KEGG ENZYME: 2.4.1.376

ENZYME: 2.4.1.376

Entry

EC 2.4.1.376                Enzyme

Name

EGF-domain serine glucosyltransferase;
POGLUT1 (gene name) (ambiguous);
rumi (gene name) (ambiguous)

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-alpha-D-glucose:[protein with EGF-like domain]-L-serine O-beta-glucosyltransferase (configuration-inverting)

Reaction(IUBMB)

UDP-alpha-D-glucose + [protein with EGF-like domain]-L-serine = UDP + [protein with EGF-like domain]-3-O-(beta-D-glucosyl)-L-serine [RN:R09315]

Reaction(KEGG)

R09315(G)

Substrate

UDP-alpha-D-glucose [CPD:C00029];
[protein with EGF-like domain]-L-serine

Product

UDP [CPD:C00015];
[protein with EGF-like domain]-3-O-(beta-D-glucosyl)-L-serine

Comment

The enzyme, found in animals and insects, is involved in the biosynthesis of the alpha-D-xylosyl-(1->3)-alpha-D-xylosyl-(1->3)-beta-D-glucosyl trisaccharide on epidermal growth factor-like (EGF-like) domains. Glycosylation takes place at the serine in the C-X-S-X-P-C motif. The enzyme is bifunctional also being active with UDP-alpha-xylose as donor (EC 2.4.2.63, EGF-domain serine xylosyltransferase). When present on Notch proteins, the trisaccharide functions as a modulator of the signalling activity of this protein.

History

EC 2.4.1.376 created 2020

Pathway

ec00514

Other types of O-glycan biosynthesis

Orthology

K13667

EGF-domain serine glucosyl/xylosyltransferase

Genes

HSA:	56983(POGLUT1)

PTR:	460605(POGLUT1)

PPS:	100996033(POGLUT1)

GGO:	101148987(POGLUT1)

PON:	100442484(POGLUT1)

NLE:	100601138(POGLUT1)

HMH:	116466911(POGLUT1)

MCC:	712357(POGLUT1)

MCF:	102140463(POGLUT1)

MTHB:

126947747

MNI:	105470341(POGLUT1)

CSAB:

103228230(POGLUT1)

CATY:

105589726(POGLUT1)

PANU:

101020502(POGLUT1)

TGE:	112619466(POGLUT1)

MLEU:

105544623(POGLUT1)

RRO:	104657614(POGLUT1)

RBB:	108531821(POGLUT1)

TFN:	117083471(POGLUT1)

PTEH:

111543775(POGLUT1)

CANG:

105510726(POGLUT1)

CJC:	100392996(POGLUT1)

SBQ:	101028421(POGLUT1)

CIMI:

108296446(POGLUT1)

CSYR:

103254568(POGLUT1)

MMUR:

105879924(POGLUT1)

LCAT:

123630515(POGLUT1)

PCOQ:

105808319(POGLUT1)

OGA:	100964166(POGLUT1)

MMU:	224143(Poglut1)

MCAL:

110311390(Poglut1)

MPAH:

110329469(Poglut1)

RNO:	288091(Poglut1)

MCOC:

116085903(Poglut1)

ANU:	117718090(Poglut1)

MUN:	110555395(Poglut1)

CGE:	100760325(Poglut1)

MAUA:

101841266(Poglut1)

PROB:

127227163(Poglut1)

PLEU:

114701491(Poglut1)

MORG:

121461999(Poglut1)

MFOT:

126505845

AAMP:

119825319(Poglut1)

NGI:	103741939(Poglut1)

HGL:	101707033(Poglut1)

CPOC:

100736113(Poglut1)

CCAN:

109694532(Poglut1)

DORD:

105987916(Poglut1)

DSP:	122098547(Poglut1)

PLOP:

125351729(Poglut1)

NCAR:

124992920

MMMA:

107136291(Poglut1)

OCU:

100344082

OPI:	101534837(POGLUT1)

TUP:	102478881(POGLUT1)

GVR:	103598139(POGLUT1)

CFA:	487993(POGLUT1)

CLUD:

112677830(POGLUT1)

VVP:	112913459(POGLUT1)

VLG:	121471241(POGLUT1)

