KEGG   ENZYME: 2.4.1.344
Entry
EC 2.4.1.344                Enzyme                                 
Name
type 2 galactoside alpha-(1,2)-fucosyltransferase;
blood group H alpha-2-fucosyltransferase (ambiguous);
guanosine diphosphofucose-galactoside 2-L-fucosyltransferase (ambiguous);
alpha-(1->2)-L-fucosyltransferase (ambiguous);
alpha-2-fucosyltransferase (ambiguous);
alpha-2-L-fucosyltransferase (ambiguous);
blood-group substance H-dependent fucosyltransferase (ambiguous);
guanosine diphosphofucose-glycoprotein 2-alpha-fucosyltransferase (ambiguous);
guanosine diphosphofucose-lactose fucosyltransferase;
GDP fucose-lactose fucosyltransferase;
guanosine diphospho-L-fucose-lactose fucosyltransferase;
guanosine diphosphofucose-beta-D-galactosyl-alpha-2-L-fucosyltransferase (ambiguous);
guanosine diphosphofucose-galactosylacetylglucosaminylgalactosylglucosylceramide alpha-L-fucosyltransferase (ambiguous);
guanosine diphosphofucose-glycoprotein 2-alpha-L-fucosyltransferase (ambiguous);
H-gene-encoded beta-galactoside alpha(1->2)fucosyltransferase;
beta-galactoside alpha(1->2)fucosyltransferase (ambiguous);
GDP-L-fucose:lactose fucosyltransferase;
GDP-beta-L-fucose:beta-D-galactosyl-R 2-alpha-L-fucosyltransferase (ambiguous);
FUT1 (gene name);
FUT2 (gene name)
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
GDP-beta-L-fucose:beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R alpha-(1,2)-L-fucosyltransferase (configuration-inverting)
Reaction(IUBMB)
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R [RN:R11601]
Reaction(KEGG)
R11601
Substrate
GDP-beta-L-fucose [CPD:C00325];
beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R [CPD:C00694]
Product
GDP [CPD:C00035];
alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R [CPD:C21494]
Comment
The enzyme acts on a glycoconjugates where R (see reaction) is a glycoprotein or glycosphingolipid. The recognized moiety of the substrate is known as a type 2 histo-blood group antigen precursor disaccharide, and the action of the enzyme produces an H type 2 antigen. Humans possess two enzymes able to catalyse this reaction, encoded by the FUT1 and FUT2 genes (also known as the H and Secretor genes, respectively), but only FUT1 is expressed in red blood cells. cf. EC 2.4.1.69, type 1 galactoside alpha-(1,2)-fucosyltransferase.
History
EC 2.4.1.344 created 2017
Reference
1  [PMID:804484]
  Authors
Basu S, Basu M, Chien JL.
  Title
Enzymatic synthesis of a blood group H-related glycosphingolipid by an alpha-fucosyltransferase from bovine spleen.
  Journal
J Biol Chem 250:2956-62 (1975)
Reference
2
  Authors
Grollman, A.P.
  Title
GDP-L-fucose:lactose fucosyltransferase from mammary gland.
  Journal
Methods Enzymol 8:351-353 (1966)
Reference
3  [PMID:2464598]
  Authors
Ernst LK, Rajan VP, Larsen RD, Ruff MM, Lowe JB
  Title
Stable expression of blood group H determinants and GDP-L-fucose: beta-D-galactoside 2-alpha-L-fucosyltransferase in mouse cells after transfection with human DNA.
  Journal
J Biol Chem 264:3436-47 (1989)
Reference
4  [PMID:2118655]
  Authors
Larsen RD, Ernst LK, Nair RP, Lowe JB
  Title
Molecular cloning, sequence, and expression of a human GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase cDNA that can form the H blood group antigen.
  Journal
Proc Natl Acad Sci U S A 87:6674-8 (1990)
DOI:10.1073/pnas.87.17.6674
  Sequence
[hsa:2523]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.344
IUBMB Enzyme Nomenclature: 2.4.1.344
ExPASy - ENZYME nomenclature database: 2.4.1.344
BRENDA, the Enzyme Database: 2.4.1.344

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Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (3)   
   KEGG REACTION (1)   
   KEGG RCLASS (2)   
Protein sequence (915)   
   UniProt (56)   
   SWISS-PROT (31)   
   RefSeq(pep) (828)   
DNA sequence (2895)   
   RefSeq(nuc) (1539)   
   GenBank (678)   
   EMBL (678)   
Protein domain (1)   
   InterPro (1)   
All databases (3818)   

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