KEGG ENZYME: 2.4.1.384

ENZYME: 2.4.1.384

Entry

EC 2.4.1.384                Enzyme

Name

NDP-glycosyltransferase;
yjiC (gene name)

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

NDP-glycose:acceptor glycosyltransferase

Reaction(IUBMB)

an NDP-glycose + an acceptor = a glycosylated acceptor + NDP

Substrate

NDP-glycose;
acceptor [CPD:C00028]

Product

glycosylated acceptor;
NDP [CPD:C00454]

Comment

The enzyme, characterized from the bacterium Bacillus licheniformis DSM-13, is an extremely promiscuous glycosyltransferase. It can accept ADP-, GDP-, CDP-, TDP-, or UDP-activated glycose molecules as donors, and can glycosylate a large number of substrates, catalysing O-, N-, or S-glycosylation. While D-glucose is the primarily reported sugar being transferred, the enzyme has been shown to transfer D-galactose, 2-deoxy-D-glucose, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, L-fucose, L-rhamnose, D-glucuronate, and D-viosamine.

History

EC 2.4.1.384 created 2021

Orthology

K25230

NDP-glycosyltransferase

Genes

BSU:	BSU12220(yjiC)

BSR:

I33_1374

BSL:

A7A1_0231

BSH:	BSU6051_12220(yjiC)

BSY:	I653_06230

BSUT:

BSUB_01330(yjiC)

BSUL:

BSUA_01330(yjiC)

BSUS:

Q433_06955

BSO:	BSNT_07692(yjiC)

BSN:	BSn5_18070

BSQ:	B657_12220(yjiC)

BSX:	C663_1253(yjiC)

BSP:	U712_06330

BSS:	BSUW23_06215(yjiC)

BST:

GYO_1529

BIT:	BIS30_16840

BLI:	BL00446(yjiC)

BLD:	BLi01948(yjiC)

BLH:	BaLi_c19830(yjiC)

BAY:	RBAM_012300

BAQ:	BACAU_1181(yjiC)

BYA:	BANAU_1162(yjiC)

BAMP:

B938_06275

BQY:	MUS_1310(yjiC)

BAML:

BAM5036_1136(yjiC)

BAMA:

RBAU_1185(yjiC)

BAMN:

BASU_1164(yjiC)

BAMB:

BAPNAU_2561(yjiC)

BAMT:

AJ82_06895

BAMY:

V529_11730

BMP:	NG74_01252(oleD_2)

BAO:	BAMF_1309(yjiC)

BAZ:	BAMTA208_11035(yjiC)

BQL:	LL3_01317(yjiC)

BXH:	BAXH7_02259(yjiC)

BAMI:

KSO_013425

BAMC:

U471_12310

BAMF:

U722_06380

BSIA:

CWD84_15195

BAE:	BATR1942_03760

BVM:	B9C48_06160

BSON:

S101395_02951

BHT:	DIC78_03385

BMOJ:

HC660_12840(ppuG)

BTEQ:

G4P54_06460

BIQ:	AN935_06335

BSTR:

QI003_06585(ppuG)

BGY:

BGLY_2157

BCAB:

EFK13_06835

BRY:	M0696_06680

BJS:

MY9_1334

BACP:

SB24_03660

BACB:

OY17_09250

BACY:

QF06_05090

BACL:

BS34A_13530(yjiC)

BALM:

BsLM_1284

BACS:

AUL54_04470

BGI:	BGM20_00385

PSAB:

PSAB_16875

PDU:	PDUR_18615

PSOP:

KP014_23270

CPAS:

Clopa_1820

AACX:

DEACI_0885

SCHF:

IPT68_29230

AOG:	LH407_04080

AMOG:

QRX60_11940

ASIC:

Q0Z83_092980

» show all

Reference

1 [PMID:23974133]

Authors

Pandey RP, Parajuli P, Koirala N, Park JW, Sohng JK.

Title

Probing 3-hydroxyflavone for in vitro glycorandomization of flavonols by YjiC.

Journal

Appl Environ Microbiol 79:6833-8 (2013)
DOI:10.1128/AEM.02057-13

Reference

2 [PMID:24893262]

Authors

Pandey RP, Gurung RB, Parajuli P, Koirala N, Tuoi le T, Sohng JK.

Title

Assessing acceptor substrate promiscuity of YjiC-mediated glycosylation toward flavonoids.

Journal

Carbohydr Res 393:26-31 (2014)
DOI:10.1016/j.carres.2014.03.011

Sequence

[bld:BLi01948]

Reference

3 [PMID:25239890]

Authors

Pandey RP, Parajuli P, Shin JY, Lee J, Lee S, Hong YS, Park YI, Kim JS, Sohng JK.

Title

Enzymatic Biosynthesis of Novel Resveratrol Glucoside and Glycoside Derivatives.

Journal

Appl Environ Microbiol 80:7235-43 (2014)
DOI:10.1128/AEM.02076-14

Sequence

[bld:BLi01948]

Reference

4 [PMID:24949266]

Authors

Parajuli P, Pandey RP, Koirala N, Yoon YJ, Kim BG, Sohng JK.

Title

Enzymatic synthesis of epothilone A glycosides.

Journal

AMB Express 4:31 (2014)
DOI:10.1186/s13568-014-0031-1

Sequence

[bld:BLi01948]

Reference

5 [PMID:27444326]

Authors

Pandey RP, Parajuli P, Gurung RB, Sohng JK.

Title

Donor specificity of YjiC glycosyltransferase determines the conjugation of cytosolic NDP-sugar in in vivo glycosylation reactions.

Journal

Enzyme Microb Technol 91:26-33 (2016)
DOI:10.1016/j.enzmictec.2016.05.006

Reference

6 [PMID:32238768]

Authors

Bashyal P, Thapa SB, Kim TS, Pandey RP, Sohng JK.

Title

Exploring the Nucleophilic N- and S-Glycosylation Capacity of Bacillus licheniformis YjiC Enzyme.

Journal

J Microbiol Biotechnol 30:1092-1096 (2020)
DOI:10.4014/jmb.2001.01024

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.384

IUBMB Enzyme Nomenclature:

2.4.1.384

ExPASy - ENZYME nomenclature database:

2.4.1.384

BRENDA, the Enzyme Database:

2.4.1.384

All links

Gene (67)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (66)   
Protein sequence (4)   
   UniProt (2)   
   SWISS-PROT (2)   
Protein domain (4)   
   InterPro (4)   
All databases (75)   

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