NPO:	129503622(POGLUT1)

AML:	100480912(POGLUT1)

UMR:	103678843(POGLUT1)

UAH:	113251857(POGLUT1)

UAR:	123784033(POGLUT1)

ELK:

111147444

LLV:

125097742

MPUF:

101674307(POGLUT1)

MNP:	132012204(POGLUT1)

MLK:	131825087(POGLUT1)

NVS:	122908531(POGLUT1)

ORO:	101386979(POGLUT1)

EJU:	114214817(POGLUT1)

ZCA:	113918644(POGLUT1)

MLX:	118007071(POGLUT1)

NSU:	110582449(POGLUT1)

LWW:	102742937(POGLUT1)

FCA:	101088101(POGLUT1)

PYU:	121036275(POGLUT1)

PCOO:

112849871(POGLUT1)

PBG:	122491471(POGLUT1)

PVIV:

125175426(POGLUT1)

LRUF:

124526775

PTG:	102972728(POGLUT1)

PPAD:

109266432(POGLUT1)

PUC:

125921780

AJU:

106968287

HHV:	120225967(POGLUT1)

BTA:	511862(POGLUT1)

BOM:	102277916(POGLUT1)

BIU:	109576903(POGLUT1)

BBUB:

102406475(POGLUT1)

BBIS:

104992473(POGLUT1)

CHX:	102191273(POGLUT1)

OAS:	101105708(POGLUT1)

BTAX:

128053862(POGLUT1)

ODA:	120871250(POGLUT1)

CCAD:

122445579(POGLUT1)

MBEZ:

129560291(POGLUT1)

SSC:	100525611(POGLUT1)

CFR:	102523608(POGLUT1)

CBAI:

105081589(POGLUT1)

CDK:	105090329(POGLUT1)

VPC:	102537660(POGLUT1)

BACU:

103003776(POGLUT1)

BMUS:

118894693(POGLUT1)

LVE:	103085383(POGLUT1)

OOR:	101279837(POGLUT1)

DLE:	111167187(POGLUT1)

PCAD:

102976502(POGLUT1)

PSIU:

116753361(POGLUT1)

NASI:

112413462(POGLUT1)

ECB:	100071030(POGLUT1)

EPZ:	103547415(POGLUT1)

EAI:	106823701(POGLUT1)

MYB:	102262926(POGLUT1)

MYD:	102752908(POGLUT1)

MMYO:

118676595(POGLUT1)

MLF:	102425666(POGLUT1)

PKL:	118724329(POGLUT1)

EFUS:

103290710(POGLUT1)

MNA:	107527335(POGLUT1)

DRO:	112320557(POGLUT1)

SHON:

118986584(POGLUT1)

AJM:	119059192(POGLUT1)

PDIC:

114490862(POGLUT1)

PHAS:

123827490(POGLUT1)

MMF:	118617745(POGLUT1)

PPAM:

129069649(POGLUT1) 129082265

HAI:	109388471(POGLUT1)

RFQ:	117013247(POGLUT1)

PALE:

102880714(POGLUT1)

PGIG:

120586655(POGLUT1)

PVP:	105302432(POGLUT1)

RAY:	107508364(POGLUT1)

MJV:	108392243(POGLUT1)

TOD:	119257496(POGLUT1)

SARA:

101537818(POGLUT1)

SETR:

126026211(POGLUT1)

LAV:	100661725(POGLUT1)

TMU:

101351318

ETF:	101663934(POGLUT1)

DNM:	101426603(POGLUT1)

MDO:	100016192(POGLUT1)

GAS:	123239479(POGLUT1)

SHR:	100918363(POGLUT1)

AFZ:

127554103

PCW:	110208064(POGLUT1)

OAA:	103167158(POGLUT1)

GGA:	418455(POGLUT1)

PCOC:

116242023(POGLUT1)

MGP:	100541132(POGLUT1)

CJO:	107322048(POGLUT1)

TPAI:

128090969(POGLUT1)

LMUT:

125684857(POGLUT1)

NMEL:

110401460(POGLUT1)

APLA:

101800875(POGLUT1)

ACYG:

106039966(POGLUT1)

CATA:

118245015(POGLUT1)

AFUL:

116499011(POGLUT1)

