The enzyme, isolated from the bacterium Clostridium phytofermentans, catalyses a reversible reaction. Substrates for the phosphorolytic reaction are alpha-1,3-linked oligoglucans with a polymerisation degree of 3 or more. Nigerose (i.e. 3-O-alpha-D-glucopyranosyl-D-glucopyranose) is not phosphorylyzed but can serve as substrate in the reverse direction (cf. EC 2.4.1.279, nigerose phosphorylase).
History
EC 2.4.1.334 created 2014
Reference
1
Authors
Nihira, T., Nishimoto, M., Nakai, H., Ohtsubo, K., and Kitaoka, M.
Title
Characterization of two phosphorylases for alpha-1,3-oligoglucans from Clostridium phytofermentans.