Entry
Name
N,N'-diacetyllegionaminate synthase;
neuB (gene name);
legI (gene name)
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
phosphoenolpyruvate:2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose 1-(2-carboxy-2-oxoethyl)transferase
Reaction(IUBMB)
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O = N,N'-diacetyllegionaminate + phosphate [RN:
R10304 ]
Reaction(KEGG)
Substrate
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose [CPD:
C20424 ];
phosphoenolpyruvate [CPD:
C00074 ];
H2O [CPD:
C00001 ]
Product
N,N'-diacetyllegionaminate [CPD:
C20418 ];
phosphate [CPD:
C00009 ]
Comment
Requires a divalent metal such as Mn2+. Isolated from the bacteria Legionella pneumophila and Campylobacter jejuni, where it is involved in the biosynthesis of legionaminic acid, a virulence-associated, cell surface sialic acid-like derivative.
History
EC 2.5.1.101 created 2012
Pathway
ec00520 Amino sugar and nucleotide sugar metabolism
Orthology
K18430 N,N'-diacetyllegionaminate synthase
Genes
LWA : SAMEA4504053_1066(spsE)
LANT : TUM19329_12800(neuB)
TSE : THMIRHAS_08930(spsE)
EPS : L0Y14_02440 L0Y14_02510
THIN : CRN91_03145(neuB) CRN91_03215
DEE : HQN60_14860 HQN60_14875
SSEI : FJR45_00975(neuB) FJR45_04420(neuB)
CCOC : CCON33237_1630(legI)
CHV : CHELV3228_1655(legI)
CAMZ : CVIC12175_0322(legI)
PEF : A7E78_05520 A7E78_05530
MEKU : HUW50_13940(neuB) HUW50_13960
EFU : HMPREF0351_10916(neuB)
CPRF : K7H06_04875 K7H06_14850(neuB)
CBAR : PATL70BA_2643(legI)
HOR : Hore_16950 Hore_17000
HALS : D7D81_05095(neuB) D7D81_17935(neuB)
IFN : GM661_06780(neuB) GM661_18095 GM661_18120(neuB)
RML : FF011L_46220(neuB_1)
LAJ : A0128_07320 A0128_07375
LMAY : DPV73_15970(neuB) DPV73_16020
COPR : Cop2CBH44_22070(legI)
CHIH : GWR21_01080(neuB) GWR21_20995(neuB)
SDJ : NCTC13534_05285(spsE_1) NCTC13534_05286(spsE_2)
AGD : FRZ59_07155(neuB) FRZ59_07170
MHAA : Q3Y49_12135 Q3Y49_14225(neuB)
FOK : KK2020170_01100(neuB)
KOS : KORDIASMS9_03609(legI)
KAN : IMCC3317_05800(legI)
CJG : NCTC13459_02715(spsE)
CANT : NCTC13489_01541(spsE)
NLI : G3M70_03950 G3M70_11130(neuB)
MEW : MSWAN_1357 MSWAN_1360
NDV : NDEV_0150(NeuB) NDEV_0210(neuB2) NDEV_0212
» show all
Taxonomy
Reference
Authors
Glaze PA, Watson DC, Young NM, Tanner ME
Title
Biosynthesis of CMP-N,N'-diacetyllegionaminic acid from UDP-N,N'-diacetylbacillosamine in Legionella pneumophila.
Journal
Sequence
Reference
Authors
Schoenhofen IC, Vinogradov E, Whitfield DM, Brisson JR, Logan SM
Title
The CMP-legionaminic acid pathway in Campylobacter: biosynthesis involving novel GDP-linked precursors.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 2.5.1.101