KEGG   ENZYME: 2.5.1.136
Entry
EC 2.5.1.136                Enzyme                                 
Name
2-acylphloroglucinol 4-prenyltransferase;
PT-1 (gene name);
PT1L (gene name);
aromatic prenyltransferase (ambiguous);
dimethylallyl-diphosphate:2-acylphloroglucinol 4-dimethylallyltransferase
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
prenyl-diphosphate:2-acylphloroglucinol 4-prenyltransferase
Reaction(IUBMB)
prenyl diphosphate + a 2-acylphloroglucinol = diphosphate + a 2-acyl-4-prenylphloroglucinol [RN:R11609]
Reaction(KEGG)
R11609
Substrate
prenyl diphosphate [CPD:C00235];
2-acylphloroglucinol [CPD:C21498]
Product
diphosphate [CPD:C00013];
2-acyl-4-prenylphloroglucinol [CPD:C21499]
Comment
The enzyme, characterized from hop (Humulus lupulus), acts on phlorisovalerophenone, phlormethylbutanophenone, and phlorisobutanophenone during the synthesis of bitter acids. It also acts with much lower activity on naringenin chalcone. Forms a complex with EC 2.5.1.137, 2-acyl-4-prenylphloroglucinol 6-prenyltransferase, which catalyses additional prenylation reactions. Requires Mg2+.
History
EC 2.5.1.136 created 2017
Orthology
K21590  2-acylphloroglucinol 4-prenyltransferase
Reference
1  [PMID:22166201]
  Authors
Tsurumaru Y, Sasaki K, Miyawaki T, Uto Y, Momma T, Umemoto N, Momose M, Yazaki K
  Title
HlPT-1, a membrane-bound prenyltransferase responsible for the biosynthesis of bitter acids in hops.
  Journal
Biochem Biophys Res Commun 417:393-8 (2012)
DOI:10.1016/j.bbrc.2011.11.125
  Sequence
[ag:BAJ61049]
Reference
2  [PMID:25564559]
  Authors
Li H, Ban Z, Qin H, Ma L, King AJ, Wang G
  Title
A heteromeric membrane-bound prenyltransferase complex from hop catalyzes three sequential aromatic prenylations in the bitter acid pathway.
  Journal
Plant Physiol 167:650-9 (2015)
DOI:10.1104/pp.114.253682
  Sequence
[ag:AJD80254]
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.136
IUBMB Enzyme Nomenclature: 2.5.1.136
ExPASy - ENZYME nomenclature database: 2.5.1.136
BRENDA, the Enzyme Database: 2.5.1.136

  All links  
Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (3)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (2)   
Protein sequence (5)   
   UniProt (2)   
   SWISS-PROT (2)   
   RefSeq(pep) (1)   
DNA sequence (1)   
   RefSeq(nuc) (1)   
Protein domain (3)   
   InterPro (3)   
All databases (18)   

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