KEGG   ENZYME: 2.4.1.360
Entry
EC 2.4.1.360                Enzyme                                 
Name
2-hydroxyflavanone C-glucosyltransferase;
OsCGT
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-alpha-D-glucose:2'-hydroxy-beta-oxodihydrochalcone C6/8-beta-D-glucosyltransferase
Reaction(IUBMB)
UDP-alpha-D-glucose + a 2'-hydroxy-beta-oxodihydrochalcone = UDP + a 3'-(beta-D-glucopyranosyl)-2'-hydroxy-beta-oxodihydrochalcone [RN:R12290]
Reaction(KEGG)
R12290
Substrate
UDP-alpha-D-glucose [CPD:C00029];
2'-hydroxy-beta-oxodihydrochalcone [CPD:C22066]
Product
UDP [CPD:C00015];
3'-(beta-D-glucopyranosyl)-2'-hydroxy-beta-oxodihydrochalcone [CPD:C22067]
Comment
The enzyme has been characterized in Oryza sativa (rice), various Citrus spp., Glycine max (soybean), and Fagopyrum esculentum (buckwheat). Flavanone substrates require a 2-hydroxy group. The meta-stable flavanone substrates such as 2-hydroxynaringenin exist in an equilibrium with open forms such as 1-(4-hydroxyphenyl)-3-(2,4,6-trihydroxyphenyl)propane-1,3-dione, which are the actual substrates for the glucosyl-transfer reaction (see EC 1.14.14.162, flavanone 2-hydroxylase). The enzyme can also act on dihydrochalcones. The enzymes from citrus plants can catalyse a second C-glycosylation reaction at position 5.
History
EC 2.4.1.360 created 2018
Orthology
K23154  2-hydroxyflavanone C-glucosyltransferase
Genes
CIT: 102618156
CIC: CICLE_v10013670mg CICLE_v10031419mg
PVY: 116127954 116141746 116145333
MINC: 123206911 123215088 123215092 123217198 123224883
TCC: 18601595 18610301 18610303 18610304
GRA: 105780808
GHI: 107893931 107897380 107920122 121216078
GAB: 108459103 108462068
HSYR: 120118169 120130956 120130965 120163099 120170923
DZI: 111288214 111292515
EGR: 104423661 104442902 104445237 104445238 104447177 104447179 104447180 104447181
GMX: 100776116(UGT708D1) 100816555
GSJ: 114368684 114390418
PVU: PHAVU_004G137900g PHAVU_004G1381000 PHAVU_004G138200g PHAVU_004G1384000
VRA: 106757078 106772373
VAR: 108344625 108344626 108344628
VUN: 114182225 114182226 114182227
VUM: 124822228 124822236 124827477 124827481
CCAJ: 109792376 109792446 109792447 109792448
APRC: 113860191 113867818 113867999 113868048 113869104 113869105
MTR: 11427859 11446167 11446168
TPRA: 123917202
CAM: 101494394(UGT708B1)
PSAT: 127106869 127119626
VVO: 131607323 131643210 131646531
LJA: Lj2g3v0604430.1(Lj2g3v0604430.1)
ADU: 107489286 127747436
AIP: 107641855 107643597
AHF: 112750303 112750304 112801561 112801564
LANG: 109330820 109340164 109340165 109341800 109356596
PCIN: 129292221 129293210 129297654 129301274 129311624 129311828
QSA: O6P43_025520 O6P43_025521 O6P43_025522 O6P43_032940 O6P43_032941 O6P43_032942
FVE: 101310443 105352450
RCN: 112185174 112186643 112199715
PPER: 18782310 18782830 18783263 18792255
PMUM: 103321673 103330063 103330064 103330065
PAVI: 110751271 110755571 110774182 110774187
MDM: 103410171 103416637 103417071
MSYL: 126607801 126610675 126612604
PXB: 103951017 103951080
ZJU: 107415922 107415924 107428877
MNT: 21403834 21403836 21403839 21403843
CSAV: 115699562 115714258
CSV: 101222215
CMO: 103489166
BHJ: 120080748
MCHA: 111019065
CMAX: 111482455
CMOS: 111458784
CPEP: 111777626
RCU: 8281269
JCU: 105630954 105644118 105644120
HBR: 110666182 110666183
MESC: 110609574 110623334
POP: 7472619 7484554
PEU: 105111096 105133493
PALZ: 118049548 118055117
JRE: 109003115
CILL: 122312731 122313778
CAVE: 132179797
QSU: 112009093 112012259 112012284 112022402
QLO: 115975566 115978863 115978864
TWL: 119991268 119992245
SLY: 101265797 101267953
SPEN: 107009690 107009692
SOT: 102596262 102597670 102601756
SSTN: 125847867
SDUL: 129872764 129873925
CANN: 107860487 107860489 107860490
LBB: 132621173 132621174
NTA: 107778051 107781900
