KEGG ENZYME: 2.5.1.128

ENZYME: 2.5.1.128

Entry

EC 2.5.1.128                Enzyme

Name

N4-bis(aminopropyl)spermidine synthase

Class

Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

Sysname

S-adenosyl 3-(methylsulfanyl)propylamine:spermidine 3-aminopropyltransferase [N4-bis(aminopropyl)spermidine synthesizing]

Reaction(IUBMB)

2 S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = 2 S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine (overall reaction) [RN:R11154];
(1a) S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = S-methyl-5'-thioadenosine + N4-aminopropylspermidine [RN:R11159];
(1b) S-adenosyl 3-(methylsulfanyl)propylamine + N4-aminopropylspermidine = S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine [RN:R11160]

Reaction(KEGG)

R11154 R11159 R11160

Substrate

S-adenosyl 3-(methylsulfanyl)propylamine [CPD:C01137];
spermidine [CPD:C00315];
N4-aminopropylspermidine [CPD:C21009]

Product

S-methyl-5'-thioadenosine [CPD:C00170];
N4-bis(aminopropyl)spermidine [CPD:C21010];
N4-aminopropylspermidine [CPD:C21009]

Comment

The enzyme, characterized from the thermophilic archaeon Thermococcus kodakarensis, synthesizes the branched-chain polyamine N4-bis(aminopropyl)spermidine, which is required for cell growth at high-temperature. When spermine is used as substrate, the enzyme forms N4-aminopropylspermine.

History

EC 2.5.1.128 created 2014

Orthology

K07057

N4-bis(aminopropyl)spermidine synthase

Genes

TID:	Thein_2228

TOP:	TOPB45_1193

TCM:	HL41_00370

THET:

F1847_06500

CTHI:

THC_1142

TAV:	G4V39_04970

TMAI:

FVE67_06940

GTK:	GT3570_09820

GGH:	GHH_c21380

GEA:	GARCT_00701

GSR:	GS3922_12390

GZA:	IC807_06460

PTB:	DER53_08120

CTHU:

HUR95_08205

HYI:	K2M58_02480

PABS:

JIR001_06140 JIR001_09810

DKU:	Desku_2563

TTE:

TTE1898

TEX:	Teth514_1121

THX:

Thet_1792

TPD:	Teth39_0634

TIT:

Thit_1686

TMT:	Tmath_1670

TBO:	Thebr_0649

TWI:	Thewi_1855

TKI:	TKV_c17620

CHY:

CHY_0365

ADG:

Adeg_0209

TOC:

Toce_1644

CSC:

Csac_1231

ATE:

Athe_2198

COB:	COB47_1979

CHD:	Calhy_0562

COW:	Calow_1887

CKI:	Calkr_0377

CLC:	Calla_1981

CKN:	Calkro_0440

CCHA:

ELD05_03145

CDIA:

CaldiYA01_03930

CNAG:

OTJ99_000578

CMOR:

OTK00_001998

CDAN:

SOJ16_002319

FCZ:	IMF26_08255

TALU:

JDY09_01080

THUG:

KNN16_00465(speD)

TTH:

TT_C0171

TTJ:	TTHA0539(TTHA0539)

TTS:	Ththe16_0538

TTL:	TtJL18_1537

TPAR:

AV541_07995

TLI:

Tlie_1345

AMO:	Anamo_0349

RMR:

Rmar_0533

RMG:	Rhom172_0537

AAE:

aq_1754

HYA:	HY04AAS1_0834

HHO:	HydHO_0829

HYS:	HydSN_0847

HTH:

HTH_1277

HTE:	Hydth_1269

TAL:

Thal_1053

TRD:	THERU_06865

SUL:	SYO3AOP1_0154

SAF:	SULAZ_0710

PMX:	PERMA_1439

TAM:	Theam_0292

DTE:	Dester_0229

TTK:

TST_0020

TTA:	Theth_1513

CPO:	COPRO5265_0690

CEX:

CSE_05650

BANA:

BARAN1_0480(bpsA)

BIH:	BIP78_1087

FSH:	Q2T83_15425

MJA:	MJ_0675 MJ_1273

MFE:	Mefer_0046 Mefer_0624

MVU:	Metvu_0675 Metvu_0723

MFS:	MFS40622_0658 MFS40622_1505

MIF:	Metin_0014 Metin_1435

MJH:	JH146_0452 JH146_1223

MESG:

MLAUSG7_0027(bpsA) MLAUSG7_0362

MESA:

MLASG1_1207(bpsA) MLASG1_1541

MIG:	Metig_0730 Metig_0858

MMP:

MMP1657

MMQ:	MmarC5_1752

MMX:	MmarC6_1016

MMZ:	MmarC7_0929

MMD:

GYY_09140

MMAK:

MMKA1_18890

MMAO:

MMOS7_17700

MMAD:

MMJJ_11750(bpsA)

MAE:

Maeo_0142

MVN:	Mevan_0957

MVO:

Mvol_1306

MOK:	Metok_0901

METF:

CFE53_00405 CFE53_00505

AFU:

AF_1611

AFG:	AFULGI_00018620

APO:	Arcpr_0370

AVE:	Arcve_0369

AST:	Asulf_00865

FPL:

Ferp_1880

GAC:

GACE_1398

GAH:

GAH_01086

PFU:

PF1111

PFI:

PFC_04775

PHO:	PH0728(PH0728)

PAB:

PAB0872

PYN:

PNA2_1371

PYA:	PYCH_02980

PYS:

Py04_0736

PYC:	TQ32_03155

TKO:

TK1691

TON:

TON_1358

TGA:

TGAM_0371

TSI:

TSIB_0126

TBA:	TERMP_01367

THE:

GQS_08760

THA:

TAM4_1077

THM:

CL1_1351

TLT:

OCC_06976

THS:

TES1_1386

TNU:

BD01_1992

TEU:

TEU_04560

TGY:	X802_05695

THV:	ADU37_CDS00470

TCH:	CHITON_1122

TPEP:

A0127_06790

TPIE:

A7C91_02470

TGG:	A3K92_06945

TCE:	A3L02_04365

TBS:	A3L01_07525

THH:	CDI07_08060

TSL:	A3L11_00505

TTD:	A3L14_09890

TPRF:

A3L09_00360

TRL:	A3L10_06925

TPAF:

A3L08_00720

THY:	A3L12_01265

TIC:	FH039_10455

TCQ:	TIRI35C_1826(bpsA)

THEM:

FPV09_10415

THEI:

K1720_03715

PPAC:

PAP_09495

ABI:

Aboo_0499

ACF:	AciM339_0545

LOB:	NEF87_000160

PSYT:

DSAG12_02754

» show all

Reference

1 [PMID:24610711]

Authors

Okada K, Hidese R, Fukuda W, Niitsu M, Takao K, Horai Y, Umezawa N, Higuchi T, Oshima T, Yoshikawa Y, Imanaka T, Fujiwara S

Title

Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles.

Journal

J Bacteriol 196:1866-76 (2014)
DOI:10.1128/JB.01515-14

Sequence

[tko:TK1691]

Other DBs

ExplorEnz - The Enzyme Database:

2.5.1.128

IUBMB Enzyme Nomenclature:

2.5.1.128

ExPASy - ENZYME nomenclature database:

2.5.1.128

BRENDA, the Enzyme Database:

2.5.1.128

DBGET integrated database retrieval system