TGU:	100217574(POGLUT1)

LSR:	110476510(POGLUT1)

SCAN:

103818416(POGLUT1)

PMOA:

120504041(POGLUT1)

OTC:	121344734(POGLUT1)

PRUF:

121360388(POGLUT1)

GFR:	102041454(POGLUT1)

FAB:	101814002(POGLUT1)

OMA:	130256574(POGLUT1)

PHI:	102100422(POGLUT1)

PMAJ:

107212428(POGLUT1)

CCAE:

111943579(POGLUT1)

CCW:	104689442(POGLUT1)

CBRC:

103613727(POGLUT1)

ETL:	114059604(POGLUT1)

ZAB:	102069592(POGLUT1)

ACHL:

103798995(POGLUT1)

SVG:	106857013(POGLUT1)

MMEA:

130588924(POGLUT1)

HRT:	120748895(POGLUT1)

FPG:	101919362(POGLUT1)

FCH:	102049145(POGLUT1)

CLV:	102092788(POGLUT1)

EGZ:	104123016(POGLUT1)

NNI:	104015008(POGLUT1)

PCRI:

104037160(POGLUT1)

PLET:

104614461(POGLUT1)

EHS:	104504040(POGLUT1)

PCAO:

104039437(POGLUT1)

ACUN:

113475980(POGLUT1)

TALA:

104358498(POGLUT1)

PADL:

103916889(POGLUT1)

AFOR:

103895007(POGLUT1)

ACHC:

115343537(POGLUT1)

HALD:

104317415(POGLUT1)

HLE:	104831819(POGLUT1)

AGEN:

126049023

GCL:

127029472

CCRI:

104154964(POGLUT1)

CSTI:

104555340(POGLUT1)

CMAC:

104473426(POGLUT1)

MUI:	104533748(POGLUT1)

BREG:

104629544(POGLUT1)

FGA:	104071060(POGLUT1)

GSTE:

104256770(POGLUT1)

LDI:	104352334(POGLUT1)

MNB:	103771390(POGLUT1)

OHA:	104337367(POGLUT1)

NNT:	104399575(POGLUT1)

SHAB:

115601512(POGLUT1)

DPUB:

104299528(POGLUT1)

PGUU:

104458130(POGLUT1)

ACAR:

104526605(POGLUT1)

CPEA:

104395775(POGLUT1)

CVF:	104290928(POGLUT1)

RTD:	128902032(POGLUT1)

CUCA:

104056026(POGLUT1)

TEO:	104370222(POGLUT1)

BRHI:

104488053(POGLUT1)

AAM:	106490712(POGLUT1)

AROW:

112974465(POGLUT1)

NPD:	112948253(POGLUT1)

TGT:	104577454(POGLUT1)

DNE:	112979297(POGLUT1)

SCAM:

104149203(POGLUT1)

ASN:	102370297(POGLUT1)

AMJ:	102567059(POGLUT1)

CPOO:

109324962(POGLUT1)

GGN:	109302342(POGLUT1)

PSS:	102453336(POGLUT1)

CMY:	102931530(POGLUT1)

CCAY:

125644773(POGLUT1)

DCC:	119841301(POGLUT1)

CPIC:

101932750(POGLUT1)

TST:	117888540(POGLUT1)

CABI:

116839124(POGLUT1)

MRV:	120404839(POGLUT1)

ACS:	100562217(poglut1)

ASAO:

132771952(POGLUT1)

PVT:	110088504(POGLUT1)

SUND:

121927149(POGLUT1)

PBI:	103059086(POGLUT1)

PMUR:

107289023(POGLUT1)

CTIG:

120303890(POGLUT1)

TSR:	106557375(POGLUT1)

PGUT:

117663818(POGLUT1)

APRI:

131200281(POGLUT1)

PTEX:

113436333(POGLUT1)

NSS:	113409744(POGLUT1)

VKO:	123029452(POGLUT1)

PMUA:

114595966(POGLUT1)

PRAF:

128412898(POGLUT1)

ZVI:	118085544(POGLUT1)

HCG:	128352495(POGLUT1)

GJA:	107117554(POGLUT1)

STOW:

125431847(POGLUT1)