NSY: 104241134
NTO: 104092631
NAU: 109240202
INI: 109180632
ITR: 116012027
SIND: 105177295 105177356 105177357
OEU: 111400904
EGT: 105971021 105974614 105974623
SSPL: 121754543 121757663
SMIL: 130989635 130989637
SHIS: 125218448 125221929
APAN: 127258277 127258278
ECAD: 122578608
CCAV: 112513046
DCR: 108196233 108226125
CSIN: 114256993 114269560 114296545 114308090 114308973
RVL: 131302281 131302765 131335121
AEW: 130768832 130768833 130769483 130772934 130772977 130787106 130789290
BVG: 104886914 104887295
SOE: 110796490
CQI: 110735058
MOF: 131155232 131160654 131160662
NNU: 104598527 104603347
MING: 122063843 122091300
TSS: 122672102 122672103
PSOM: 113346508
OSA: 4340787 4340788 9266253 9270077
DOSA: Os06t0288300-01(Os06g0288300) Os06t0289200-01(Os06g0289200) Os06t0289900-01(Os06g0289900)
OBR: 102712546 102712824 102713645 102720054
OGL: 127775505 127775891 127776000 127777222
BDI: 100825799 100826193
ATS: 109732191 109732196 109764127 109764128 109782690
TDC: 119284006 119327435 119328551 119332597 119336082 119336186 119340053 119341887 119348704 119348705
TAES: 123060189 123094271 123094282 123094294 123137106 123150761 123153518 123154251 123158939 123159486 123159778 123165825 123166487 123166980 123182377
TUA: 125520341 125529343 125536389
LPER: 127317200 127317202 127317454
LRD: 124646926 124647675 124674747 124674803 124675432 124675979
SBI: 8066139 8073750 8076408 8076410
ZMA: 100191917 100194125 100217220 100281608 100282556
SITA: 101781599 101782011
SVS: 117851226 117851227 117851257
PVIR: 120643951 120684770 120703361
PHAI: 112888580 112889866 112889867
PDA: 103708718 103716468 120111946
EGU: 105049993 105050648 105060740
MUS: 103974397
ZOF: 121989572 121989575 121989576 121998507 121998512 121998513 122037171 122037172
DCT: 110094296 110097095 110103872 110116333
PEQ: 110031195 110031196 110031197 110037294
AOF: 109849013
MSIN: 131219195
NCOL: 116258506 116258585 116258828
ATR: 18438457
 » show all
Reference
1  [PMID:19411659]
  Authors
Brazier-Hicks M, Evans KM, Gershater MC, Puschmann H, Steel PG, Edwards R
  Title
The C-glycosylation of flavonoids in cereals.
  Journal
J Biol Chem 284:17926-34 (2009)
DOI:10.1074/jbc.M109.009258
  Sequence
[osa:9266253]
Reference
2  [PMID:25142187]
  Authors
Nagatomo Y, Usui S, Ito T, Kato A, Shimosaka M, Taguchi G
  Title
Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon.
  Journal
Plant J 80:437-48 (2014)
DOI:10.1111/tpj.12645
  Sequence
[ag:BAP90360 BAP90361]
Reference
3  [PMID:25979175]
  Authors
Hirade Y, Kotoku N, Terasaka K, Saijo-Hamano Y, Fukumoto A, Mizukami H
  Title
Identification and functional analysis of 2-hydroxyflavanone C-glucosyltransferase in soybean (Glycine max).
  Journal
FEBS Lett 589:1778-86 (2015)
DOI:10.1016/j.febslet.2015.05.010
  Sequence
[gmx:100776116]
Reference
4  [PMID:28370711]
  Authors
Ito T, Fujimoto S, Suito F, Shimosaka M, Taguchi G
  Title
C-Glycosyltransferases catalyzing the formation of di-C-glucosyl flavonoids in citrus plants.
  Journal
Plant J 91:187-198 (2017)
DOI:10.1111/tpj.13555
  Sequence
[cit:102618156]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.360
IUBMB Enzyme Nomenclature: 2.4.1.360
ExPASy - ENZYME nomenclature database: 2.4.1.360
BRENDA, the Enzyme Database: 2.4.1.360

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Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (1)   
   KEGG REACTION (1)   
Gene (352)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (351)   
Protein sequence (15)   
   UniProt (7)   
   SWISS-PROT (7)   
   RefSeq(pep) (1)   
DNA sequence (3)   
   RefSeq(nuc) (1)   
   GenBank (1)   
   EMBL (1)   
Protein domain (3)   
   InterPro (3)   
All databases (378)   

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