KEGG ENZYME: 2.5.1.96

ENZYME: 2.5.1.96

Entry

EC 2.5.1.96                 Enzyme

Name

4,4'-diapophytoene synthase;
dehydrosqualene synthase;
DAP synthase;
C30 carotene synthase;
CrtM

Class

Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

Sysname

farnesyl-diphosphate:farnesyl-diphosphate farnesyltransferase (15-cis-4,4'-diapophytoene-forming)

Reaction(IUBMB)

2 (2E,6E)-farnesyl diphosphate = 15-cis-4,4'-diapophytoene + 2 diphosphate (overall reaction) [RN:R07652];
(1a) 2 (2E,6E)-farnesyl diphosphate = diphosphate + presqualene diphosphate [RN:R00702];
(1b) presqualene diphosphate = 15-cis-4,4'-diapophytoene + diphosphate [RN:R09793]

Reaction(KEGG)

R00702 R07652 R09793

Substrate

(2E,6E)-farnesyl diphosphate [CPD:C00448];
presqualene diphosphate [CPD:C03428]

Product

15-cis-4,4'-diapophytoene [CPD:C16144];
diphosphate [CPD:C00013];
presqualene diphosphate [CPD:C03428]

Comment

Requires Mn2+. Typical of Staphylococcus aureus and some other bacteria such as Heliobacillus sp.

History

EC 2.5.1.96 created 2011

Pathway

ec00906

Carotenoid biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K10208

4,4'-diapophytoene synthase

Genes

SOF:	NCTC11214_04446(crtM)

FAU:	Fraau_3211

DJA:	HY57_12655

DYE:	EO087_12875

DCS:	ISN74_17645

DTL:	H8F01_19310

DTA:	DYST_00504

PNB:	NK667_27420

AVN:	Avin_20770

AVL:	AvCA_20770

AVD:	AvCA6_20770

TTU:	TERTU_3241

MCA:	MCA0813(sqs)

METU:

GNH96_04645

MMEO:

OOT43_06740

MMT:	Metme_3473

MDN:	JT25_015470

MDH:	AYM39_06145

MKO:	MKLM6_1251

METL:

U737_05055

MPAD:

KEF85_15845

MELL:

IVG45_22125

MRP:	NM686_004040

MMOT:

QZJ86_06485

MAH:	MEALZ_3096

MBUR:

EQU24_15365

MPSY:

CEK71_04650

MMAI:

sS8_2361

MSZE:

MSZNOR_4075

MMOB:

F6R98_20830

MEIN:

methR_P0254

MOZ:	MoryE10_13360

MISZ:

MishRS11D_16140

MESL:

KKZ03_12955

MEIY:

MIN45_P1533

MCAU:

MIT9_P1120

TIG:

THII_3156

BLEP:

AL038_08740

NOC:

Noc_1321

NHL:

Nhal_1310

NWA:

Nwat_1203

NWR:	E3U44_16805

NTT:

TAO_1297

NTG:	NSCAC_0562

AEH:

Mlg_2690

HHA:

Hhal_2280

HHK:	HH1059_01750

AFY:	BW247_04725

NAX:	HC341_00715

HCO:	LOKO_03350(crtB)

HALO:

BWR19_10810

TAU:

Tola_2974

ACII:

C4901_05410

ATY:	A9R16_011080

PNU:

Pnuc_0189

PDQ:	CL55_00001930

POH:	DPM16_01020

PTRP:

DCO17_00980

PANI:

DCO16_00970

PPAL:

AOC06_00990

PCOS:

C2747_01000

PIU:	AOC20_01015

PWU:	A8O14_01025

PARD:

DN92_00960

GLC:	JQN73_08475

BBAG:

E1O_05320

NEU:	NE1958(fdfT)

NET:

Neut_0403

NIT:	NAL212_2368

NII:	Nit79A3_1556

NCO:	AAW31_03940

NUR:	ATY38_00425

NST:	Nstercoris_01057

BPRC:

D521_0190

MCG:

GL4_3107

SDO:	SD1155_02590

AFR:

AFE_2125

AFE:	Lferr_1784

ACU:

Atc_2241

ACZ:	Acaty_c2081

AFI:	Acife_1269

AFJ:	AFERRID_02590

ATX:	GCD22_00867(erg9)

AFP:	K1Y48_00785

ACIY:

MQE22_03700

MFB:	MFUL124B02_14115

CCX:	COCOR_01776(crtB1)

SUR:	STAUR_5419

AGE:	AA314_00534

AVM:	JQX13_13900

MBD:	MEBOL_003418

CFUS:

CYFUS_000054

BPU:

BPUM_1012

BPUM:

BW16_05805

BPUS:

UP12_05415

BIF:	N288_02865 N288_07345

BWH:	A9C19_15130

BXI:	BK049_02840

BALT:

CFN77_05410

BAER:

BAE_12970

BSAF:

BSL056_05360

BACW:

QR42_05250

BACQ:

DOE78_06250

BZH:	NF868_04815

BHAI:

KJK41_21500

BCO:	Bcell_3989

BHK:	B4U37_06815

NTX:	NQZ71_26155

BMQ:

BMQ_0656

BMD:

BMD_0659

BMEG:

BG04_2948

BLE:	BleG1_1581

SHUA:

PQ477_11055

BPF:	BpOF4_14560(crtM)

BKO:	CKF48_16150

CGOT:

J1899_01865

CSPG:

LS684_05850

OIH:

OB2460

AFL:

Aflv_2199

AGN:	AFK25_03145

HHD:	HBHAL_2762(crtM)

HMN:	HM131_12855

HLI:

HLI_02860

HSAN:

MUN89_15050

HAMY:

MUO15_10550

VHL:	BME96_14535

VIK:	KFZ58_17675

FPN:	ABE65_002085

SJE:	AAV35_007825

BMUR:

ABE28_002615

PPSR:

I6J18_04210

PSYH:

D0S48_20350

GSM:	MUN87_19310

SALE:

EPH95_02290

POF:	GS400_07990

PCHU:

QNI29_08895

MLIT:

KDJ21_000495

RMF:	D5E69_08435

RAZ:	U9J35_07990

BBEV:

BBEV_1362(crtB)

BSE:

Bsel_2213

MCUI:

G8O30_03230

PSUA:

FLK61_30285

ECTO:

MUG87_16910

AHAL:

FTX54_007650

SAU:	SA2349(crtM)

SAV:	SAV2562(crtM)

SAW:	SAHV_2546(crtM)

SAH:	SaurJH1_2638

SAJ:	SaurJH9_2584

SAM:	MW2483(crtM)

SAS:

SAS2448

SAC:	SACOL2577(crtM)

SAX:	USA300HOU_2557(crtM)

SAA:	SAUSA300_2499(crtM)

SAO:	SAOUHSC_02879

SAE:	NWMN_2462(crtM)

SAD:	SAAV_2628(crtM)

SUU:	M013TW_2536

SUV:	SAVC_11690

SUE:	SAOV_2607c

SUJ:	SAA6159_02458

SUK:	SAA6008_02601

SUC:	ECTR2_2415

SUT:	SAT0131_02773

SUQ:	HMPREF0772_10627(crtM)

SUZ:	MS7_2569(crtM)

SUD:	ST398NM01_2613

SUX:	SAEMRSA15_24650(crtM)

SUW:	SATW20_26840(crtM)

SUG:

SAPIG2613

SUF:	SARLGA251_23370(crtM)

SAUA:

SAAG_00383

SAUE:

RSAU_002403(crtM)

SAUN:

SAKOR_02554

SAUS:

SA40_2316(crtM)

SAUU:

SA957_2400(crtM)

SAUG:

SA268_2478(crtM)

SAUZ:

SAZ172_2662(crtM)

SAUT:

SAI1T1_2019070

SAUJ:

SAI2T2_1019080

SAUK:

SAI3T3_1019070

SAUQ:

SAI4T8_1019080

SAUV:

SAI7S6_1019070

SAUW:

SAI5S5_1019010

SAUX:

SAI6T6_1019020

SAUY:

SAI8T7_1019050

SAUF:

X998_2545

SAB:	SAB2435c(crtM)

SUY:	SA2981_2499(crtM)

SAUB:

C248_2620(crtM)

SAUM:

BN843_25990

SAUC:

CA347_2638(crtM)

SAUR:

SABB_03587(crtM)

SAUI:

AZ30_13425

SAUD:

CH52_06190

SAMS:

NI36_12820

SHA:	SH0490(crtM)

SHH:	ShL2_00391

SCA:

SCA_2274

SLG:	SLGD_00187

SLN:	SLUG_01850(crtM)

SWA:	A284_01655

SPAS:

STP1_1064

SXY:	BE24_11830 BE24_13820

SXL:	SXYLSMQ121_0053

SXO:	SXYL_00053(crtM)

SCAP:

AYP1020_1733(crtM)

SEQO:

SE1039_25790

SSIF:

AL483_11050

SCV:	A4G25_08790

SPET:

CEP67_08110

SNL:	BJD96_00595

SHOM:

EGX58_06060

SCAR:

DWB96_01945

SCHR:

DWB92_00430

SARL:

SAP2_00930(crtM)

SPIC:

SAMEA4384060_0212(crtM)

SSH:	NCTC13712_02518(crtM)

SSIM:

SAMEA4384339_2368(crtM)

SDB:	CNQ82_01945

SLLO:

ISP08_00320

SSAC:

I6I31_02605

SPSD:

JMB28_00830

STAS:

HYI43_02290

SURY:

MUA21_00265

SDEV:

Q2T90_05250

SROT:

ML435_12320

SSCU:

CEP64_13170

MLEN:

H3V22_08595

MACR:

BHM04_10280

MBRU:

MGG13_00645

MEPI:

KFV12_00565

MEQU:

KFV11_00690

MBOC:

QSV55_01040

ESI:

Exig_0737

EAT:	EAT1b_2492

EAN:

Eab7_0710

EXM:	U719_03905

EXU:	ESP131_06325

EAP:	KB235_12910

EACE:

KKI46_04100

EAUR:

NMQ00_11645

EMM:	PTI97_03575

EPF:	OE059_11125

PMS:	KNP414_02935

PMQ:	PM3016_3175

PMW:

B2K_16060

PBD:	PBOR_33895

PAEN:

P40081_35225

PAEQ:

R50912_31855

PAEA:

R70723_30720

PAEE:

R70331_29475

PBV:	AR543_22770

PXL:	BS614_00350

PSWU:

SY83_14340

PBAC:

HUB98_14530

PTRI:

KDC22_30850

PAUN:

MJA45_20700

COHN:

KCTCHS21_30310(pys)

SACA:

FFV09_08620

PLN:	Plano_2716

PKU:	AUO94_02185

PRT:	AUC31_12725

PLL:	I858_009370

PANA:

BBH88_05395

PDG:	BCM40_01755

PHC:	BBI08_10750

PPLA:

BBI15_09590

PFAE:

AJGP001_15435

PLX:	CW734_15690

PMAT:

BBI11_15120

PDEC:

H1Q58_14405

PGQ:	FK545_11585

JEO:

JMA_13810

SPOR:

SporoP33_08885

SPOP:

SporoP37_09275

SURE:

SporoP32a_09255

SPOS:

DV702_13355

PLAY:

DNR44_006395

SPAB:

KQ224_03165

LPL:	lp_3263(crtM)

LPJ:	JDM1_2617(crtM)

LPT:	zj316_3092(crtM)

LPS:	LPST_C2687(crtM)

LPR:	LBP_cg2662

LPZ:

Lp16_2569

LPB:	SH83_13615

LARG:

LPA65_13980

LLE:	AYR59_01030

FMT:	M3M35_04445

FCP:	M3M37_06690

EHR:

EHR_05365

ECAS:

ECBG_00592

EMU:

EMQU_2797

EDU:

LIU_01925

EGA:	AL523_15525

EGV:	EGCR1_04840

EIE:	ENLAB_05020

TEY:	GLW17_04545

TOO:	C7K38_02270

TKR:	C7K43_09400

AUI:	APT62_02920

AUH:	AWM75_08050

CRN:	CAR_c19230(crtM)

CAW:	Q783_08815

CVD:	LHA31_09530

MARR:

BKP56_12410

JEP:	BW721_07655

JDA:	BW727_102020(crtM)

JPO:	G7058_05440

JEM:	LZ578_02805

DINC:

QNK01_06850

ELM:

ELI_1118

EMT:	CPZ25_008620

ELIM:

B2M23_13180

AWI:	CHL1_000150

LGG:	QJR73_09610

ACL:

ACL_1415

AOC:	Aocu_02610

AHK:	NCTC10172_00272(crtM)

ABRA:

BN85313750(crtB)

APAL:

BN85410530(crtB)

AAXA:

NCTC10138_01637(crtM)

ELE:

Elen_2267

EYY:	EGYY_04210(ERG9)

EGD:	GS424_012835

RTS:	CE91St31_24600(crtM)

MTAR:

DF168_00500(crtB)

OCS:	OF820_08595

NVA:	G3M78_15135

» show all

Reference

1 [PMID:12426357]

Authors

Umeno D, Tobias AV, Arnold FH

Title

Evolution of the C30 carotenoid synthase CrtM for function in a C40 pathway.

Journal

J Bacteriol 184:6690-9 (2002)
DOI:10.1128/JB.184.23.6690-6699.2002

Sequence

[sao:SAOUHSC_02879]

Reference

2 [PMID:16020541]

Authors

Pelz A, Wieland KP, Putzbach K, Hentschel P, Albert K, Gotz F.

Title

Structure and biosynthesis of staphyloxanthin from Staphylococcus aureus.

Journal

J Biol Chem 280:32493-8 (2005)
DOI:10.1074/jbc.M505070200

Sequence

[sae:NWMN_2462]

Reference

3 [PMID:16269684]

Authors

Ku B, Jeong JC, Mijts BN, Schmidt-Dannert C, Dordick JS

Title

Preparation, characterization, and optimization of an in vitro C30 carotenoid pathway.

Journal

Appl Environ Microbiol 71:6578-83 (2005)
DOI:10.1128/AEM.71.11.6578-6583.2005

Sequence

[sao:SAOUHSC_02879]

Reference

4 [PMID:18276850]

Authors

Liu CI, Liu GY, Song Y, Yin F, Hensler ME, Jeng WY, Nizet V, Wang AH, Oldfield E

Title

A cholesterol biosynthesis inhibitor blocks Staphylococcus aureus virulence.

Journal

Science 319:1391-4 (2008)
DOI:10.1126/science.1153018

Sequence

[sau:SA2349]

Other DBs

ExplorEnz - The Enzyme Database:

2.5.1.96

IUBMB Enzyme Nomenclature:

2.5.1.96

ExPASy - ENZYME nomenclature database:

2.5.1.96

BRENDA, the Enzyme Database:

2.5.1.96

DBGET integrated database retrieval system