Entry
Name
sulfide-dependent adenosine diphosphate thiazole synthase;
Thi4 (ambiguous)
Class
Transferases;
Glycosyltransferases;
Pentosyltransferases
BRITE hierarchy
Sysname
NAD+:glycine ADP-D-ribosyltransferase (sulfide-adding)
Reaction(IUBMB)
NAD+ + glycine + sulfide = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O [RN:
R12026 ]
Reaction(KEGG)
Substrate
Product
nicotinamide [CPD:
C00153 ];
ADP-5-ethyl-4-methylthiazole-2-carboxylate [CPD:
C20784 ];
H2O [CPD:
C00001 ]
Comment
This iron dependent enzyme, found in most archaea, is involved in the biosynthesis of thiamine phosphate. The homologous enzyme from plants and fungi (EC
2.4.2.60 , cysteine-dependent adenosine diphosphate thiazole synthase) uses an intrinsic cysteine as sulfur donor and, unlike the archaeal enzyme, is a single turn-over enzyme.
History
EC 2.4.2.59 created 2018
Pathway
Orthology
K22699 sulfide-dependent adenosine diphosphate thiazole synthase
Genes
MPHL : MPHLCCUG_05062(ksdD_3)
VAI : BU251_06310 BU251_06360
MIG : Metig_0735 Metig_1112
MEL : Metbo_0536 Metbo_1265
MEW : MSWAN_0974 MSWAN_1853
METH : MBMB1_1137 MBMB1_1486
MFI : DSM1535_0665 DSM1535_2031
MCUB : MCBB_1020 MCBB_1725
METO : CIT02_02510 CIT02_10600
MEME : HYG87_01485 HYG87_07505
PYA : PYCH_06240 PYCH_08650
THV : ADU37_CDS17350 ADU37_CDS22280
MMET : MCMEM_0306 MCMEM_0840
MELO : J7W08_02430 J7W08_11625
MEZ : Mtc_1604(thi4-1) Mtc_2398(thi4-2)
PDL : Pyrde_0220 Pyrde_0347
» show all
Taxonomy
Reference
Authors
Zhang X, Eser BE, Chanani PK, Begley TP, Ealick SE
Title
Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases.
Journal
Sequence
Reference
Authors
Eser BE, Zhang X, Chanani PK, Begley TP, Ealick SE
Title
From Suicide Enzyme to Catalyst: The Iron-Dependent Sulfide Transfer in Methanococcus jannaschii Thiamin Thiazole Biosynthesis.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.2.59
ExPASy - ENZYME nomenclature database: 2.4.2.59