EMC:	129325944(POGLUT1)

XLA:	108707382(poglut1.L) 446695(poglut1.S)

XTR:	493476(poglut1)

NPR:	108803675(POGLUT1)

RTEM:

120921634(POGLUT1)

BBUF:

120994300(POGLUT1)

BGAR:

122922824(POGLUT1)

MUO:	115470601(POGLUT1)

GSH:	117359209(POGLUT1)

DRE:	641495(poglut1)

SRX:	107739820 107742654(poglut1)

SANH:

107700102(poglut1)

SGH:	107552365 107552368 107588837(poglut1)

CCAR:

109083311 109093877

CAUA:

113044357

CGIB:

127949653

PTET:

122349514(poglut1)

LROH:

127170415(poglut1)

OMC:	131546047(poglut1)

PPRM:

120495527

RKG:

130087802

MAMB:

125272295(poglut1)

CIDE:

127514270

MASI:

127443718

TROS:

130555615(poglut1)

TDW:	130434204(poglut1)

MANU:

129444203(poglut1)

IPU:

108259071

IFU:	128602156(poglut1)

PHYP:

113537643(poglut1)

SMEO:

124377779(poglut1)

TFD:	113648659(poglut1)

TVC:	132839659(poglut1)

AMEX:

103033884(poglut1)

CMAO:

118798258(poglut1)

EEE:

113580326

CHAR:

105893299(poglut1)

TRU:	101073599(poglut1)

TFS:	130522169(poglut1)

TNG:	GSTEN00019585G001

LCO:	104937494(poglut1)

NCC:	104961748(poglut1)

TBEN:

117498987(poglut1)

CGOB:

115019011(poglut1)

PGEO:

117458282(poglut1)

GACU:

117546680(poglut1)

EMAC:

134860621(poglut1)

ELY:	117249764(poglut1)

EFO:	125885110(poglut1)

PLEP:

121962835(poglut1)

SLUC:

116044591(poglut1)

ECRA:

117939288(poglut1)

ESP:	116673834(poglut1)

PFLV:

114550792(poglut1)

GAT:	120820785(poglut1)

PPUG:

119222474(poglut1)

AFB:	129111879(poglut1)

CLUM:

117743228(poglut1)

PSWI:

130191512(poglut1)

MSAM:

119901475(poglut1)

SCHU:

122872262(poglut1)

CUD:	121506500(poglut1)

ALAT:

119015434(poglut1)

MZE:	101485624(poglut1)

ONL:	100701894 109196814

OAU:	116321027(poglut1)

OLA:	101164781(poglut1)

OML:	112160080(poglut1)

CSAI:

133425022(poglut1)

XMA:	102235605(poglut1)

XCO:	114140207(poglut1)

XHE:	116715917(poglut1)

PRET:

103476691(poglut1)

PFOR:

103156859(poglut1)

PLAI:

106935503(poglut1)

PMEI:

106930358(poglut1)

GAF:	122827302(poglut1)

PPRL:

129353787 129379065

CVG:	107086895(poglut1)

CTUL:

119790353(poglut1)

GMU:	124861651(poglut1)

NFU:	107381039(poglut1)

KMR:	108245685(poglut1)

ALIM:

106519172(poglut1)

NWH:	119428681(poglut1)

AOCE:

111566550(poglut1)

MCEP:

125000800(poglut1)

CSEM:

103389764(poglut1)

POV:	109646568(poglut1)

SSEN:

122783841(poglut1)

HHIP:

117774754(poglut1)

HSP:	118103255(poglut1)

SMAU:

118287040(poglut1)

LCF:

108900589

SDU:	111222664(poglut1)

SLAL:

111645992(poglut1)

XGL:	120797979(poglut1)

HCQ:	109510428(poglut1)

SSCV:

125988145

SBIA:

133509889(poglut1)

PEE:	133410533(poglut1)

PTAO:

133484061(poglut1)

BPEC:

110153299(poglut1)

MALB:

109955765(poglut1)

BSPL:

114848730(poglut1)

SJO:	128354190(poglut1)

SASA:

106574542(poglut1)

STRU:

115196125(poglut1)

OTW:	112266428(poglut1)

OMY:	110495774(poglut1)

