KEGG   ENZYME: 2.4.1.332
Entry
EC 2.4.1.332                Enzyme                                 
Name
1,2-alpha-glucosylglycerol phosphorylase;
2-O-alpha-D-glucopyranosylglycerol phosphorylase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
2-O-alpha-D-glucopyranosyl-glycerol:phosphate beta-D-glucosyltransferase
Reaction(IUBMB)
2-O-alpha-D-glucopyranosyl-glycerol + phosphate = beta-D-glucose 1-phosphate + glycerol [RN:R11127]
Reaction(KEGG)
R11127
Substrate
2-O-alpha-D-glucopyranosyl-glycerol [CPD:C11546];
phosphate [CPD:C00009]
Product
beta-D-glucose 1-phosphate [CPD:C00663];
glycerol [CPD:C00116]
Comment
The enzyme has been isolated from the bacterium Bacillus selenitireducens. In the absence of glycerol the enzyme produces alpha-D-glucopyranose and phosphate from beta-D-glucopyranose 1-phosphate. In this reaction the glucosyl residue is transferred to a water molecule with an inversion of the anomeric conformation.
History
EC 2.4.1.332 created 2014
Orthology
K21355  1,2-alpha-glucosylglycerol phosphorylase
Genes
BBEV: BBEV_2395(kojP)
BSE: Bsel_2816
HOR: Hore_20830
HAS: Halsa_1740
HHL: Halha_1547
SSM: Spirs_0890
SFC: Spiaf_1195
Reference
1  [PMID:24466148]
  Authors
Nihira T, Saito Y, Ohtsubo K, Nakai H, Kitaoka M
  Title
2-O-alpha-D-glucosylglycerol phosphorylase from Bacillus selenitireducens MLS10 possessing hydrolytic activity on beta-D-glucose 1-phosphate.
  Journal
PLoS One 9:e86548 (2014)
DOI:10.1371/journal.pone.0086548
  Sequence
[bse:Bsel_2816]
Reference
2  [PMID:24828502]
  Authors
Touhara KK, Nihira T, Kitaoka M, Nakai H, Fushinobu S
  Title
Structural basis for reversible phosphorolysis and hydrolysis reactions of 2-O-alpha-glucosylglycerol phosphorylase.
  Journal
J Biol Chem 289:18067-75 (2014)
DOI:10.1074/jbc.M114.573212
  Sequence
[bse:Bsel_2816]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.332
IUBMB Enzyme Nomenclature: 2.4.1.332
ExPASy - ENZYME nomenclature database: 2.4.1.332
BRENDA, the Enzyme Database: 2.4.1.332

  All links  
Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (8)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (7)   
Protein sequence (3)   
   UniProt (1)   
   SWISS-PROT (1)   
   RefSeq(pep) (1)   
DNA sequence (1)   
   RefSeq(nuc) (1)   
Protein domain (8)   
   InterPro (8)   
All databases (26)   

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