KEGG   ENZYME: 2.4.1.341
Entry
EC 2.4.1.341                Enzyme                                 
Name
alpha-1,2-colitosyltransferase;
wbgN (gene name)
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
GDP-beta-L-colitose:beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine L-colitosyltransferase (configuration-inverting)
Reaction(IUBMB)
GDP-beta-L-colitose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine = GDP + alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
Substrate
GDP-beta-L-colitose [CPD:C20613];
beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine [CPD:C06372]
Product
GDP [CPD:C00035];
alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
Comment
The enzyme, characterized from the bacterium Escherichia coli O55:H7, participates in the biosynthesis of an O-antigen. The reaction involves anomeric inversion, and does not require any metal ions. The enzyme is highly specific towards the acceptor, exclusively recognizing lacto-N-biose, but can accept GDP-L-fucose as the donor with almost the same activity as with GDP-beta-L-colitose.
History
EC 2.4.1.341 created 2016
Orthology
K20883  O55-antigen biosynthesis alpha-1,2-colitosyltransferase
Genes
EOKG2583_2558(wbgN)
ELRECO55CA74_12305
Reference
1  [PMID:26703456]
  Authors
Wu Z, Zhao G, Li T, Qu J, Guan W, Wang J, Ma C, Li X, Zhao W, Wang PG, Li L
  Title
Biochemical characterization of an alpha1,2-colitosyltransferase from Escherichia coli O55:H7.
  Journal
Glycobiology 26:493-500 (2016)
DOI:10.1093/glycob/cwv169
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.341
IUBMB Enzyme Nomenclature: 2.4.1.341
ExPASy - ENZYME nomenclature database: 2.4.1.341
BRENDA, the Enzyme Database: 2.4.1.341

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