KEGG ENZYME: 2.5.1.73

ENZYME: 2.5.1.73

Entry

EC 2.5.1.73                 Enzyme

Name

O-phospho-L-seryl-tRNA:Cys-tRNA synthase;
SepCysS;
Sep-tRNA:Cys-tRNA synthase

Class

Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

Sysname

O-phospho-L-seryl-tRNACys:hydrogen sulfide 2-aminopropanoate transferase

Reaction(IUBMB)

O-phospho-L-seryl-tRNACys + sulfide = L-cysteinyl-tRNACys + phosphate [RN:R08577]

Reaction(KEGG)

R08577

Substrate

O-phospho-L-seryl-tRNACys;
sulfide [CPD:C00283]

Product

L-cysteinyl-tRNA(Cys) [CPD:C03125];
phosphate [CPD:C00009]

Comment

In organisms like Archaeoglobus fulgidus lacking EC 6.1.1.16 (cysteine---tRNA ligase) for the direct Cys-tRNACys formation, Cys-tRNACys is produced by an indirect pathway, in which EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) ligates O-phosphoserine to tRNACys, and EC 2.5.1.73 converts the produced O-phospho-L-seryl-tRNACys to Cys-tRNACys. The SepRS/SepCysS pathway is the sole route for cysteine biosynthesis in the organism [1]. Methanosarcina mazei can use both pathways, the direct route using EC 6.1.1.16 (cysteine---tRNA ligase) and the indirect pathway with EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) and EC 2.5.1.73 [2].

History

EC 2.5.1.73 created 2009

Pathway

ec00970

Aminoacyl-tRNA biosynthesis

Orthology

K06868

Sep-tRNA:Cys-tRNA synthetase

Genes

MJA:

MJ_1678

MFE:	Mefer_0975

MVU:	Metvu_1276

MFS:	MFS40622_0574

MIF:	Metin_0086

MJH:	JH146_1495

MESG:

MLAUSG7_1199

MESA:

MLASG1_0578

MIG:	Metig_1345

MMP:

MMP1240

MMQ:	MmarC5_0351

MMX:	MmarC6_1433

MMZ:	MmarC7_0486

MMD:

GYY_07035

MMAK:

MMKA1_14150(pscS) MMKA1_14320(pscS) MMKA1_14490(pscS) MMKA1_14660(pscS)

MMAO:

MMOS7_13480(pscS)

MMAD:

MMJJ_16050(pscS)

MAE:

Maeo_0681

MVN:	Mevan_0554

MVO:

Mvol_0651

MOK:	Metok_0264

METF:

CFE53_03625(pscS)

MTH:

MTH_1067

MMG:	MTBMA_c14550(pscS)

MWO:	MWSIV6_1453

MTEE:

MTTB_03670

METC:

MTCT_0968

METE:

tca_01031(iscS)

METZ:

METMT2_1082

METK:

FVF72_06255(pscS)

MTHM:

FZP57_06960(pscS)

METJ:

FZP68_03675(pscS)

MARO:

MarbSA_16340

MEL:	Metbo_2382

MEW:	MSWAN_0264

METH:

MBMB1_0325

MFC:	BRM9_1944(pscS)

MFI:	DSM1535_0132

MCUB:

MCBB_0296

MSUB:

BK009_11800

METN:

BK008_07435

METT:

CIT01_03215(pscS)

METO:

CIT02_05225(pscS)

MEME:

HYG87_04490(pscS)

MFEG:

GCM10025860_12110

MFV:

Mfer_0642

MKA:

MK0229

AFU:	AF_0028 AF_0181

AFG:	AFULGI_00000260

APO:	Arcpr_0056

AVE:	Arcve_1020

AST:	Asulf_02152

FPL:

Ferp_2023

GAC:

GACE_1091

GAH:	GAH_00355 GAH_02061

MBA:	Mbar_A1638

MBY:	MSBRM_2913

MBW:	MSBRW_3093

MBAR:

MSBR2_2871

MBAK:

MSBR3_0854 MSBR3_1924

MAC:

MA_0722

MMA:

MM_1881

MMAZ:

MmTuc01_1956

MMJ:	MSMAS_2806

MMAC:

MSMAC_1632

MVC:	MSVAZ_1419 MSVAZ_2569

MEK:	MSKOL_1368 MSKOL_2576

MLS:	MSLAZ_0389

METM:

MSMTP_2705

MEF:	MSWH1_2638

MEQ:	MSWHS_2397

MSJ:	MSSAC_0667

MSZ:	MSSIH_0610

MSW:	MSSIT_0641

MTHR:

MSTHT_0797 MSTHT_1396

MTHE:

MSTHC_1892 MSTHC_2472

MHOR:

MSHOH_3505

MFZ:	AOB57_006010(pscS)

MBU:	Mbur_0796 Mbur_1440

MMET:

MCMEM_0673 MCMEM_0928 MCMEM_1925

MELO:

J7W08_01470(pscS) J7W08_03050(pscS) J7W08_08590(pscS)

MMH:	Mmah_0384 Mmah_0699 Mmah_1745

MHAZ:

BHR79_01585 BHR79_04975

MPOT:

BKM01_01065

MEV:	Metev_1356

MZH:	Mzhil_1846

MPY:	Mpsy_1019 Mpsy_2114

MZI:	HWN40_11620(pscS)

MMAV:

RE476_06830(pscS) RE476_12370(pscS)

MSEB:

RE474_08485(pscS) RE474_12495(pscS)

MHZ:	Metho_0600 Metho_2240

MEHF:

MmiHf6_15840

MEES:

MmiEs2_01280

MTP:

Mthe_0102

MCJ:	MCON_0570(pscS)

MHI:

Mhar_0427

MHU:	Mhun_0071 Mhun_2180

MRTJ:

KHC33_04530(pscS) KHC33_15315(pscS)

MLA:	Mlab_0348 Mlab_1214

MEM:	Memar_1614

MBG:	BN140_0832

MEMA:

MMAB1_1094

MCHK:

MchiMG62_18000

MSUM:

OH143_02280(pscS)

MRC:	R6Y96_07420(pscS)

MPI:

Mpet_1028

MEND:

L6E24_11860(pscS)

MEFW:

F1737_06595(pscS) F1737_11540(pscS)

MAQE:

RJ40_10755(pscS)

MFK:	E2N92_11000(pscS)

MOU:	OU421_01745(pscS)

MESB:

L1S32_05260(pscS)

MANQ:

L1994_02105(pscS) L1994_03705(pscS)

MBN:	Mboo_1050 Mboo_2045

MFO:	Metfor_1987 Metfor_2473

MPL:	Mpal_1163 Mpal_1209

MPD:

MCP_0857

MEZ:	Mtc_1599 Mtc_2256

RCI:	RCIX1553 RCIX2270

MELU:

MTLP_12980

BARC:

AOA65_1061

BARB:

AOA66_0045

» show all

Reference

1 [PMID:17512006]

Authors

Fukunaga R, Yokoyama S

Title

Structural insights into the second step of RNA-dependent cysteine biosynthesis in archaea: crystal structure of Sep-tRNA:Cys-tRNA synthase from Archaeoglobus fulgidus.

Journal

J Mol Biol 370:128-41 (2007)
DOI:10.1016/j.jmb.2007.04.050

Sequence

[afu:AF_0028]

Reference

2 [PMID:18559341]

Authors

Hauenstein SI, Perona JJ

Title

Redundant synthesis of cysteinyl-tRNACys in Methanosarcina mazei.

Journal

J Biol Chem 283:22007-17 (2008)
DOI:10.1074/jbc.M801839200