [CPD:C01441];
paromamine [CPD:C01743];
2'-deamino-2'-hydroxyparomamine [CPD:C20353]

Product

UDP [CPD:C00015];
kanamycin A [CPD:C01822];
kanamycin B [CPD:C00825];
kanamycin C [CPD:C01823];
kanamycin X [CPD:C20614]

Comment

Involved in the biosynthetic pathway of kanamycins. The enzyme characterized from the bacterium Streptomyces kanamyceticus can also accept UDP-alpha-D-glucose with lower efficiency [2].

History

EC 2.4.1.301 created 2013

Pathway

ec00524

Neomycin, kanamycin and gentamicin biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K20573

2'-deamino-2'-hydroxyneamine 1-alpha-D-kanosaminyltransferase

Reference

1 [PMID:19911031]