KEGG ENZYME: 2.4.1.304

ENZYME: 2.4.1.304

Entry

EC 2.4.1.304                Enzyme

Name

UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,4-galactosyltransferase;
WfeD;
UDP-Gal:GlcNAc-R 1,4-Gal-transferase;
UDP-Gal:GlcNAc-pyrophosphate-lipid beta-1,4-galactosyltransferase

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-alpha-D-galactose:N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol beta-1,4-galactosyltransferase

Reaction(IUBMB)

UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol [RN:R10433]

Reaction(KEGG)

R10433

Substrate

UDP-alpha-D-galactose [CPD:C00052];
N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol [CPD:C01289]

Product

UDP [CPD:C00015];
beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol [CPD:C20618]

Comment

The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of the bacterium Shigella boydii B14. The activity is stimulated by Mn2+ or to a lesser extent by Mg2+, Ca2+, Ni2+ or Pb2+.

History

EC 2.4.1.304 created 2013

Orthology

K21364

UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,4-galactosyltransferase

Genes

ELN:	NRG857_10355

ELF:

LF82_350

VRO:	BSZ04_22135

VMT:	QYQ96_12575

PPR:

PBPRA2677

PSPI:

PS2015_1756

SHM:	Shewmr7_1395

SEUR:

FM038_015500

PPIS:

B1L02_14610

PTU:	PTUN_a0641

PSEN:

PNC201_02750(wfeD)

PAGA:

PAGA_a0486

PXI:	J5O05_07210

PVB:	J5X90_03360

PSAZ:

PA25_03780

PFLI:

CTT31_02685

PMAZ:

R5H13_02700

» show all

Reference

1 [PMID:21057010]

Authors

Xu C, Liu B, Hu B, Han Y, Feng L, Allingham JS, Szarek WA, Wang L, Brockhausen I

Title

Biochemical characterization of UDP-Gal:GlcNAc-pyrophosphate-lipid beta-1,4-Galactosyltransferase WfeD, a new enzyme from Shigella boydii type 14 that catalyzes the second step in O-antigen repeating-unit synthesis.

Journal

J Bacteriol 193:449-59 (2011)
DOI:10.1128/JB.00737-10

Sequence

[ag:ACD37055]

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.304

IUBMB Enzyme Nomenclature:

2.4.1.304

ExPASy - ENZYME nomenclature database:

2.4.1.304

BRENDA, the Enzyme Database:

2.4.1.304

DBGET integrated database retrieval system