OGO:	124010602(poglut1)

ONE:	115129773(poglut1)

OKI:	109879428(poglut1)

OKE:	118363831(poglut1) 118370267

SALP:

111953666(poglut1) 111971149

SNH:	120050498(poglut1)

CCLU:

121559251(poglut1)

ELS:	105019877(poglut1)

SFM:	108937696(poglut1)

PKI:	111841189(poglut1)

AANG:

118222368(poglut1)

LOC:	102682938(poglut1)

PSPA:

121320912 121327858

ARUT:

117406369 117406601

PSEX:

120522966(poglut1)

LCM:	102351249(POGLUT1)

CMK:	103190449(poglut1)

RTP:	109911835(poglut1)

CPLA:

122560311(poglut1)

HOC:	132823018(poglut1)

LERI:

129707348(poglut1)

PMRN:

116952240(POGLUT1)

LRJ:	133355521(POGLUT1) 133355555

BFO:

118415884

BBEL:

109470814

CIN:

100184131

SCLV:

120330062

SPU:

577218

LPIC:

129256537 129256538

APLC:

110988715

AJC:

117103889

DME:	Dmel_CG31139(CG31139) Dmel_CG31152(rumi)

DER:

6554883

DSE:	6607494 6607495

DSI:	Dsimw501_GD18424(Dsim_GD18424) Dsimw501_GD18425(Dsim_GD18425)

DYA:	Dyak_GE24006

DAN:

6501062

DSR:	110177291 110177292

DPO:	4800928 6896966

DPE:	6587777 6587843

DMN:	108156655 108156657

DWI:	6646867 6646868

DGR:

6567946

DMO:	Dmoj_GI10779

DAZ:

108620940

DNV:

108659291

DHE:

111599985

CCAT:

101456689

BOD:

106616254

BDR:

105231804

RZE:	108365540 108365541 108370983

AOQ:

129241103

TDA:	119679427 119680127

MDE:	101888953 131806781

SCAC:

106095710

LCQ:

111679679

LSQ:

119599582

GFS:

119641955

ECOE:

129938667

CLON:

129609748

HIS:

119660306

AGA:

4576887

ACOZ:

120948660

AARA:

120897451

AMER:

121588482

ASTE:

118508364

AFUN:

125766557

AMOU:

128309356

AALI:

118458947

AAG:

5574413

AALB:

109419219

CQU:	CpipJ_CPIJ013394

CPII:

120426826

CNS:

116339754

BCOO:

119077890

ACER:

107993295

ALAB:

122710882

ADR:

102671739

AFLR:

100869271

BIM:

100741194

BBIF:

117212917

BVK:

117233701

BVAN:

117158981

BTER:

100647386

BAFF:

126926539

BPYO:

122571304

BPAS:

132908853

FVI:

122534046

CCAL:

108626989

OBB:

114880443

OLG:

117608431

MGEN:

117217408

NMEA:

116426311

CGIG:

122401010

SOC:

105198301

MPHA:

105835701

AEC:

105151122

ACEP:

105619866

PBAR:

105424385

VEM:

105566276

HST:

105190365

DQU:

106744914

CFO:

105255412

FEX:

115241862

LHU:

105675701

PGC:

109858215

OBO:

105286123

PCF:

106788604

PFUC:

122522007

VPS:

122636567

VCRB:

124432305

VVE:

124956500

CSOL:

105364958

LHT:

122502888

LBD:

127282602

MDL:

103577459

CGLO:

123263457

FAS:

105271343

DAM:

107036734

AGIF:

122860918

CINS:

118074356

VCAN:

122417945

CCIN:

107271816

DSM:

124404462

NPT:

124210990

NFB:

124174693

NLO:

107223947

NVG:

124296926

AROA:

105683296

TCA:

657023

DPA:

109534702

SOY:

115889520

AGRG:

126740472

CSET:

123311794

AGB:

108910951

LDC:

111515105

NVL:

108563144

APLN:

108741150

PPYR:

116171266

OTU:

111424958

BMOR:

101746748

BMAN:

114247988

MSEX:

115451419

DPL:	KGM_204317

BANY:

112051421

MJU:

123864316

NIQ:

126778771

VCD:

124537024

MCIX:

