KEGG ENZYME: 2.4.1.303

ENZYME: 2.4.1.303

Entry

EC 2.4.1.303                Enzyme

Name

UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,3-galactosyltransferase;
WbbD;
WbbD beta3Gal-transferase;
UDP-Gal:GlcNAc-R beta1,3-galactosyltransferase;
UDP-Gal:GlcNAcalpha-pyrophosphate-R beta1,3-galactosyltransferase;
UDP-Gal:GlcNAc-R galactosyltransferase

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-alpha-D-galactose:N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol 3-beta-galactosyltransferase (configuration-inverting)

Reaction(IUBMB)

UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + beta-D-Gal-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol [RN:R10432]

Reaction(KEGG)

R10432

Substrate

UDP-alpha-D-galactose [CPD:C00052];
N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol [CPD:C01289]

Product

UDP [CPD:C00015];
beta-D-Gal-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol [CPD:C20617]

Comment

The enzyme is involved in the the biosynthesis of the O-antigen repeating unit of Escherichia coli O7:K1 (VW187). Requires Mn2+. cf. EC 2.4.1.343, UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol alpha-1,3-galactosyltransferase.

History

EC 2.4.1.303 created 2013, modified 2017

Orthology

K18788

UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,3-galactosyltransferase

Genes

ECT:	ECIAI39_0985(wbbD)

EOC:	CE10_2348(wbbD)

EBR:	ECB_01936(wbbD)

ECOB:

C3029_14320

EBL:	ECD_01936(wbbD)

EBE:	B21_01923(ybl89)

SHQ:	A0259_13985

Reference

1 [PMID:15625181]

Authors

Riley JG, Menggad M, Montoya-Peleaz PJ, Szarek WA, Marolda CL, Valvano MA, Schutzbach JS, Brockhausen I

Title

The wbbD gene of E. coli strain VW187 (O7:K1) encodes a UDP-Gal: GlcNAc{alpha}-pyrophosphate-R {beta}1,3-galactosyltransferase involved in the biosynthesis of O7-specific lipopolysaccharide.

Journal

Glycobiology 15:605-13 (2005)
DOI:10.1093/glycob/cwi038

Sequence

[ag:AAC27537]

Reference

2 [PMID:18536883]

Authors

Brockhausen I, Riley JG, Joynt M, Yang X, Szarek WA

Title

Acceptor substrate specificity of UDP-Gal: GlcNAc-R beta1,3-galactosyltransferase (WbbD) from Escherichia coli O7:K1.

Journal

Glycoconj J 25:663-73 (2008)
DOI:10.1007/s10719-008-9127-7