KEGG   ENZYME: 2.4.1.387
Entry
EC 2.4.1.387                Enzyme                                 
Name
isomaltosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
alpha-isomaltosyl-(1->3)-1,4-alpha-D-glucan:1,4-alpha-D-glucan 3-alpha-isomaltosyltransferase
Reaction(IUBMB)
(1) 2 alpha-isomaltosyl-(1->4)-maltotriose = alpha-isomaltosyl-(1->3)-alpha-isomaltosyl-(1->4)-maltotriose + maltotriose [RN:R12984];
(2) alpha-isomaltosyl-(1->3)-alpha-isomaltosyl-(1->4)-maltotriose = cyclobis-(1->6)-alpha-nigerosyl + maltotriose [RN:R12985]
Reaction(KEGG)
R12984 R12985
Substrate
alpha-isomaltosyl-(1->4)-maltotriose [CPD:C22497];
alpha-isomaltosyl-(1->3)-alpha-isomaltosyl-(1->4)-maltotriose [CPD:C22498]
Product
alpha-isomaltosyl-(1->3)-alpha-isomaltosyl-(1->4)-maltotriose [CPD:C22498];
maltotriose [CPD:C01835];
cyclobis-(1->6)-alpha-nigerosyl
Comment
The enzyme, found in bacteria that produce cyclobis-(1->6)-alpha-nigerosyl, acts on the products of EC 2.4.1.24, 1,4-alpha-glucan 6-alpha;-glucosyltransferase. It catalyses the alpha-(1->3) transfer of the isomaltosyl moiety of one substrate to another, resulting in alpha-isomaltosyl-(1->3)-alpha-isomaltosyl-alpha-(1->4)-glucan formation. In addition, the enzyme catalyses the intramolecular cyclization of the product, eventually generating cyclobis-(1->6)-alpha-nigerosyl.
History
EC 2.4.1.387 created 2022
Orthology
K25763  isomaltosyltransferase
Reference
1  [PMID:12092816]
  Authors
Aga H, Maruta K, Yamamoto T, Kubota M, Fukuda S, Kurimoto M, Tsujisaka Y.
  Title
Cloning and sequencing of the genes encoding cyclic tetrasaccharide-synthesizing enzymes from Bacillus globisporus C11.
  Journal
Biosci Biotechnol Biochem 66:1057-68 (2002)
DOI:10.1271/bbb.66.1057
  Sequence
[ag:BAB88403]
Reference
2  [PMID:12400677]
  Authors
Nishimoto T, Aga H, Mukai K, Hashimoto T, Watanabe H, Kubota M, Fukuda S, Kurimoto M, Tsujisaka Y.
  Title
Purification and characterization of glucosyltransferase and glucanotransferase involved in the production of cyclic tetrasaccharide in Bacillus globisporus C11.
  Journal
Biosci Biotechnol Biochem 66:1806-18 (2002)
DOI:10.1271/bbb.66.1806
Reference
3  [PMID:14553982]
  Authors
Kim YK, Kitaoka M, Hayashi K, Kim CH, Cote GL.
  Title
A synergistic reaction mechanism of a cycloalternan-forming enzyme and a D-glucosyltransferase for the production of cycloalternan in Bacillus sp. NRRL B-21195.
  Journal
Carbohydr Res 338:2213-20 (2003)
DOI:10.1016/s0008-6215(03)00375-6
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.387
IUBMB Enzyme Nomenclature: 2.4.1.387
ExPASy - ENZYME nomenclature database: 2.4.1.387
BRENDA, the Enzyme Database: 2.4.1.387

  All links  
Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (2)   
   KEGG REACTION (2)   
Gene (2)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (1)   
Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
Protein domain (11)   
   InterPro (11)   
All databases (21)   

Download RDF
DBGET integrated database retrieval system