KEGG   ENZYME: 2.5.1.149
Entry
EC 2.5.1.149                Enzyme                                 
Name
lycopene elongase/hydratase (flavuxanthin-forming);
crtEb (gene name);
dimethylallyl-diphosphate:all-trans-lycopene dimethylallyltransferase (hydrating, flavuxanthin-forming)
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
prenyl-diphosphate:all-trans-lycopene prenyltransferase (hydrating, flavuxanthin-forming)
Reaction(IUBMB)
(1) prenyl diphosphate + all-trans-lycopene + acceptor + H2O = nonaflavuxanthin + reduced electron acceptor + diphosphate [RN:R12283];
(2) prenyl diphosphate + nonaflavuxanthin + acceptor + H2O = flavuxanthin + reduced electron acceptor + diphosphate [RN:R12284]
Reaction(KEGG)
R12283 R12284
Substrate
prenyl diphosphate [CPD:C00235];
all-trans-lycopene [CPD:C05432];
acceptor [CPD:C00028];
H2O [CPD:C00001];
nonaflavuxanthin [CPD:C22056]
Product
nonaflavuxanthin [CPD:C22056];
reduced electron acceptor;
diphosphate [CPD:C00013];
flavuxanthin [CPD:C22057]
Comment
The enzyme, characterized from the bacterium Corynebacterium glutamicum, is bifunctional. It catalyses the elongation of the C40 carotenoid all-trans-lycopene by attaching an isoprene unit at C-2, as well as the hydroxylation of the new isoprene unit. The enzyme acts at both ends of the substrate, forming the C50 carotenoid flavuxanthin via the C45 intermediate nonaflavuxanthin. cf. EC 2.5.1.150, lycopene elongase/hydratase (dihydrobisanhydrobacterioruberin-forming).
History
EC 2.5.1.149 created 2018
Orthology
K23155  lycopene elongase/hydratase (flavuxanthin-forming)
Genes
SCIAHUG15_19465
CGLCgl0620(Cgl0620)
CGBcg0717(crtEb)
CGUWA5_0594(CrtEb)
CGTcgR_0740
CGSC624_03875(ubiA)
CGGC629_03875(ubiA)
CGMcgp_0717(crtEb)
CGJAR0_03700(ubiA)
CGQCGLAR1_03560(ubiA)
CGXSB89_03315(ubiA)
CEFCE0637(crtEb)
CHNA605_03535(ubiA)
CCNH924_00345(ubiA)
CMDB841_05595(ubiA)
CFNCFAL_10280(ubiA)
CVTB843_10365(ubiA)
CUVCUREI_00105(ubiA)
CHMB842_00595(ubiA)
CMQB840_00375(ubiA)
CMVCMUST_03130(ubiA)
CTEDCTEST_03075
CDXCDES_03195(ubiA)
CSTACSTAT_10575(ubiA)
CCJZccrud_03140(ubiA)
CFCCFLV_10475(ubiA)
CPEGCPELA_00975
CEECENDO_09950
CSANE3227_00065
CGKCGERO_00975
CRLNCTC7448_00519(ubiA)
CCHOCCHOA_00110
CPSOCPPEL_00910
CSURN24_0758(ubiA)
CCYSSAMEA4530656_2401(ubiA)
CYZC3B44_06280
CUOCUROG_08910
CQNG7Y29_03570
CCOECETAM_00640
COKCOCCU_07355
CWKIA203_00750
CFGCFREI_03330
CFACCFAEC_02680
CATRCATRI_02910 CATRI_10290
CHANCHAN_00205
CGFCGUA_00070
CAPPCAPP_09505
CSUEQP029_06860
BFVC628_03535(ubiA)
SNKCP967_33620
NAEIGCM126_12280
ACAONF551_13220
AZHMUK71_13160
AGEGMUG94_14205
AKON9A08_12980
AZXN2K95_13100
AJRN2K98_13805
PDELJCQ34_14720
PNDPla175_07110
UAMUABAM_00068
FYAKMW28_27395
AICJK629_03455
 » show all
Reference
1  [PMID:11432736]
  Authors
Krubasik P, Kobayashi M, Sandmann G
  Title
Expression and functional analysis of a gene cluster involved in the synthesis of decaprenoxanthin reveals the mechanisms for C50 carotenoid formation.
  Journal
Eur J Biochem 268:3702-8 (2001)
DOI:10.1046/j.1432-1327.2001.02275.x
  Sequence
[cgb:cg0717]
Reference
2  [PMID:22963379]
  Authors
Heider SA, Peters-Wendisch P, Wendisch VF
  Title
Carotenoid biosynthesis and overproduction in Corynebacterium glutamicum.
  Journal
BMC Microbiol 12:198 (2012)
DOI:10.1186/1471-2180-12-198
  Sequence
[cgb:cg0717]
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.149
IUBMB Enzyme Nomenclature: 2.5.1.149
ExPASy - ENZYME nomenclature database: 2.5.1.149
BRENDA, the Enzyme Database: 2.5.1.149

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