123664389

PMAC:

106712461

PPOT:

106111052

PXU:

106117029

PRAP:

111004054

PBX:

123717886

PNAP:

125061904

ZCE:

119829988

CCRC:

123701833

LSIN:

126971278

AAGE:

121729681

HAW:

110370875

HZE:

124636764

TNL:	113492711 113507129

SLIU:

111353427

OFU:

114360538

PXY:

105388550

PGW:

126381800

CFEL:

113370866 113370868 113371601

CCRN:

123306131

API:

100161314

DNX:

107167867

AGS:

114121796

RMD:

113560337

ACOO:

126839315

DVT:

126903171

BTAB:

109038780

DCI:	108254559 113473964

CLEC:

106671962

HHAL:

106688258

NLU:

111053727

HVI:

124369239

MQU:

128997977

PHU:	Phum_PHUM609790

ZNE:

110830225

CSEC:

111873166

SGRE:

126299250

BROR:

134541870

IEL:

124170836

FCD:

110851414

DPX:	DAPPUDRAFT_305151

DMK:	116920852 123466486

DPZ:

124337049

PVM:

113830472

PJA:

122265579

PCHN:

125043027

PMOO:

119585388 119585392

PCLA:

123768255

CQD:

128684761

PTRU:

123516009

ESN:

127003743

MNZ:

135215353

HAZT:

108673384

EAF:

111696558

LSM:

121114612

TCF:

131892669

PPOI:

119095389 119111021

DSV:

119450186

RSAN:

119383389

RMP:

119170362

VDE:

111243422

VJA:

111260713

TUT:

107372260

DPTE:

113798026

DFR:

124494145

CSCU:

111623380

PTEP:

107452840

ABRU:

129974940

UDV:

129219395

SDM:

118179673

LPOL:

106473417

PMEO:

129594268

TSP:

Tsp_12313

HRO:	HELRODRAFT_103469

LGI:	LOTGIDRAFT_225910

PVUL:

126828806

PCAN:

112572636

BGT:

106054990

GAE:

121368096

HRF:

124146556

HRJ:

124280208

CRG:	105327429 117686636

CVN:	111105932 111106423

CANU:

128158885

OED:

125657098

MYI:

110449580

PMAX:

117330662

MCAF:

127722829

MMER:

123529349

RPHI:

132724668

DPOL:

127880915

OBI:

106870958

OSN:

115212369

LAK:	106160124 106164609

NVE:	116616778 5506266

EPA:

110245281

ATEN:

116296167

AMIL:

114969502

PDAM:

113664486

SPIS:

111333788

DGT:

114521987

XEN:

124434882

HMG:

100206611

HSY:

130625013

TAD:	TRIADDRAFT_18924

AQU:

100638708

CRE:	CHLRE_07g344500v5 CHLRE_08g367750v5

VCN:	VOLCADRAFT_98596

APRO:

F751_5515

CCP:	CHC_T00008884001

PNO:	SNOG_12132

PTE:

PTT_09542

BZE:	COCCADRAFT_3545

BSC:	COCSADRAFT_187637

BOR:	COCMIDRAFT_110047

AALT:

CC77DRAFT_1028215

SMIN:

v1.2.002892.t1(symbB.v1.2.002892.t1) v1.2.004773.t1(symbB.v1.2.004773.t1)

EHX:	EMIHUDRAFT_227650 EMIHUDRAFT_242134 EMIHUDRAFT_249850 EMIHUDRAFT_252748 EMIHUDRAFT_441372 EMIHUDRAFT_79465

» show all

Reference

1 [PMID:28775322]

Authors

Li Z, Fischer M, Satkunarajah M, Zhou D, Withers SG, Rini JM

Title

Structural basis of Notch O-glucosylation and O-xylosylation by mammalian protein-O-glucosyltransferase 1 (POGLUT1).

Journal

Nat Commun 8:185 (2017)
DOI:10.1038/s41467-017-00255-7

Sequence

[hsa:56983]

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.376

IUBMB Enzyme Nomenclature:

2.4.1.376

ExPASy - ENZYME nomenclature database:

2.4.1.376

BRENDA, the Enzyme Database:

2.4.1.376

DBGET integrated database retrieval system